The invention belongs to the field of microbes and relates to a MEM cell culture medium. Each liter of the MEM cell culture medium comprises 200.00mg of anhydrous calcium chloride, 93.50mg of anhydrous magnesium sulfate, 400.00mg of potassium chloride, 6800.00mg of sodium chloride, 115.00mg of anhydrous sodium dihydrogen phosphate, 126.00mg of L-arginine hydrochloride, 31.40mg of L-cystine hydrochloride, 42.00mg of L-histidine hydrochloride, 52.00mg of L-isoleucine, 52.00mg of L-leucine, 73.00mg of L-lysine hydrochloride, 15.00mg of L-methionine, 36.00mg of L-tyrosine, 40.00mg of L-valine, 18.00mg of choline bitartrate, 3.00mg of folic acid, 2.00mg of inositol, 1.00mg of nicotinamide, 3.00mg of D-calcium pantothenate, 1.00mg of pyridoxine hydrochloride, 0.50mg of riboflavin, 2.00mg of thiamine hydrochloride, 1000.00mg of glucose, 75.00mg of succinic acid, 32.00mg of L-phenylalanine and 60.00mg of kanamycin. The MEM cell culture medium has the advantages of simple formula, convenient preparation and operation, low cost, popularization feasibility, fast cell growth, difficult pollution and low variation rate.