Egg yolk antibody for anti-chicken-coccidiosis and preparation method and device thereof

Abstract

The invention discloses an egg yolk antibody and a preparation method and device thereof. A test object is used for performing polypide reinvigoration and proliferation on tender eimeriida, giant eimeriida and poisoned eimeriida, after the insects are mixed in equal proportions, antigen is prepared by ultrasonic disruption, the obtained antigen solution is mixed with an equal volume of freund's complete adjuvant and freund's incomplete adjuvant separately, laying hens are immunized three times, egg yolk antibodies in collected egg yolk after three times of immunizationare extracted, purified and identified, and the antibody titer is evaluated; the research on different yolk powder preparation processes is carried out, the effect of lyophilized powder preparation is significantly higher than that of powder spraying technology, the matchingrelationship between the yolk and the dilution, the lyophilization temperature curve and other parameter conditions in the process are prepared, the cost is relatively low, the preparation efficiency is high, antibody loss rate is only 10%, high antibody titer, antibody titer after high-temperature powder spraying shows a high downward trend, and the method is obviously superior to the high-temperature powder spraying technology for preparing yolk powder.

Description

technical field [0001] The present invention relates to the technical field of preparation of egg yolk antibody for preventing chicken coccidiosis, and more particularly to a kind of egg yolk antibody for preventing chicken coccidiosis and a preparation method and device thereof. Background technique [0002] Avian coccidiosis is caused by Eimeria ( Eimeria ) is a parasitic protozoal disease caused by one or more coccidia in the intestinal epithelial cells of chickens. The disease has a global distribution and has caused huge economic losses to the chicken industry. In 1870, Rivolta and Eimer first reported coccidiosis in the intestinal tract of chickens. At present, chicken coccidiosis is one of the most frequent, serious and difficult diseases in intensive chicken farms in my country. For a long time, the main method to control chicken coccidiosis is to use chemical drugs, but coccidiosis is very easy to develop drug resistance, and the phenomenon of coccidiosis outbreak...

AI Technical Summary

Problems solved by technology

However, the existing methods for preparing and purifying egg yolk antibodies still need to be improved. The high-temperature spray method may easily lead to inactivation of egg yolk antibody activity, loss of function, etc.

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