Immunogen for obtaining Nrf1D protein antibody, Nrf1D protein antibody and Elisa detection kit

A detection kit and immunogen technology, applied in the field of antibodies, to achieve the effect of high specificity and sensitivity

Active Publication Date: 2017-05-24
CHONGQING UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

But the biggest problem at present is that there is no suitable reagent to detect the expression of Nrf1D protein

Method used

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  • Immunogen for obtaining Nrf1D protein antibody, Nrf1D protein antibody and Elisa detection kit
  • Immunogen for obtaining Nrf1D protein antibody, Nrf1D protein antibody and Elisa detection kit
  • Immunogen for obtaining Nrf1D protein antibody, Nrf1D protein antibody and Elisa detection kit

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Embodiment Construction

[0021] The present invention will be described in further detail below in conjunction with specific embodiments. This implementation case is carried out on the premise of the technology of the present invention, and the detailed implementation and specific operation process are given to illustrate the inventiveness of the present invention, but the protection scope of the present invention is not limited to the following examples.

[0022] 1. Experimental materials

[0023] 1. Main reagents:

[0024] Sulfo-SMCC, KLH, and Sulfolink Resin are products of Pierce Company in the United States, and Freund's complete adjuvant and incomplete Freund's adjuvant are from Sigma Company in the United States.

[0025] NaCl, KCl, NaCl 2 HPO 4 12H 2 O. KH 2 PO 4 , Tris, Glycine, EDTA, TMB, DMSO, glutaraldehyde, etc. are domestic analytical reagents.

[0026] 2. Consumables:

[0027] The dialysis bag is a product of Viskase Company in the United States, with a molecular weight cut-off ...

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Abstract

The invention provides an immunogen for obtaining an Nrf1D protein antibody, the Nrf1D protein antibody and an Elisa detection kit. The immunogen is obtained as follows: polypeptide with the amino acid sequence shown in SEQ ID NO.1 is synthesized firstly, Sulfo-SMCC is used as a coupling agent, the polypeptide and KLH (keyhole limpet hemocyanin) are coupled; the antibody is obtained as follows: immunogen is taken as an antigen, the antigen is emulsified with FCA (freund's complete adjuvant) to immunize a New Zealand white rabbit, blood of the immunized New Zealand white rabbit is centrifuged, and a supernatant after centrifugation is collected; the Elisa detection kit comprises components including an ELISA plate, an enveloping solution, a blocking solution, an ELISA secondary antibody, a TMB color developing solution, a stop solution, a detection antigen and the Nrf1D protein antibody, the detection antigen is the polypeptide with the amino acid sequence shown in SEQ ID NO.1, glutaradehyde is used as a coupling agent, and the polypeptide and BSA bovine serum albumin are coupled.

Description

technical field [0001] The invention belongs to the technical field of antibodies, and in particular relates to an immunogen for obtaining Nrf1D protein antibodies, Nrf1D protein antibodies and an Elisa detection kit. Background technique [0002] CNC-bZIP is a subfamily of bZIP transcription factors characterized by an approximately 40 amino acid homology domain adjacent to the N-terminal basic leucine zipper region. From an evolutionary point of view, proteins with this homeodomain have been found in Caenorhabditis elegans (Skn1), Drosophila melanogaster (cap'n'collar), chicken (ECH) [63], mouse (Bach) and human In the process of development, there is a wide range of participation. In humans, the three known members (NF-E2, TCF11 / LCR-F1 / Nrf1, and Nrf2) and their murine homologs have all been shown to lead to a lineage of β-globin loci The process by which the specific expression mechanism is isolated and characterized. However, only NF-E2 is cell-type specific, neither ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C07K16/18G01N33/68
CPCC07K14/00C07K14/795C07K16/18C07K19/00G01N33/6827G01N2333/46
Inventor 张义国汪红霞
Owner CHONGQING UNIV
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