Detection method for antigenic substance in herba houttuyniae injection

A Houttuynia cordata injection and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that the results are not easy to quantify, influence, and can not observe the symptoms of animal allergic reactions, etc., to achieve strong specificity, high sensitivity, The effect of ensuring safety

Inactive Publication Date: 2016-02-03
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

This patented technology describes how certain plants can help reduce harm caused by chemicals that may affect people's health or their environment when they ingest them through food products containing these compounds called histamine. By measuring this response beforehand, researchers have been able to determine if there were any side effects associated with exposure to chemically synthesized drugs like Histidylcholine.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding ways to safely administer certain drugs such as houtuynia Cordta injection without causing adverse side affecting patients or healthcare providers who may also receive them due to their negative impact upon patient outcomes during treatment procedures.

Method used

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  • Detection method for antigenic substance in herba houttuyniae injection
  • Detection method for antigenic substance in herba houttuyniae injection
  • Detection method for antigenic substance in herba houttuyniae injection

Examples

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Embodiment 1

[0013] Example 1 Detection of Houttuynia Antigenic Substances in Houttuynia Injection

[0014] In this study, the possible allergic substances in Houttuynia cordata injection - Houttuynia cordata protein and other antigenic substances were studied, and the ELISA detection method was established. Large size, strong specificity, low price and many other advantages, and can be used to guide the production of enterprises.

[0015] Take Houttuynia cordata, add 7 times the volume of water to reflux and extract 3 times, each time for 3 hours, filter, combine the filtrate, concentrate the filtrate to a clear paste with a relative density of 1.16±0.01 (70°C), add ethanol until the alcohol content is 50wt. %, stand overnight, filter, take the precipitate and dissolve it in water, ultrafilter with an ultrafiltration membrane with a molecular weight cut-off of 10,000, take the retentate, concentrate, and freeze-dry to obtain the houttuynia protein fraction. Bradford method (preparing a s...

Embodiment 2

[0026] Houttuynia cordata injection allergy test detection method comparison of two kinds of processes of embodiment 2

[0027] Original process injection (steam distillation): take Houttuynia cordata, crush it, and pass through No. 2 sieve; weigh 1kg of Houttuynia cordata powder for steam distillation, collect 500mL of initial distillate, and mix 7.5g of 2-hydroxypropyl-β- Dissolve the cyclodextrin in the above initial distillation liquid, stir magnetically at 60°C for 3 hours, add an appropriate amount of sodium chloride to dissolve, dilute the volume to 500 mL with water for injection, filter, and sterilize to obtain the product.

[0028] New process injection (supercritical extraction): take Houttuynia cordata, pulverize it, and pass through a No. 2 sieve; weigh 1kg of Houttuynia cordata powder for supercritical extraction with carbon dioxide, the extraction pressure is 25MPa, the temperature is 45°C, and the extraction is 90min to obtain super Critical extract: add 1.5% 2...

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Abstract

The invention discloses a detection method for an antigenic substance in a herba houttuyniae injection. The method comprises the following steps: performing the steps of extracting, concentrating, ethanol precipitating, performing ultra-filtration and the like on a herba houttuyniae medicinal material or preparation to obtain a herba houttuyniae protein antigenic substance; performing immune treatment on a domestic rabbit through a standard antigen prepared from the herba houttuyniae protein antigenic substance and the Freund's complete adjuvant to obtain a standard serum antibody; detecting the limited quantity of a corresponding residual vegetable protein antigenic substance in a sample to be detected through an enzyme-linked immuno-sorbent assay (ELISA) method. According to the detection method, the ELISA detection method which is high in specificity and high in sensitivity for the herba houttuyniae protein is established for the first time according to the immune reaction mechanism of an antigen and an antibody; the clinical medication safety of the herba houttuyniae injection is fully ensured.

Description

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Claims

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Application Information

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Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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