294 results about "Streptococcus mutans" patented technology

The present invention provides compositions, medicaments, and methods for the treatment or prophylaxis of conditions associated with the binding of Streptococcus mutans to teeth. Specifically, the present invention provides a method for preventing dental caries and infective endocarditis in a subject by treating the subject's oral cavity with a composition or medicament comprising the competence stimulating peptide (CSP) of the causative bacterium, which inhibits the ability of the said bacteria to attach to the surface of teeth. Novel anti-caries compositions using the CSP are also disclosed.

Strains of Lactobacillus that have been selected for their capability of reducing the number of Streptococcus mutans in the mouth of mammals through inhibiting activity in combination with good binding to the oral mucins and dental plaque, Administering the strains in a product such as food, reduces and treats dental caries. Preferred strains are Lactobacillus reuteri strain ATTC PTA-4965 or Lactobacillus reuteri strain ATTC PTA-4964.

The invention provides an oral antibacterial composition containing nisin and polylysine. According to the composition, the growth of oral pathogenic bacteria such as porphyromonas gingivalis, actinobacillus actinomycetemcomitans, streptococcus mutans, lactic acid bacillus, staphylococcus aureus and candida albicans can be effectively inhibited, and tooth decay, gingivitis, periodontitis, ozostomia and the like can be prevented; the composition can be prepared into toothpaste, mouth wash, refreshing and tasting chips and chewing gum which are used for preventing and treating oral diseases. Meanwhile, the invention provides a preparation method of the compositions such as the toothpaste, the mouth wash, the refreshing and tasting chips and the chewing gum. The oral antibacterial composition prepared through the method can prevent and treat various oral diseases and has no toxic and side effect and allergy or stimulation phenomenon on a human body.

The invention discloses lactobacillus rhamnosus. A strain (lactobacillus rhamnosus) LR863 is preserved in China General Microbiological Culture Collection Center (CGMCC) and the preservation number isCGMCC No. 14410. The lactobacillus rhamnosus disclosed by the invention has remarkable bacterium-inhibition and sterilization effects on oral pathogenic microorganisms including streptococcus mutans,streptococcus gordonii, porphyromonas gingivalis, fusobacterium nucleatum, actinobacillus actinomycetemcomitans and the like, and has the effects of reducing formation of dental plaque and reducing occurrence and development of oral diseases including tooth decay, periodontitis and the like. A lactobacillus rhamnosus preparation disclosed by the invention can comprise viable bacterium thalli of the lactobacillus rhamnosus, dead bacterium thalli of the lactobacillus rhamnosus, metabolic products of the lactobacillus rhamnosus and intracellular extract, is an oral probiotics preparation which has wide application and a remarkable effect, and can be widely used as an additive of liquid-state foods, solid-state foods, colloidal foods, oral hygiene products and oral treatment drugs.

The invention discloses lactobacillus plantarum KLDS1001 and application of the lactobacillus plantarum KLDS1001 to inhibition of streptococcus mutans, belonging to the technical field of microorganisms. The lactobacillus plantarum KLDS1001 provided by the invention is collected in China general microbiological culture collection center (CGMCC) with the address of institute of microbiology of Chinese academy of sciences, 1# yard, beichen road west, chaoyang district, Beijing; the collection number is CGMCC No.9066; the collection date is April 16th, 2014. The lactobacillus plantarum KLDS1001 has an inhibition effect on the streptococcus mutans, can serve as an additive to be added to health drugs and such oral health products as chewing gum and is used for oral health; the lactobacillus plantarum KLDS1001 can serve as one of ingredients of oral cleaning products to be added into toothpaste and mouth wash for cleaning the oral cavity and reducing the number of streptococcus mutans in the oral cavity; a probiotic therapy method is developed for preventing and treating oral diseases such as decayed teeth.

The invention discloses ab analysis method of rejection capability of lactobacillus plantarum metabolite and application of the lactobacillus plantarum metabolite. The effective extract of lactobacillus plantarum FB-T9 contains micromolecular bacteriocin substances and hydrogen peroxide ingredients, so that bacteriostatic activity for streptococcus mutans in floating state is provided and relatively bacteriostatic activity can be provided under biological membrane state to destroy the integrity of the biological membrane structure. The lactobacillus plantarum FB-T9 is applied to fermented food or pharmaceutical compositions containing metabolite of the lactobacillus plantarum FB-T9, is used for preventing, decreasing or treating tooth decay and has wide application prospect.

