91results about How to "Short modeling time" patented technology

The invention discloses miR-124 gene knockout murine animal models. The murine animal models refer to mice with miR-124-1, miR-124-2 and miR-124-3 genes knocked out. With the adoption of a Crispr-cas9 gene knockout technology, microRNA (micro ribonucleic acid) miR-124 genes with the highest expression quantity in the brains of the mice are knocked out. The obtained mice show obvious nervous system disease states such as reduction of locomotor activity, deterioration of learning and memorizing abilities, increase of soluble amyloid beta protein and the like. Therefore, the simple, reliable and economical animal models can be provided for research of nervous system disease pathology and screening of drugs for treating nervous system diseases.

The invention provides a method for constructing an equivalent circuit model of a lithium ion battery. The method includes the steps that an initial equivalent circuit model of the lithium ion battery is selected and includes multiple unknown parameters; the lithium ion battery is selected and tested so that multiple sets of test data can be obtained; Ohm internal resistance of the initial equivalent circuit model and equivalent capacitance of open-circuit voltage are obtained according to each set of test data; a simulation model of the initial equivalent circuit model is constructed, each set of test data is input into the simulation model, and initial values of the unknown parameters are set; estimation is performed on the unknown parameters through the simulation model and an optimization toolbox, and the estimation is stopped when an error between output voltage of the simulation model and output voltage in each set of test data meets a preset condition so that final values of the unknown parameters can be obtained. The embodiment of the method has the advantages that the equivalent circuit model is rapid to construct, and model constructing complexity is low.

The invention belongs to the field of animal experiment models, and discloses application of a composite high-fat forage to construct a non-alcoholic fatty liver disease rat model. The high-fat forage is composed of the following raw materials in percent by mass: 77.5% of a rat basic forage, 10% of egg, 10% of coconut oil, 2% of cholesterol, 0.5% of bile salt, and 500mg / kg / d of sodium valproate calculated according to the rat weight. After 8 weeks, rats all have typical non-alcoholic fatty live symptoms, and liver has a lot of fat accumulation along with inflammatory cell infiltration. The model establishing time is short, the success rate is high, and the composite high-fat forage is applicable to pathogenesis research induced by high-fat-diet combined medicines with liver-toxicity side effect, screening of related control measures and efficacy evaluation of treatment medicines.

The invention relates to a data-based urban sewage pumping station system modeling method. The conventional modeling for a drainage pipeline network pumping station model has high complexity. The method comprises the following steps: firstly, establishing a predicting model for a drainage pipeline network pumping station; secondly, preprocessing original data acquired by an SCADA system; thirdly, determining a model set type and identifying a model structure and a model parameter; and finally, performing online dynamic modification on the model parameter. The method of the invention breaks through a constraint that mechanism characteristics are required to be understood completely in the conventional modeling, the modeling time is relatively short and the obtained model structure is relatively simple.

The invention discloses a Microstation v8i-based city rapid-modeling method, which comprises the following steps of: performing an aerial survey on a tested area according to a conventional aerial photogrammetric survey method by adopting an aerial photogrammetric survey system; and 2, performing analysis processing on aerial survey data in a Microstation v8i software environment by using a processor, namely, establishing a three-dimensional aerial photogrammetric survey model, performing land feature classification and marking, correspondingly establishing a land feature type layer-color mapping table, performing vector data acquisition, establishing models of houses, scattered trees and land features of other types respectively, processing all the land features in the tested area respectively, and obtaining three-dimensional geometric models of all the land features in the tested area.

The invention discloses an impact condition-considered simplified model modeling method suitable for early-stage design of automobiles. The method comprises the following steps of: carrying out overall structure decomposition on a modeling target automobile; determining a simplified modeling method corresponding to each decomposed structure on the basis of a modeling method provided by ADAMS software; after the simplified modeling method corresponding to each decomposed structure is determined, setting simplified modeling parameters to complete a simplified model construction process of each structure; integrating the constructed simplified models into a whole automobile model; and carrying out impact simulation test on the integrated whole automobile model and importing an adjustment coefficient K to adjust the impact simulation result. According to the method disclosed by the invention, the optimal design of impact simulation in the early-stage design of the automobiles is considered, so that the simulation results of the constructed models are more correct and reasonable, the method is concise, the efficiency is high, and the simulation time is short; and the method is suitable for the impact resistance optimal design in the early stage of structural design of the automobiles.

