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CKD (chronic kidney disease) animal model establishment

A chronic kidney disease and animal model technology, applied in the field of animal model establishment, can solve the problems of less experimental research, prolonged experimental research time, animal death, etc.

Inactive Publication Date: 2014-10-15
樊均明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, whether the animal models of chronic renal failure are prepared from drugs, surgical procedures or a combination of the two, the modeling techniques have been continuously improved. The direction and experimental research of each system are relatively less developed, and the criteria for diagnosis and efficacy are vaguely defined. Defining CRF animal models based on the stages of human chronic renal failure has certain limitations, because there are certain differences between humans and animals after all, and model animals Lack of good tolerance to more severe uremia, usually prolonging the time limit of experimental research, will lead to death of animals

Method used

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  • CKD (chronic kidney disease) animal model establishment
  • CKD (chronic kidney disease) animal model establishment
  • CKD (chronic kidney disease) animal model establishment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 Preparation of animal model of the present invention

[0039] 1. Experimental animals: provided by the SPF Experimental Research Center of Luzhou Medical College. License: SYXK (Sichuan) 2013-065

[0040] All animals were kept in an SPF-grade environment with half light and half light, room temperature 22°C-24°C, and humidity 50%-70%. Animals were fed full-price pellets and had free access to food and water.

[0041] 2. Research design: grouped according to dose, given drug intervention for 4 weeks, observed for 12 weeks, observed the characteristics of the occurrence and development of different pathological lesions in model rats, collected specimens, and detected related indicators.

[0042]3. Intervention method: According to different groups, different doses of doxorubicin were injected into the tail vein at one time, and adenine was given by intragastric administration every day.

[0043] 4. Experimental process:

[0044] 140 SD rats were divided i...

Embodiment 2

[0113] Example 2 Use the drug for the treatment of chronic kidney disease to verify the chronic kidney disease animal model of the present invention and preliminarily establish a relevant drug screening platform

[0114] An animal model of chronic kidney disease was prepared according to the method in Example 1; benazepril was selected to treat the model, and the specification was 5 mg / tablet. Make a solution with a mass concentration of 0.2 g / L with physiological saline.

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Abstract

The invention relates to an application of adriamycin and adenine jointly used in preparing animal models used for screening medicine for treating a CKD. According to the invention, the application dose of adriamycin is 2.5-10 mg / Kg each time, and the application dose of adenine is 200 mg / Kg every day. The animal model more accords with the occurrence and development process of the human CKD, experimental results prompt that the model making time can be shortened when the two kinds of medicine are applied jointly, and kidney injury is more serious at the same time point.

Description

technical field [0001] The present invention relates to the establishment of an animal model of chronic kidney disease, specifically, the establishment of an animal model of chronic kidney disease accompanied by compound damage of glomeruli and tubulointerstitium. Background technique [0002] Chronic kidney disease (CKD) is the vast majority of kidney diseases (such as glomerulonephritis, occult nephritis, pyelonephritis, allergic purpura nephritis, lupus erythematosus nephritis, gouty kidney, IgA nephropathy, nephrotic syndrome, The clinical collective name of membranous nephropathy, diabetic nephropathy, hypertensive nephropathy, polycystic kidney disease); except for acute nephritis and acute urinary tract infection (acute inflammatory disease of the kidney), they can all belong to the scope of chronic kidney disease; this disease is a A relatively benign disease (or a common clinical pathological process) that develops slowly; however, if it is not timely and effectivel...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61K49/00A61P13/12A61K31/52
Inventor 樊均明芶芳芳马欣
Owner 樊均明
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