New strains of Lactobacillus that have been selected for their capability of improved reduction the number of Streptococcus mutans in the mouth of mammals through inhibiting activity in combination with better binding to the oral mucins and dental plaque, thereby preventing, reducing or treating dental caries, and products derived from said strains, including agents for treatment or prophylaxis of caries for administration to humans.

The invention relates to a vaccine reagent kit for preventing decayed tooth, which comprises antigen and adjuvant, wherein the antigen is a dna recombinant plasmid of at least one surface proteantigen and at least one glucosyltransferase which co-express decayed tooth streptococcus mutans; and the adjuvant is flagellins of one or more than one germ. The vaccine reagent kit for preventing the decayed tooth has strong immunogenicity, can excite comprehensive and lasting immune response, and has strong specificity, even immune effect and simple and convenient operation. The invention also provides an application method for the vaccine reagent kit for preventing the decayed tooth, and the vaccine reagent kit has good immune effect and simple and convenient operation.

The present invention relates to a microorganism belonging to the group of lactic acid bacteria characterized in that it is capable of specifically binding to Streptococcus mutans, wherein the specific binding is (i) resistant to heat treatment; and / or (ii) resistant to protease treatment; and / or (iii) calcium-dependent; and / or (iv) formed within a pH range between 4.5 and 8.5; and / or (v) formed in the presence of saliva. Preferably, the specific binding can be assayed as follows: (a) growing said microorganism to stationary phase; (b) mixing said microorganism with Streptococcus mutans which has been grown to stationary phase; (c) incubating the mixture obtained in step (b) under conditions allowing the formation of aggregates of said microorganism and Streptococcus mutans and (d) detecting aggregates by the occurrence of a pellet. Another aspect of the present invention is an analog or fragment of said microorganism which is thermally inactivated or lyophilized, wherein said analog or fragment retains the capability of specifically binding to Streptococcus mutans. In addition, the present invention encompasses compositions and additives for food, feed or drinks comprising the microorganism belonging to the group of lactic acid bacteria which specifically bind to Streptococcus mutans or an analog or fragment thereof. Moreover, uses of said microorganism or said analog or fragment thereof for the preparation of an anticariogenic or pharmaceutical composition or anticariogenic food or feedstuff as well as methods for producing said compositions or food or feedstuff are provided by the present invention.

The invention discloses an IgY antibody preparation having mixed specificity for resistance to streptococcus mutans and streptococcus sobrinus. The IgY antibody preparation is prepared by mixing 10-70 parts by weight of streptococcus mutans bacteria and bacteria pieces and 30-90 parts by weight of streptococcus sobrinus bacteria and bacteria pieces into an antigen via laying hens. The invention also discloses a preparation method of the IgY antibody preparation having the mixed specificity for resistance to the streptococcus mutans and the streptococcus sobrinus, and a toothpaste which contains the IgY antibody preparation having the mixed specificity for resistance to the streptococcus mutans and the streptococcus sobrinus and the IgY antibody preparation having the mixed specificity for resistance to the anti-porphyromonas gingivalis and the fusobacterium nucleatum and is used for preventing saprodontia, gingivitis and halitosis thereof. The toothpaste used for preventing saprodontia, gingivitis, periodontitis and halitosis halitosis thereof has obvious effects for preventing saprodontia, gingivitis, periodontitis and halitosis bacteria thereof.

The invention discloses a strain of caries resistant lactobacillus plantarum K41 and application thereof. Lactobacillus plantarum K41 provided by the invention is preserved in Common Microorganism Center of Chinese Microorganism Strain Preservation Management Committee, with preservation number of CGMCC No.15462, and preservation date of March 20, 2018. The strain is separated from farmyard self-made pickles in Yanan Road, Shunqing District, Nanchong, Sichuan, grows well on an MRS agar culture medium, can inhibit growth of streptococcus mutans UA159, forming of exopolysaccharide, degradation of hydroxyapatite, and forming of a biological film, has good auto-agglutination and copolymerization capability, is good in tolerance for antibiotics, salt and acid, and is easy to be planted in the oral cavity. Animal experiment shows that K41 can inhibit rat enamel demineralization and caries degree, can be applied to functional food, and has very important meaning for body health.