The invention belongs to the technical field of medical animal model feed and discloses feed for preparation of an alcoholic liver animal model. The feed for mice is prepared from casein, L-cystine, DL-methionine, maltodextrin, meal cellulose, xanthan gum, choline bitartrate, a mineral premix, a vitamin premix, cholesterol, sodium cholate, fish oil, alcohol and water. The feed for preparation of an alcoholic liver animal model can gradually cause mouse alcoholic fatty liver, hepatitis and liver fibrosis. The model has features similar to human diseases and pathology has a certain development process. A modeling method is simple and easy and has a high success rate, a low death rate, good repeatability and a wide application prospect.

The invention discloses a modeling method of an Sjogren syndrome mouse model. The modeling method comprises the following steps: killing mice, taking out bilateral salivary glands and peeling off capsules and connective tissues; washing with PBS (Poly Butylenes Succinate); adding the PBS according to the amount of adding 0.5ml of the PBS into each salivary gland; shearing the salivary glands into pieces, and uniformly homogenizing and centrifuging in an ice bath; then taking supernatant and quantifying salivary gland antigens by adopting a BCA (Bicinchoninic Acid) protein quantifying method; adjusting the concentration of the salivary gland antigens to be 4mg / ml by utilizing the PBS; adding equal quantity of an FCA (Freund Complete Adjuvant) or an FIA (Freund Incomplete Adjuvant) and diluting the concentration to be 2mg / ml; repeatedly blowing and beating until two liquid phases are dissolved mutually to form an ivory color; randomly grouping C57BL / 6 mice and shaving off furs on the backs of the mice; carrying out intradermal multi-site injection of 2mg / ml mouse salivary gland antigens prepared by the FCA on the back and tails of the mice on the current day and the 7th day, wherein the injection amount is 0.1ml per mouse; injecting equal quantity of the salivary gland antigens prepared by the FIA on the 14th day through the same method; after modeling for about 6 weeks, detecting indexes and screening the successfully modeled mice. The modeling method disclosed by the invention is high in modeling efficiency and short in modeling time.

The invention discloses a method for predicting the dissolved oxygen concentration of sewage based on fuzzy clustering supporting a vector regression algorithm, which is used for predicting the content of dissolved oxygen DO in the sewage. Aiming at the problem that the dissolved oxygen is difficult to measure in real time in a sewage treatment process, firstly, a whole sample is divided into a plurality of sub-samples through fuzzy clustering, then, a support vector regression model is established on each sub-sample, and then integration is carried out, and on-line prediction on the content of the dissolved oxygen DO in the sewage is carried out. The method has higher prediction precision, and is superior to other time series prediction methods in the aspect of comprehensive performance,and provides an effective solution for quickly and accurately predicting water quality.

The invention discloses a noninvasive fabricating method for a pulmonary fibrosis animal model. The method comprises the steps: the method that the bacterium solution dropping is poured inside a lower trachea through an endoscope is adopted, and a nasal zero degree 4 # hard-type endoscope, a xenon lamp cold light source and a Panasonic KS-822 video monitoring system are utilized; a rat is placed in a dryer with 26 cm diameter, and the ether anesthesia is carried out on the rat, the 200 microlitre of the medicine bleomycin is ejected inside the trachea, the medicine is fully flowed into the bronchus along with the airflow when inhaling air, elastic fixings clamped on the four limbs of the rat are removed, the rat is placed into a rat cage to wait for the anabiosis, the anabiosis period ranges from 30 sec to 60 sec, and the rat is placed into a constant temperature animal house for feeding; the activity, the feeding and the spirit conditions of the inoculated rat are observed every day, and the corresponding data is recorded.

Disclosed is a method for constructing tree shrew nonalcoholic simple fatty liver animal models. Tree shrew serve as a model animal, a high-fat diet consists of 1000g of normal diets, 20g of cholesterol and 200g of lard and is used for freely feeding the tree shrews, and the tree shrews can drink purified water freely. After seven weeks, 60% of the tree shrews have an obvious fatty liver symptom; after 14 weeks, 80% of the tree shrews have the obvious fatty liver symptom, wherein 20% of the tree shrews have a severe fatty liver symptom, and liver cells of about 95% of the tree shrews have pimelosis; and an analysis result shows that the method is short in modeling time, high in success ratio, safe, reliable and capable of being used for drug research and efficacy assessment of nonalcoholic fatty livers and particularly for the drug research and the efficacy assessment of simple fatty livers.

