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Isolated Lactobacillus paracasei PC-01 for promoting oral health and application of Lactobacillus paracasei PC-01

A Lactobacillus and paracheese technology, applied in the field of microorganisms, can solve the problems of oral environment damage, oral systemic health damage, etc., and achieve the effects of cleaning oral breath, good proliferation ability, and adjusting the balance of flora

Active Publication Date: 2019-09-20
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned traditional methods seriously damage the oral environment and cannot restore its original balance in a short period of time.
Moreover, if the bacteria in the oral cavity are blindly eliminated, it will break the balance of the normal oral microenvironmental flora, which will damage the oral cavity and the health of the whole body

Method used

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  • Isolated Lactobacillus paracasei PC-01 for promoting oral health and application of Lactobacillus paracasei PC-01
  • Isolated Lactobacillus paracasei PC-01 for promoting oral health and application of Lactobacillus paracasei PC-01
  • Isolated Lactobacillus paracasei PC-01 for promoting oral health and application of Lactobacillus paracasei PC-01

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 thalline growth form and electron micrograph

[0035] (1) Strain activation

[0036] 1. The cultivation of live bacteria of Lactobacillus paracasei PC-01

[0037] After the cryopreservation tube of Lactobacillus paracasei PC-01 preserved at -80°C was enriched and grown at 37°C for 18 hours in MRS liquid medium (sterilized at 121°C for 15 minutes) (logarithmic growth phase), Re-transfer to sterilized MRS medium according to 2% V / V, and finally use the MRS agar plate counting method to count the number of viable bacteria, and collect the microbial cells by centrifugation for later use (logarithmic growth phase).

[0038] 2. Preparation of Lactobacillus paracasei PC-01 inactivated bacteria

[0039] Incubate the live bacteria obtained from the aforementioned 1 culture at 85° C. for 3 minutes to obtain inactivated Lactobacillus paracasei PC-01.

[0040] (2) Bacterial growth morphology and electron microscope photos

[0041] The Lactobacillus paracasei PC-0...

experiment example 2

[0042] Experimental example 2 Antibacterial experiment of Lactobacillus paracasei PC-01

[0043] (1) Activation of pathogenic bacteria

[0044] Streptococcus mutans (S.mutans): aerobic culture at 37°C in 5mL Tryptic soy Broth culture medium, and shake culture at 150rpm for 20 hours;

[0045] Porphyromonas gingivalis (P.gingivalis): cultured anaerobically at 37°C in 5mL Tryptic soy Broth culture medium, and cultured at 150rpm for 4 days;

[0046] Nucleatum (F.nucleatum): cultured anaerobically at 37°C in 5mL Brain-heart infusion medium Broth culture medium, and cultured with shaking at 150rpm for 4 days;

[0047] Actinomycetemcomitans group (A.actinomycetemcomitans) group: cultured anaerobically at 37°C in 5mL Brain-heart infusion medium Broth culture medium, and cultured with shaking at 150rpm for 20 hours.

[0048] (2) Co-cultivation experiment

[0049] Live and inactivated bacteria of Lactobacillus paracasei PC-01 were used as the experimental group, live and inactivate...

experiment example 3

[0091] Experimental example 3 Effect of Lactobacillus paracasei PC-01 on oral micro-ecological environment

[0092] (1) Determination of cohesion ability

[0093] In this example, Lactobacillus paracasei PC-01 was used as the experimental group, and commercial oral probiotic strains Streptococcus salivarius K12 and Lactobacillus reuteri ATCC55730 were used as the control group for comparative experiments.

[0094] 1. Experimental method:

[0095] Evaluated by monitoring the overnight co-culture liquid for a decrease in optical density at 620 nm due to the formation of aggregates and precipitates, an indication of the effect of the selected proprietary probiotic on oral pathogens Reduce positive effects.

[0096] Percent Aggregation Capacity (%AC) values ​​were obtained using Formula II below:

[0097] %AC={1-(OD tf / OD t0 )}100 Formula II

[0098] Among them, OD tf and OD t0 represent the optical density at the final moment and the optical density at the initial mome...

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Abstract

The application provides a lactobacillus paracasei PC-01 isolated from sour yak milk in Longren Township, Dangxiong County, Lhasa, Tibet, wherein the microbial preservation number of the lactobacillus paracasei PC-01 is CGMCC No.17537. The Lactobacillus paracasei PC-01 can improve oral health flora imbalance, has probiotics with better acid tolerance and bile salt tolerance, has probiotic characteristics, can be directly taken as a preparation for improving oral microecological environment, or as a raw material to prepare a microbial antagonist for pathological states in oral cavity, wherein the pathological state microorganisms comprise at least one of streptococcus mutans, Porphyromonas gingivalis, Treponema denticola, Genus Prevotella and Fusobacterium nucleatum.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to an isolated Lactobacillus paracasei PC-01 for promoting oral health and application thereof. Background technique [0002] The oral cavity is a dynamic and balanced microbial environment with multi-flora symbiosis. The types of microorganisms colonized in the oral cavity are complex, including bacteria, fungi, mycoplasma, protozoa, and viruses. Current studies have confirmed that there are more than 700 known species of bacteria in the oral cavity. Only when a good ecological balance is maintained between the microorganisms in the oral cavity, and between the microorganisms and the oral tissues of the host, can the oral cavity maintain a healthy state. If foreign microorganisms or other factors lead to a significant increase in the proportion of individual or several microorganisms inherent in the oral microecology and become the dominant bacteria in the colonization site, espec...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P1/02A61P31/04C12R1/225
CPCA61K35/747A61P1/02A61P31/04C12N1/205C12R2001/225
Inventor 张凌宇马晨马杰杨慧娟郭霄
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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