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Method for preparing bovine origin anti-avian influenza immune globulin

An immunoglobulin and avian influenza technology, which is applied in the field of medicine and achieves the effects of high titer, high promotion and application value, and good curative effect.

Inactive Publication Date: 2008-08-27
TIANJIN SHENGJI GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report about the preparation method of bovine anti-chicken avian influenza serum and immunoglobulin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for preparing bovine-derived anti-chicken avian influenza immunoglobulin is characterized in that the specific implementation steps are as follows:

[0019] (1) Basic immunization, select several healthy cows, and inject 80 portions of chicken avian influenza vaccine intramuscularly into the neck of each cow for the first time to carry out basic immunization of chicken avian influenza virus vaccine. Chicken avian influenza vaccine adopts various immunization methods such as intramuscular injection, intradermal, subcutaneous multi-point injection, and Houhai point injection. The inactivated chicken avian influenza virus cultured by tissue was mixed with Freund's complete adjuvant in a ratio of 1:1 and used as an immunogen to immunize cattle. For the first time, 80 parts of chicken avian influenza vaccine were injected intramuscularly at multiple points in the neck of each cow. Basic immunity.

[0020] (2) 12 days after the basic immunization, carry out booster ...

Embodiment 2

[0025] A method for preparing bovine-derived anti-chicken avian influenza immunoglobulin is characterized in that the specific implementation steps are as follows:

[0026] (1) Preparation of immune plasma: immunize animals with tissue culture inactivated virus, after basic immunization and hyperimmunization, blood collection and separation of plasma.

[0027] (2) Refining and purification: Dilute the prepared immune plasma by 3 times and then concentrate it to a protein content of 3-15% and a titer content of 3000-8000 through conventional preparation processes such as gastric enzyme digestion, heating denaturation, and ultrafiltration. unit / ml. Add hydrochloric acid to the supernatant to adjust to pH 2.0, place at 4.0°C for 2 days to inactivate the virus, then add NaOH to adjust to pH 7.0 to kill other susceptible viruses of different animals.

[0028] (3) Sub-packaging: The present invention can have two types of liquid and freeze-dried, and the specifications are 500IU / bo...

Embodiment 3

[0030] A method for preparing bovine-derived anti-chicken avian influenza immunoglobulin is characterized in that the specific implementation steps are as follows:

[0031] (1) Basic and booster immunization: Glycoside peptide injection 5ml / bottle was used as the immune enhancer to increase the antibody titer; the immune adjuvant was Freund's complete adjuvant mixed with the immunogen at a volume ratio of 1:1.

[0032] Select a few healthy cows, and inject 80 portions of chicken avian influenza vaccine into the neck of each cow for the first time, and carry out the basic immunization of chicken avian influenza virus vaccine. Chicken avian influenza vaccine adopts various immunization methods such as intramuscular injection, intradermal, subcutaneous multi-point injection, and Houhai point injection. The inactivated chicken avian influenza virus cultured by tissue was mixed with Freund's complete adjuvant in a ratio of 1:1 and used as an immunogen to immunize cattle. For the fi...

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PUM

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Abstract

The invention relates to a preparation method of bovine anti chicken avian influenza immunoglobulin, which comprises (1), mixing inactivated chicken avian influenza and freund complete adjuvant cultured via tissue at the mol ratio of 1:1 as immunogen immune bovine, while each cattle is injected with 40-100 portions of chicken avian influenza vaccines at multipoints on neck, (2), after 10-15 days of immunity, processing reinforced immunity via 150-300 portions of chicken avian influenza vaccines, (3), adding antibiotics into blood, standing for 5-10h, separating serum, using natural compresson coagulation method to separate immunoglobulin, depositing impure proteins via ammonium sulfate, cutting Fc segment of pepsin, keeping antibody F(ab) second segment, depositing Fc protein via ammonium sulfate, removing Fc protein, adding hydrochloride to adjust pH to 2.0, standing for 2 days at 4.0DEG C, inactivating virus, adding NaOH to adjust pH 7.0, killing other animal susceptible viruses, (4), filtering and removing bacterial, packing without germ. The invention has simple preparation and simple application.

Description

technical field [0001] The invention belongs to the field of medicine, in particular to a preparation method of bovine-derived anti-chicken avian influenza immunoglobulin. Background technique [0002] At present, chicken viral diseases are still the main infectious diseases that seriously restrict the healthy development of the chicken industry. Viral infectious diseases occur in hundreds of millions of chickens in different seasons every year in the country. Once the chicken is infected with the disease, it has the characteristics of acute onset, severe clinical symptoms, extremely easy to spread, and high mortality and mortality, which is extremely harmful to the breeding industry. Avian influenza is a systemic or respiratory infectious disease of poultry caused by avian influenza virus. Domestic poultry such as chickens, turkeys, ducks, and quails, as well as waterfowl, wild birds, and seabirds, can be infected. Avian influenza is a disease that seriously harms the po...

Claims

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Application Information

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IPC IPC(8): C07K16/10C07K1/14
Inventor 古长庆
Owner TIANJIN SHENGJI GRP CO LTD
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