The invention belongs to the technical field of antenna measurement, and provides an equivalent radiation modeling method based on spherical near-field measurement and a spherical mode source, which is used for overcoming the problems of invariant spatial distribution of ideal dipole, lack of flexibility, low model precision and the like in a conventional modeling method. The near-field measurement data of an antenna sphere to be tested is taken as a basis, the spherical mode source is taken as the basic unit of the equivalent model, spherical wave expansion and pattern matching are utilized,an equivalent model is established to make the model possess the radiation characteristics similar to the antenna to be measured, and the azimuth and elevation angles of the spherical mode source areseparated from each other, and the azimuth angles are optimized only for the spherical mode source. The precision of the model can be improved in a short time by this one-dimensional optimization method, which is of practical value in engineering.

The invention relates to an application of adriamycin and adenine jointly used in preparing animal models used for screening medicine for treating a CKD. According to the invention, the application dose of adriamycin is 2.5-10 mg / Kg each time, and the application dose of adenine is 200 mg / Kg every day. The animal model more accords with the occurrence and development process of the human CKD, experimental results prompt that the model making time can be shortened when the two kinds of medicine are applied jointly, and kidney injury is more serious at the same time point.

The invention provides a constructing method and application for an osteoportic fracture disease animal model. According to the provided technical scheme, based on a constructed osteoporosis animal model, thighbone small lateral incision fracture molding of a model animal is conducted, then a femur fractured end bilateral inverse intramedullary nail inserting internal fixation is conducted, and the osteoportic fracture disease animal model is constructed. The method avoids influences of uncontrollable factors on an experimental result, ensures the uniformity of modeling, shortens the molding time, and accelerates the experiment process. The method is applied to the preparation of the osteoportic fracture disease animal model and is beneficial for meeting related requirements of the osteoportic fracture disease animal model, and the osteoportic fracture disease animal model is taken as a study object to study the pathogenesis, prevention and treatment of basic and clinical osteoportic fractures.

The invention discloses a landslide displacement prediction method based on SVR-LSTM mixed deep learning. The landslide displacement prediction method comprises the steps of 1, performing EMD decomposition on displacement data to obtain an IMF component and a residual term; 2, substituting trend term data into a trend term prediction model SVR for training; and taking residual data as test items and substituting the test items into the trained model to obtain a trend item prediction result; 3, obtaining candidate training attributes of a plurality of periodic terms; 4, calculating mutual information and a Pearson's correlation coefficient and selecting influence factors; 5, using the influence factors as training factors of the LSTM to obtain a plurality of LSTM models; and substituting the residual data into the LSTM model to obtain predicted output values, and adding the predicted output values of the IMF components to obtain a periodic term prediction result; 6, correspondingly adding data in the periodic term prediction result and the trend term prediction result to obtain a total displacement prediction result. The SVR and LSTM methods are adopted to predict the sum, so that the stability and accuracy of the prediction result are greatly improved, the credibility of the prediction result is ensured, and the calculation is efficient.

The invention relates to the field of life medicine, and particularly relates to a construction method of a Kras mutation related oral mucosa malignant animal model. The construction method of the mouse oral mucosa malignant animal model comprises the steps that the model is constructed by adopting inducible Kras genetic engineering mice in combination with 4-nitroquinoline 1-oxide (4NQO) chemicalinduction, and on the premise of achieving the cancer formation target, the modeling time is shortened as much as possible. A traditional 4NQO chemical induction oral squamous carcinoma animal modelhas the defect of being long in consumed time (larger than or equal to 20 weeks). According to the method, oral squamous carcinoma is successfully induced by combining Kras mutation with 4NQO, only 12weeks are needed from induction to cancer formation, and compared with a traditional animal model, the time is saved by 2 months for the whole experiment.

The invention relates to a method for establishing a model of aflatoxin inducing macaque liver fibrosis. The method comprises the following steps: with macaque as a model animal, during the model making, adding 0.5mg / kg aflatoxin to the drinking water of macaque for sucking, twice every month at an interval of 2 weeks for 8 successive months; feeding the macaque with macaque commercial maintenance-period feed containing more than 19% of protein and more than 4% of crude fat; performing pathological analysis every 2 months; and 8 months later, performing pathological detection to show obvious liver fibrosis symptoms. The method provided by the invention is safe and reliable, shortens the model making time of an animal model, and is of great significance to screening and predicting effective drugs for controlling liver fibrosis and even liver cancer as an experimental foundation.