The present invention discloses a nutritional composition for improving oral health. The nutritional composition comprises the following raw materials in parts by weight: 5-30 parts of probiotics, 20-80 parts of prebiotics and 0.1-2 parts of epigallocatechin gallate; the probiotics are selected from at least one of lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus helveticus, and lactobacillus salivarius; and the prebiotics are selected from at least one of inulin, oligofructose, galactooligosaccharide, xylooligosaccharide, stachyose, oligoisomaltose, raffinose, trehalose, and lactitol. At the same time, the present invention also provides a use of the nutritional composition in preparations of oral health-care products. The provided nutritional composition can inhibit colonization of caries inducing streptococcus mutans, reduces adhesion of subgingival gingiva pathogenic bacteria, inhibits bad breath, promotes the proliferation of the oral probiotics, prevents the occurrence and development of dental erosion and oral cancers, and can effectively solve the oral problems caused by alcoholism, smoking, staying up late, irregular diet, long-term consumption of acidic substances, etc.

The application provides a lactobacillus paracasei PC-01 isolated from sour yak milk in Longren Township, Dangxiong County, Lhasa, Tibet, wherein the microbial preservation number of the lactobacillus paracasei PC-01 is CGMCC No.17537. The Lactobacillus paracasei PC-01 can improve oral health flora imbalance, has probiotics with better acid tolerance and bile salt tolerance, has probiotic characteristics, can be directly taken as a preparation for improving oral microecological environment, or as a raw material to prepare a microbial antagonist for pathological states in oral cavity, wherein the pathological state microorganisms comprise at least one of streptococcus mutans, Porphyromonas gingivalis, Treponema denticola, Genus Prevotella and Fusobacterium nucleatum.

The invention discloses a novel thiazole compound capable of inhibiting streptococcus mutans. The compound number is XQH-3-6, the molecular formula is C16H16ClN5O4S, the molecular weight is 409.85, and the Chinese name is N1-(3-chlorphenyl-N4-(5-nitrothiazole-2-yl))piperidine-1,4-dicarboxamide. Experiments show that the compound presents the good antibacterial activity and fungicidal activity on type strains and clinical strains of the streptococcus mutans in a floating state. Meanwhile, when the final concentration of the compound XQH-3-6 in a culture medium reaches 4 mg / L, the inhibition ratio of streptococcus mutans biological membrane reaches 97% or above. The compound is small in molecular weight and simple in structure, inhibition experiments prove that the compound has the advantages of being high in inhibition, good in killing effect and the like, growth of main pathogen-streptococcus mutans buoyant cells of caries and formation of a biological membrane can be significantly inhibited, the potential of preventing the caries is achieved, and the compound can serve as a novel targeted candidate drug for preventing the caries.

The invention provides lactobacillus plantarum SD26 as well as a product and application thereof, and relates to the field of microorganisms. The lactobacillus plantarum SD26 provided by the inventionhas a preservation number of CGMCC No.19328, is derived from pickled vegetables, has an obvious inhibiting effect on oral pathogenic bacteria including streptococcus mutans, candida albicans, fusobacterium nucleatum and porphyromonas gingivalis and food-borne pathogenic bacteria including salmonella typhimurium, escherichia coli, staphylococcus aureus and listeria monocytogenes, and has good application potential in the aspects of prevention and treatment of the oral pathogenic bacteria and the food-borne pathogenic bacteria.

Water-forming NADH oxidase derived from Streptococcus mutans should be further improved in terms of stability for practical use in industrial production. An object of the present invention is to provide an enzyme that is obtained through modification of a water-forming NADH oxidase, which is useful as an NAD+ regeneration system for stereoselective oxidation catalyzed by an oxidoreductase, by protein engineering techniques so that the enzyme can withstand long-term use without exhibiting a reduction of its activity for the regeneration of NAD+, that is, an enzyme having improved stability, and to provide a method for efficiently producing a useful substance such as an optically active alcohol or amino acid. The present invention relates to an enzyme modification method that can improve the stability of water-forming NADH oxidase derived from Streptococcus mutans by appropriately introducing mutation.

