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Vaccine reagent kit for preventing carious tooth and method of use thereof

A kit and dental caries technology, applied in the field of vaccine kits for the prevention of dental caries, can solve the problems of serious side effects, can not be applied to human beings, and achieve strong immunogenicity, better immune effect, and strong specificity

Inactive Publication Date: 2009-04-22
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Freund's incomplete adjuvant and complete adjuvant, which are the most widely used in animal experiments, are a class of powerful TH1 cell activators that can induce effective cellular and humoral immunity, but cannot be used in humans due to their severe side effects

Method used

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  • Vaccine reagent kit for preventing carious tooth and method of use thereof
  • Vaccine reagent kit for preventing carious tooth and method of use thereof
  • Vaccine reagent kit for preventing carious tooth and method of use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A vaccine kit for preventing dental caries, comprising an antigen and an adjuvant, the antigen being a recombinant plasmid co-expressing the surface protein antigen PAc of Streptococcus mutans caries and the glucosyltransferase GTF of Streptococcus mutans caries, the adjuvant being Salmonella flagellin.

[0034] The vaccine kit for preventing dental caries provided by the present invention, because the active component of the vaccine adopted is the recombinant plasmid encoding the surface protein antigen PAc and glucosyltransferase GTF of Streptococcus mutans, can encode the surface protein antigen PAc of Streptococcus mutans The exogenous genes of GTF and glucosyltransferase are directly introduced into the host cells, and the immune system of the host is induced to react to the surface protein antigen PAc and GTF of Streptococcus mutans to prevent dental caries. The vaccine has strong immunogenicity, can stimulate a comprehensive and durable immune response, and has s...

Embodiment 2

[0039] A vaccine kit for preventing dental caries, comprising an antigen and an adjuvant, the antigen being a recombinant plasmid co-expressing the surface protein antigen PAc of Streptococcus mutans caries and the glucosyltransferase GTF of Streptococcus mutans caries, the adjuvant being Salmonella flagellin; the plasmid vector of the recombinant plasmid is pVAX.

[0040] In other embodiments of the vaccine kit for preventing dental caries of the present invention, the plasmid vector of the recombinant plasmid can also be pcDNA3.0 or pcDNA3.1. pVAX, pcDNA3.0 or pcDNA3.1 meet the standards of the US FDA, and their use will not pose a safety threat to the users. The plasmid carrier of the recombinant plasmid described in the vaccine kit for preventing dental caries is most preferably pVAX, and the preventive effect of pVAX, pcDNA3.0 or pcDNA3.1 is the best.

Embodiment 3

[0042] The preparation method of the antigen in the dental caries prevention kit comprises:

[0043] (a) Treat the targeted fusion anti-caries eukaryotic expression plasmid pGJA-P with restriction enzymes NheI and NotI, excise the targeted fusion gene (including CTLA-4-Ig target gene, GLU, A-P fragment), and the target The fusion gene was cloned into the pVAX1 vector, digested to confirm the correctness of the construction, and named as pGJA-P / VAX.

[0044] (b) introducing the vector with the gene of interest into Escherichia coli DH5a competent cells;

[0045] (c) Cultivate the target strain on a large scale, and use the EndoFree Plasmid Maxi Kit to extract the plasmid in the target strain (the large plasmid QIAGEN kit has the function of removing endotoxin);

[0046] (e) using a spectrophotometer to measure the concentration of the purified plasmid DNA;

[0047] (f) Use Limulus reagent to detect whether the endotoxin content of the purified plasmid DNA exceeds the standard...

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Abstract

The invention relates to a vaccine reagent kit for preventing decayed tooth, which comprises antigen and adjuvant, wherein the antigen is a dna recombinant plasmid of at least one surface proteantigen and at least one glucosyltransferase which co-express decayed tooth streptococcus mutans; and the adjuvant is flagellins of one or more than one germ. The vaccine reagent kit for preventing the decayed tooth has strong immunogenicity, can excite comprehensive and lasting immune response, and has strong specificity, even immune effect and simple and convenient operation. The invention also provides an application method for the vaccine reagent kit for preventing the decayed tooth, and the vaccine reagent kit has good immune effect and simple and convenient operation.

Description

technical field [0001] The invention relates to the technical field of oral medicine prevention, in particular to a vaccine kit for preventing dental caries and a method for using the vaccine kit. Background technique [0002] At present, caries is still a global problem that threatens human oral health. It is the goal pursued by many caries researchers to find a simple, economical and effective method of preventing caries. The research on immune caries prevention has a history of 40 years, has accumulated a large number of theoretical and practical achievements, and is still developing. [0003] In the 1960s, a large number of epidemiological and etiological studies showed that Streptococcus mutans is the main cariogenic microorganism in humans, and its colonization in the oral cavity is closely related to the occurrence of caries. In 1965, Thomas and colleagues reported that the IgA system is the primary specific immune component in saliva. After decades of extensive res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/09A61K39/39A61P1/02
Inventor 鄢慧民刘芳杨菁毅石伟
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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