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Feed for preparation of alcoholic liver animal model

An alcoholic liver disease and animal model technology is applied in the field of feed for preparing alcoholic liver disease animal models. High mold success rate, easy to popularize and apply

Active Publication Date: 2016-08-31
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the model of alcoholic hepatitis and liver fibrosis can be successfully constructed, it does not conform to the drinking method of clinical alcoholic liver disease patients, and the modeling time is long, the modeling operation is complicated, the success rate of the operation is low, and the animal mortality rate is high
The Tipoe-Nanji alcohol liquid diet uses fish oil as the main fat, and the mice eat freely. The established female animal model not only has fatty liver, but also is accompanied by inflammation, necrosis and even mild liver fibrosis, but it cannot be seen in the male animal model. fibrosis

Method used

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  • Feed for preparation of alcoholic liver animal model
  • Feed for preparation of alcoholic liver animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061]A kind of feed that is used to prepare animal model of alcoholic liver disease, taking preparation 1L described feed as example, its preparation method is as follows:

[0062] 1) 41.4 grams of casein, 0.5 grams of L-cystine, 0.3 grams of DL-methionine, 10 grams of dietary cellulose, 3.0 grams of xanthan gum, 0.53 grams of choline bitartrate, 8.75 grams of mineral premix, vitamin Add 2.5 grams of premix, 2.0 grams of cholesterol and 0.2 grams of sodium cholate into a container, then add an appropriate amount of water, and stir evenly to obtain mixture A;

[0063] 2) Add 12.8-25.6 grams of maltodextrin into another container, then add an appropriate amount of water, stir evenly, add it to mixture A, stir evenly, bottle it, seal it, and store it at 4°C for later use.

[0064] 3) Add 30-40 grams of fish oil and 25-50 grams of alcohol (alcohol is measured by ethanol, and the specific form of alcohol is liquor) into the bottle of step 2), and stir evenly to obtain 1 L of feed ...

Embodiment 2

[0066] A kind of feed that is used to prepare animal model of alcoholic liver disease, taking preparation 1L described feed as example, its preparation method is as follows:

[0067] 1) 41.4 grams of casein, 0.5 grams of L-cystine, 0.3 grams of DL-methionine, 10 grams of dietary cellulose (Roquette water-soluble dietary fiber, French Roquette), 3.0 grams of xanthan gum, and heavy tartaric acid Choline 0.53 g, Mineral Premix (Dyets.Inc#210011) 8.75 g, Vitamin Premix (Dyets.Inc#310011) ( http: / / dyets.com / vitamin-mixes / ) 2.5 grams, 2.0 grams of cholesterol and 0.2 grams of sodium cholate were added to the container, then an appropriate amount of water was added, and the mixture was evenly stirred to obtain mixture A;

[0068] 2) Add 25.6 g of maltodextrin into another container, then add an appropriate amount of water, stir evenly, add it to mixture A, stir evenly, bottle it, seal it, and store it at 4°C for later use.

[0069] 3) Add 36g fish oil (DSM#MEG-3, containing 12%-13...

Embodiment 3

[0071] Example 3 Preparation of Alcoholic Liver Disease Animal Model

[0072] Male Kunming mice were divided into 2 groups: 15 Kunming mice in the model group and 6 Kunming mice in the pair-feeding group. Each mouse was reared in a single cage, and the feed prepared in Example 2 was freely eaten every day. The feed for mice in the pair-feeding group was formulated with maltodextrin instead of alcohol in the above-mentioned feed. From the second week of feeding, the model group was given alcohol once every 5-7 days (56°Red Star Erguotou, supervised by Beijing Red Star Co., Ltd.), the initial dose was 4g / kg, and then gradually increased to 5g / kg each time. kg, 6g / kg, 6g / kg. After the fourth gavage, a liver was selected from an animal, fixed in 10% formalin, stained by H&E and Masson, and observed histopathologically.

[0073] Wherein, the gavage dosage of alcohol is all measured with ethanol, the following examples are the same.

[0074] A large number of liver cell necrosis...

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Abstract

The invention belongs to the technical field of medical animal model feed and discloses feed for preparation of an alcoholic liver animal model. The feed for mice is prepared from casein, L-cystine, DL-methionine, maltodextrin, meal cellulose, xanthan gum, choline bitartrate, a mineral premix, a vitamin premix, cholesterol, sodium cholate, fish oil, alcohol and water. The feed for preparation of an alcoholic liver animal model can gradually cause mouse alcoholic fatty liver, hepatitis and liver fibrosis. The model has features similar to human diseases and pathology has a certain development process. A modeling method is simple and easy and has a high success rate, a low death rate, good repeatability and a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of medical animal model feed, in particular to a feed for preparing animal models of alcoholic liver disease. Background technique [0002] Alcoholic liver disease is a liver disease caused by long-term excessive drinking. Its initial manifestation is alcoholic fatty liver, and then develops into alcoholic hepatitis, liver fibrosis and even liver cancer, and finally it cannot be cured. Alcoholic liver disease is the most common liver disease in western countries. With the development of economy in my country, its incidence is increasing, seriously affecting the quality of life of patients, and causing a heavy economic burden to families and society. The pathogenesis of alcoholic liver disease is very complicated, and there are few drugs for the treatment of alcoholic liver disease. It is imminent to study an effective drug for the treatment of alcoholic liver disease, and the screening of drugs relies on an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/50A23K20/105A23K20/142A23K20/147A23K20/158A23K20/163A23K20/168A23K20/174A23K20/20A01K67/02
Inventor 李小军穆云妹唐和斌李玉桑曾紫璇邓明华
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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