The present invention provides compositions, medicaments, and methods for the treatment or prophylaxis of conditions associated with the binding of Streptococcus mutans to teeth. Specifically, the present invention provides a method for preventing dental caries and infective endocarditis in a subject by treating the subject's oral cavity with a composition or medicament comprising the competence stimulating peptide (CSP) of the causative bacterium, which inhibits the ability of the said bacteria to attach to the surface of teeth. Novel anti-caries compositions using the CSP are also disclosed.

The invention discloses a probiotics toothpaste composition capable of improving the health of oral cavity. The composition comprises a first toothpaste component and a second toothpaste component; wherein the first toothpaste component comprises probiotics and mannan oligosaccharide, and the second toothpaste component comprises enzymes, mannatide, and a diester type surfactant; and the first toothpaste component and the second toothpaste component are individually packed, and the first toothpaste component and the second toothpaste component are mixed and blended when being used on the teeth. Mannan oligosaccharide and degradation product of mannatide can provide carbon sources for the growth of probiotics, the pathogenic bacteria in oral cavity cannot utilize mannan oligosaccharide, moreover, mannan oligosaccharide can absorb pathogenic bacteria so as to starve the pathogenic bacteria; at the same time, alkaline polypeptide in degradation product of mannatide can cut off glycan chain of cell walls of bacteria; and the diester type surfactant has a strong cleaning and stain-removing performance, and is capable of effectively remove the residual bacterium bodies. After application, the number of pathogenic bacteria especially streptococcus mutans is greatly reduced, and the health of oral cavity is prominently improved.

The invention discloses an immunoglobulin (IgY) antibody oral spray for preventing decayed teeth. The spray comprises the following components in percentage by weight: 0.1 to 0.5 percent of IgY, 5 to 15 percent of bacteriostat, 3 to 6 percent of preservative, 5 to 20 percent of glycerin, 5 to 10 percent of ethanol, 0.1 to 1 percent of edible essence and 50 to 75 percent of distilled water. The IgY antibody oral spray for preventing the decayed teeth is convenient to manufacture and use, can effectively prevent the decayed teeth and treat gingivitis, periodontitis and canker sore, and has high safety, the bacteriostatic ratio to streptococcus mutans c is over 80 percent, and the bacteriostatic ratio to streptococcus mutans d is over 85 percent. In addition, the IgY antibody oral spray for preventing the decayed teeth can be stably preserved at normal temperature for at least three years.

The invention discloses an antibacterial peptide and application thereof. The antibacterial peptide is an antibacterial peptide YHX-1 with an amino acid sequence as shown in SEQ ID NO. 1. The antibacterial peptide has broad-spectrum antibacterial activity, can inhibit gram-positive bacteria such as listeria monocytogenes, staphylococcus aureus and streptococcus mutans and gram-negative bacteria such as escherichia coli and salmonella, and has high antibacterial activity. In addition, the antibacterial peptide is relatively low in hemolytic activity, relatively low in toxicity, short in synthetic sequence, small in molecular weight and low in chemical synthesis difficulty, the safety is ensured while pathogenic bacteria in a living body can be well and specifically killed, and in addition, the large-scale production cost can also be saved.

