Preparation method for monoclonal antibody of riemerella anatipestifer

Abstract

The invention provides a preparation method for a monoclonal antibody of riemerella anatipestifer. The method comprises the following specific steps: (1) expanding the culture of the riemerella anatipestifer, inactivating formaldehyde, and then preparing suspension by using PBS to complete antigen preparation; (2) mixing the newly prepared antigen with a freund's complete adjuvant uniformly and stirring for 4 hours until a mixture is emulsified, then performing subcutaneous injection on mice, mixing the newly prepared antigen with the freund's incomplete adjuvant uniformly and stirring for 4 huntil a mixture is emulsified after two weeks of immunization, performing subcutaneous injection on the mice again to obtain immunized mice after one week of immunization, and detecting antibody titer is 1 to 6400; (3) performing cell fusion on the spleen cells of the immunized mice and SP2 / 0 cells; 4) performing positive screening and cloning on fusion cells in step (3). According to the method,the antibody titer is increased by improving the immune effect, so that the immunization times are reduced, thereby achieving the effects of saving time and reducing materials.

Description

technical field [0001] The disclosure relates to the field of biotechnology, in particular to a method for preparing a monoclonal antibody against R. anatipestifer. Background technique [0002] R. anatipestifer belongs to the genus R. anatipestifer, formerly known as Pasteurella anatipestifer. It is an acute, contact and septic infectious disease of domestic ducks, geese, turkeys and other poultry and wild birds. It is also called new duck. disease, duck septicemia, duck plague syndrome, duck plague septicemia and duck infectious serositis. The incidence rate of R. anatipestifer infection can reach 10%-60%, and the mortality rate is 30%-90%. It is one of the infectious diseases that cause serious economic losses in the duck industry. [0003] There are many ducks in our country, and Richteria anatipestifer disease occurs in almost every farm. It occurs all year round and has no obvious seasonality; the effect of drug treatment is not reliable, and often the drug is stopped...

AI Technical Summary

Problems solved by technology

In the routine immunization procedure in the step of immunizing animals, it is usually necessary to immunize once every 2 weeks, and the titer of the antibody can reach the standard after 4 times of immunization, and then proceed to the preparation of hybridomas in 3 days, so this time-consuming process defect

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