The invention discloses a reliability analysis method of a high-speed railway traction substation system, which comprises the following steps of: 1, defining an evaluation system of the high-speed railway traction substation system, namely, specifying a system range and defining a system reliability index; 2, determining a success guiding criterion and a reliability index of reliability modeling of the high-speed railway traction substation system; 3, taking the electrical main wiring of the high-speed railway traction substation as a research object, determining a GO-FLOW signal flow direction, selecting a proper operator, and establishing a GO-FLOW graph of the electrical main wiring of the traction substation; 4, inputting reliability parameters of each operator; 5, performing quantitative calculation and analysis on each operator of GO-FLOW to obtain steady-state and dynamic reliability indexes of the GO-FLOW; and 6, completing reliability evaluation of the high-speed railway traction substation system.

The invention provides a method for establishing a rhesus monkey hepatic fibrosis model by an intragastric administration method. The method comprises the following steps: selecting two-to-three-year-old male rhesus monkeys with the body weight of 5 to 9 kg as model animals; feeding high-fat feed in a modelling process, enabling the male rhesus monkeys to drink ethyl alcohol aqueous solution with the volume concentration of 9 to 11 percent, and performing intragastric administration on each rhesus monkey to give a CC14 solution in the modelling process, wherein the intragastric administration is performed twice a week and the dosage is 0.5 to 1.5 mL / kg each time, and a model is established successfully after the intragastric administration lasts for 20 to 25 weeks. The method for establishing the rhesus monkey hepatic fibrosis model by the intragastric administration method is simple, safe, reliable, short in modelling time and high in success rate, and can be used for researching an occurrence mechanism of liver diseases.

The invention belongs to the technical field of biology, and particularly relates to a tumor tissue transport fluid and an application thereof. The tumor tissue transport fluid provided by the invention comprises a plurality of antibiotics and nutritional factors, is definite in components, does not contain serum, can significantly improve the defect of nutrient depletion of previous tissue transport fluids, and avoids activity reduction of tumor samples in storage or transportation to a great extent. The transport liquid provided by the invention is simple to prepare, can effectively improvethe activity of a fresh tumor sample, can well protect the cellular morphology of the tumor sample, can significantly increase the modeling rate of PDX, shortens the modeling time, has great help forestablishment of a PDX tumor model and has a high commercial application value.

The invention discloses a construction method of an Adriamycin nephropathy animal model, for solving the technical problem that an existing Adriamycin nephropathy animal model is complex in operation and low in success rate. The construction comprises the following steps: selecting rats according with experimental standards as a molding group; injecting Adriamycin to the rats of the molding group by caudal vein for the first time according to a using amount of 4mg / kg; one week later, injecting Adriamycin for the second time according to the using amount of 3.5mg / kg; from the first injection of Adriamycin to the rats in the molding group, feeding the rats with high protein feed for two weeks, and then feeding with a normal feed for 6 days; and from the first injection of the Adriamycin to the 20th day, reckoning the rats with the total amount of 24-hour urine protein being larger than 30mg into the Adriamycin nephropathy animal model. The construction method disclosed by the invention is simple, feasible, high in success rate, good in consistency and stability and capable of effectively reducing the animal death rate and is a relatively ideal animal model for further researching sertoli cytopathy.

The invention discloses a molding method of a rhesus monkey pulmonary fibrosis model, a preparation, a preparation method of the preparation, the rhesus monkey pulmonary fibrosis model and the application thereof. The molding method comprises the following steps: S1, performing subcutaneous injection of paraquat solution on rhesus monkey, and dripping bleomycin solution to trachea of rhesus monkey; S2, generating the rhesus monkey pulmonary fibrosis model. After molding is performed, the fact whether rhesus monkey molding is successful is diagnosed and the efficacy of a drug for treating pulmonary fibrosis is evaluated through the observation of the clinical symptoms, autonomic activities, feed intake, respiratory rate, haematological index, color dopplar ultrasound and lung tissue pathological results of rhesus monkey. An animal model built through the method, clinical symptoms, haematological indexes, color dopplar ultrasound and lung tissue pathological alteration are similar to lung fibrosis symptoms, moreover, compared with a model built by independently injecting paraquat or independently dripping bleomycin, a model of paraquat and bleomycin is higher in success rate and better in stability, and is applicable to the valuation of a new drug for treating lung fibrosis.

The invention provides a method for constructing a diabetic nephropathy animal model.The influence of environmental pollution on disease occurrence is simulated, cadmium chloride serves as an inductor of the diabetic nephropathy animal model, administration is mainly achieved through intraperitoneal injection, high glucose and high fat are combined for feeding for 8 weeks, the physiological and biochemical index detection result shows that cadmium chloride can cause rise of blood glucose and increase of the NAG content, and pathological examinations meet the pathological change of diabetic nephropathy.The method has the advantages of being easy to operate, economical, high in model making success rate and the like, the pathogenic process of diabetic nephropathy can be simulated, and the problem that an existing diabetic nephropathy animal model is single is solved.

