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Rabbit monoclonal antibody preparation method

A monoclonal antibody and antibody technology, which is applied in the preparation methods of peptides, chemical instruments and methods, and anti-animal/human immunoglobulins, etc., can solve the problems of slow preparation, low antibody affinity and low efficiency, etc. Affinity, effects of understanding fundamental issues in immunology

Pending Publication Date: 2019-11-15
福州精锐生物技术有限公司
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Problems solved by technology

[0002] A monoclonal antibody is a highly uniform antibody produced by a single B cell clone that only targets a specific epitope, called a monoclonal antibody. The rabbit antibody library is a very high-quality source of rabbit-derived polyclonal antibodies and monoclonal antibodies. The antibody has the advantages of high specificity, high activity and high affinity. The preparation of rabbit monoclonal antibody in the prior art is through hybridoma technology, which is slow in preparation, not efficient enough, and the antibody affinity is not high. Therefore, a rabbit monoclonal antibody is designed Clonal antibody preparation method is necessary

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  • Rabbit monoclonal antibody preparation method

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Embodiment Construction

[0018] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0019] see figure 1 , the present invention provides a technical solution:

[0020] A method for preparing a rabbit monoclonal antibody, comprising the following steps: Step 1, immune serum preparation; Step 2, antibody affinity purification; Step 3, mass spectrometry; Step 4, antibody variable region gene spectrum acquisition; Step 5, monoclonal Antibody acquisition;

[0021] Among them, in the above step 1, the heteromorphic rabbits with bas mutation and w...

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Abstract

The invention discloses a rabbit monoclonal antibody preparation method. The method comprises the following steps of 1, preparing immune serum; 2, affine purifying an antibody; 3, obtaining a mass spectrum; 4, obtaining an antibody variable region gene spectrum; 5, obtaining the monoclonal antibody, wherein, in step 1, heteromorphic rabbits with bas mutations and wild parents b9 are selected, subcutaneous inoculation of freund's complete adjuvant on the rabbits, immunization is performed every three days, immunization is performed three times, injection is performed one time two weeks after immunization, one month after immunization, NG-XMTTM protein is subjected to intravenous injection at the tails of the rabbits, a sterile small test tube is used for collecting immunized rabbit blood, after marking, the rabbits are delivered to a preparation room, unimmunized rat serum is adopted as control, ELISA is adopted for determining antibody valence, the ELISA is used for determining the OD490 light absorption value quantification and qualitatively judging whether or not the immune serum is positive, and the positive immune serum is selected; the method is more efficient and rapider andhas high affinity.

Description

technical field [0001] The invention relates to the technical field of preparation of rabbit monoclonal antibodies, in particular to a preparation method of rabbit monoclonal antibodies. Background technique [0002] A monoclonal antibody is a highly uniform antibody produced by a single B cell clone that only targets a specific epitope, called a monoclonal antibody. The rabbit antibody library is a very high-quality source of rabbit-derived polyclonal antibodies and monoclonal antibodies. The antibody has the advantages of high specificity, high activity and high affinity. The preparation of rabbit monoclonal antibody in the prior art is through hybridoma technology, which is slow in preparation, not efficient enough, and the antibody affinity is not high. Therefore, a rabbit monoclonal antibody is designed Clonal antibody production methods are necessary. Contents of the invention [0003] The purpose of the present invention is to provide a preparation method of rabbit...

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Application Information

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IPC IPC(8): C07K16/18C07K1/22
CPCC07K16/18C07K2317/92
Inventor 陈黎娜王睿熊凯媛周雪
Owner 福州精锐生物技术有限公司
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