The invention relates to the technical field of gene engineering, in particular to novel broad-spectrum chimeric lysin BGS-PlySb and an encoding gene and application thereof. The novel broad-spectrum chimeric lysin BGS-PlySb has an amino acid sequence shown as in SEQ ID NO. 1; the encoding gene of the novel broad-spectrum chimeric lysin BGS-PlySb has a nucleotide sequence shown as in SEQ ID NO. 2. A novel chimeric lysin is constructed by means of gene splicing and is suitable for killing various species of Streptococcus and Staphylococcus, particularly various species of Enterococcus, and Staphylococcus aureus, as well as Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus suis, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus mutans, Streptococcus iniae and other species; the novel broad-spectrum chimeric lysin BGS-PlySb has good stability and is insensitive to high-concentration NaCl, recombinant protein GBS-PlySb is well expressible in Escherichia coli BL21 (DE3), and a high dose of GBS-PlySb is free of significant cytotoxicity. Therefore, the novel broad-spectrum chimeric lysin BGS-PlySb is applicable independently to or as an additive to the control of various species of Streptococcus and the treatment of infections caused by these species, and has a promising application prospect.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus paracasei with the effects of reducing blood sugar, protecting the oral cavity and resisting depression and application of the lactobacillus paracasei. The lactobacillus paracasei nbk-LC16 provided by the invention has relatively strong acid resistance and cholate resistance, is good in tolerance in artificial gastric juice and artificial intestinal juice, and can effectively reduce blood sugar and inhibit streptococcus mutans. The influence of the lactobacillus paracasei nbk-LC16 on hyperglycemia mice is studied, and after the lactobacillus paracasei nbk-LC16 is perfused, compared with model group mice, the weight of the hyperglycemia mice is remarkably increased in 8W, the liver index and the spleen index are remarkably reduced, FBG and PBG are remarkably reduced, the HbA1c and insulin levels are remarkably reduced, and the hepatoglycogen content is increased. Test results show that the lactobacillus paracasei nbk-LC16 can regulate blood glucose by regulating insulin level and HbA1c and promoting conversion of blood glucose into hepatic glycogen. Researches on the inhibitioneffect of the streptococcus mutans strain on streptococcus mutans show that the streptococcus mutans strain can be effectively inhibited through in-vitro tests and oral test results, and the oral cavity and tooth health can be protected; meanwhile, the anti-depression effect is achieved.

The invention belongs to the technical field of biology and specifically discloses a preparation method of a streptococcus mutans inhibitor. The preparation method comprises the following steps: (a) inoculating paenibacillus bovis CGMCC No.8333 into a bran culture medium to be fermented to obtain fermentation liquor; (b) centrifuging the fermentation liquor obtained in the step (a), taking supernatant, putting the supernatant into high-temperature water bath to inactivate protease, adding ammonium sulfate into supernatant after cooling, centrifuging to take precipitate, adding water to dissolve the precipitate and freeze drying to obtain a crude product 1; (c) performing gel column separation on the crude product 1 obtained in the step (b), collecting antibacterial active ingredients and freeze drying to obtain a crude product 2; (d) performing HPLC purification on the crude product 2 obtained in the step (c), collecting antibacterial active ingredients and freeze drying to obtain thestreptococcus mutans inhibitor. By means of the preparation method disclosed by the technical scheme of the invention, varieties and sources of the streptococcus mutans inhibitor are widened; the prepared inhibitor can effectively inhibit streptococcus mutans, staphylococcus aureus and the like and has a better application prospect.

The invention specifically discloses a method for preparing fermented soybean milk powder through lactobacillus fermentum, the prepared fermented soybean milk powder and application. The method comprises the following steps that the lactobacillus fermentum CGMCC No.17321 is inoculated in soybean milk for fermentation, and freezing drying is conducted to obtain the fermented soybean milk powder. The method for preparing the fermented soybean milk powder through the lactobacillus fermentum, the prepared fermented soybean milk powder and the application have the advantages that the obtained fermented soybean milk powder can significantly reduce the formation quantity of biofilms of streptococcus mutans, and it is disclosed that the fermented soybean milk powder of lactobacillus fermentum CGMCC No.17321 / lactobacillus fermentum CGMCC No.17321 and lactobacillus plantarum ATCC 14917 (purchased from ATCC) has new application in dental caries resistance; compared with other strains capable of preparing soybean milk powder and other fermentation culture mediums, the obtained fermented soybean milk powder has a more remarkable effect of inhibiting the formation of the biofilms of the streptococcus mutans, and the stability is good.