The invention belongs to the technical field of experimental animal models, and provides a method for constructing a rat model with pine cone injury, which aims to solve the problems of complex operation, low success rate, long modeling time, low repeatability and the like of the conventional rat model with pine cone injury. The method comprises the following steps of: performing intraperitoneal injection of PCPA of 450 mg / kg on a rat for 2 days continuously, once every day; or performing electrical stimulation on the rat by using a shuttle box, wherein the electrical stimulation voltage is 30V, the current is 0.8A, the stimulation time is 30 seconds, the interval is 30 seconds, the cycle is performed for 60 times, and the operation is performed for 5 days continuously; or performing an intraperitoneal injection PCPA method on the rat on the fourth day by using an electrical stimulation method. Three modeling methods, namely pathological histomorphology, serum detection of the contentof melatonin which is a main synthetic secretory hormone of a pine cone, and animal general situation and behavioral auxiliary method objective evaluation, cause injury of the pine cone of the rat, and provide a good model basis for researching and screening medicaments and mechanisms with protective and therapeutic effects on the pine cone injury; and the method is simple and convenient, the modeling time is short, the repeatability is high, and the index evaluation is simple and reliable.

The invention relates to the technical field of medicine, in particular to a cardiac thrombosis animal model and a construction method thereof. The construction method comprises the following steps: introducing a chlorophosphonate preparation into the abdominal cavity of an animal; after 0.5-2 days, carrying out ligation on the anterior descending branch of the left coronary artery of the animal in the anesthetic state; and after ligation for 0.5-2 days, introducing the chlorophosphonate preparation into the abdominal cavity of the animal again. The mouse myocardial infarction model is prepared by a method of myocardial infarction modeling, injection of a small amount of chlorophosphonate lipidosome before and after an operation and removal of macrophages in a short period, and the success rate of left ventricular thrombus formation of a modeling mouse reaches 90% or above. The method is simple and easy to implement and can be widely popularized and applied to experimental animals of various body types, the left ventricular thrombosis forming rate is high, the effect is stable, the modeling time is short, and the cardiac thrombosis animal model is closer to the pathophysiological state of left ventricular thrombosis after myocardial infarction clinically.

The embodiment of the invention discloses an industrial control method, device and system based on reinforcement learning and electronic equipment. The method comprises the steps of obtaining current operation data of industrial equipment; target control information is determined based on a target control decision model corresponding to the industrial equipment and the current operation data, and the target control decision model is obtained by performing reinforcement learning on a preset control decision model based on a target virtual environment model corresponding to the industrial equipment in advance; the target virtual environment model corresponding to the industrial equipment is obtained by performing environment modeling based on historical operation data of the industrial equipment; and sending the target control information to the industrial equipment, so that the industrial equipment operates based on the target control information. According to the technical scheme of the embodiment of the invention, the accuracy and efficiency of industrial control can be effectively ensured.

The invention discloses a medicinal composition for establishing a hyperuricemia animal model and an application thereof. The medicinal composition comprises ethambutol and uric acid, and can be used for preparing an acute hyperuricemia model with short modeling time, stable model and high modeling success rate. The medicinal composition is administrated to a rhesus monkey in an intragastric manner, a stable and reliable acute hyperuricemia model can be quickly obtained, and the modeling method is reasonable and close to the pathogenesis of human beings.

The invention belongs to the field of animal model construction, and specifically discloses a method for establishing an animal model for repairing muscle tissue damage, comprising the following steps: first anesthetizing the model animal, fixing it in a prone position, and disinfecting the hind limbs of the anesthetized experimental rat; Then, the skin and subcutaneous tissue of the sterilized hindlimb were incised sequentially through surgery to expose the gastrocnemius muscle and separate the lower part of the gastrocnemius muscle; finally, an electrode was used to clamp the gastrocnemius muscle on the exposed side, and a pulsed electric field was applied to the gastrocnemius muscle to establish an animal model of muscle tissue damage repair. . The present invention uses irreversible electroporation to establish an animal model of muscle tissue damage repair, which can provide a technical method that is simple, easy to implement, low in cost, short in modeling time, stable in model, accurate in damage, and accurate in scope and controllable, and can meet the requirements of destroying muscle cells at the same time. Requirement to preserve extracellular matrix structure.