A polynucleotide encoding a polypeptide which lowers the glucose side chain amount in a serotype-specific polysaccharide antigen of Streptococcus mutans; novel Streptococcus mutans strain having the above polynucleotide; an antibody specific to this Streptococcus mutans strain; and a method of detecting the above-described Streptococcus mutans strain occurring in a subject sample. According to this method, it is possible to examine the presence or absence of Streptococcus mutans of an untypable serotype in a subject sample to thereby specify a subject having a high risk of the onset of infective endocarditis.

The present application relates generally to a method and a composition matter that provides a rapid and potent antimicrobial photodynamic inactivation (aPDI) of pathogenic bacteria that express high-affinity cell-surface hemin receptors (CSHRs) using Ga(III)-protoporphyrins IX (GaPpIX or Ga-PpIX). The invention provides an effective treatment option for infections of skin or body cavities that are accessible to visible-light irradiation, such as a handheld LED array emitting visible light (405 nm), especially for infections caused by Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA), pathogenic staphylococci, Streptococcus mutans, S. pneumoniae, S. pyogenes, streptococci, corynebacteria, mycobacteria, and Bacillus anthracis.

The invention discloses an antimicrobial peptide. An amino acid sequence thereof is shown as any one of SEQ ID NO:1-SEQ ID NO:7. The antimicrobial peptide disclosed by the invention is capable of moreefficiently restraining activity of streptococcus mutans under floating state and biofilm state; the antimicrobial peptide has a rapid sterilizing effect, low cytotoxicity and high antibiofilm activity; the antimicrobial peptide has the advantages of simple synthesis and low production cost; the antimicrobial peptide is suitable for mass synthesis and is beneficial to clinical conversion.

The invention discloses a sweet-tea tooth protection lozenge, and belongs to the field of tooth protection health care. The sweet-tea tooth protection lozenge can effectively restrain adhesion and acid production of streptococcus mutans so that the effect of well preventing dental caries and the effect of well improving the oral health can be achieved. The sweet-tea tooth protection lozenge comprises food adhesives and filling agents, and further comprises a sweet-tea extract, wherein the sweet-tea extract accounts for 1-30% of the total weight of the sweet-tea tooth protection lozenge. The sweet-tea tooth protection lozenge is used for preventing the dental caries and improving the oral health.

The invention provides an acid-activated CSP targeted antimicrobial peptide, which is prepared by reacting 2,3-dimethylmaleic anhydride (DMMAn), an antimicrobial peptide melittin and a CSP targeted molecule. Moreover, the invention provides a preparation method and two applications of the acid-activated CSP targeted antimicrobial peptide. According to the acid-activated CSP targeted antimicrobial peptide, the selectivity of the antibacterial peptide and the aggregation degree of the antibacterial peptide on the surface of SM (Streptococcus Mutans) bacteria can be improved through the CSP targeted molecule, and the toxicity of the antibacterial peptide melittin is lowered through the protection of the DMMAn on amidogens; and after entering a decayed tooth, the CSP targeted antibacterial peptide can be activated by the acid condition of a microenvironment, so that the CSP targeted antibacterial peptide has sterilizing activity.

The invention discloses mouthwash capable of effectively inhibiting bacteria and a preparation method thereof. The mouthwash is composed of an antibacterial biological extract, sodium bicarbonate, biologically refined glycerin, peppermint oil, lauryl sodium sulfate, a freshener WS-23, an anti-caking agent, a sweetening agent and a mouthwash matrix. Wherein the antibacterial biological extract is natamycin, nisin and phenyllactic acid; natamycin, nisin and phenyllactic acid are weighed, natamycin, nisin and phenyllactic acid are added into purified water, stirring is carried out through a reaction kettle until natamycin, nisin and phenyllactic acid are dissolved, and other components can be added, stirring at a high speed is carried out until all substances are dissolved, and finally packaging is carried out after sterile filling. The mouthwash disclosed by the invention contains natamycin, nisin and phenyllactic acid, the mouthwash can effectively kill and inhibit G<+> and G<->bacteriasuch as porphyromonas gingivalis, actinobacillus actinomycetemcomitan, Fusobacterium, streptococcus mutans and the like in the oral cavity, has a decomposition effect on plaque biofilms generated byflora, can decompose dental plaque while protecting beneficial bacteria, and has the tooth cleaning effect while sterilizing and removing the plaque.