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A method for establishing a hyperlipidemia rat model in a short period of time

A rat model and hyperlipidemia technology, applied in the field of experimental animals, can solve the problems of decreased serum total cholesterol volatility, long cycle and high cost, and achieve the effects of short cycle, good reproducibility and low cost

Active Publication Date: 2019-02-05
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, it generally takes 6-8 weeks to raise a rat model of hyperlipidemia by feeding it with high-fat feed, which is too long and relatively expensive; Unstable conditions such as decreased volatility of serum total cholesterol

Method used

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  • A method for establishing a hyperlipidemia rat model in a short period of time
  • A method for establishing a hyperlipidemia rat model in a short period of time
  • A method for establishing a hyperlipidemia rat model in a short period of time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 Determination of growth performance of rats

[0031] During the feeding period, the rats in each group were weighed every day, the average weight of the rats in each group was calculated and recorded, the body weight growth curve was drawn, and the percentage of body weight gain of the rats during the feeding period was calculated. During the whole test period (21 days), with the prolongation of feeding time, the body weight of the rats in the control group and the model group showed an increasing trend; during the 1st to 14th day of the experiment, the weight of the rats in the two groups maintained a relatively consistent increase speed; but from the 14th day, the weight of rats in the control group continued to grow at the original speed; while the weight growth of rats in the model group began to slow down ( figure 1 ). According to the average body weight of the rats during the whole test period, on the 1st, 10th, and 14th days of the test, there was ...

Embodiment 2

[0034] Example 2 Detection of Total Cholesterol Concentration in Rat Serum

[0035] During the feeding period, the blood of each group of rats was collected from the retro-orbital venous plexus on the 10th, 14th, and 21st days of feeding, and after standing for coagulation, centrifuged at 3000-5000r / min for 10min, the upper serum was taken and placed in Another centrifuge tube was frozen and stored for testing; according to the instruction manual of total cholesterol in serum, the absorbance of each serum sample was detected on an automatic biochemical analyzer by biochemical method, and the concentration of total cholesterol in the sample was calculated according to the formula. Depend on figure 2 It can be seen that in the rat serum taken on the 10th day, the 14th day and the 21st day, the total cholesterol concentration in the rat serum of the model group was significantly higher than that of the control group; The concentration of total cholesterol in the serum of the ra...

Embodiment 3

[0036] Example 3 Detection of Triglyceride Concentration in Rat Serum

[0037] According to the instructions of the serum triglyceride determination kit, the absorbance of each serum sample was detected on an automatic biochemical analyzer by a biochemical method, and the concentration of triglyceride in the sample was calculated according to the formula. Depend on image 3 It can be seen that in the rat serum taken on the 10th day, the 14th day and the 21st day, the triglyceride concentration in the model group rat serum was significantly higher than that of the control group; on the 10th day, the 14th day and the 21st day, The concentration of triglycerides in the serum of rats in the model group was 80%, 81% and 98% higher than that in the control group.

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Abstract

The invention discloses a method for establishing a hyperlipidaemia rat model in a short term. Animals are male Sprague Dawley rats, the body weight of the rats is 150+ / -20 grams. A high-fat diet formula includes 23.31% of casein, 0.35% of L-cystine, 8.48% of corn starch, 11.65% of maltodextrin, 20.17% of saccharose, 5.83% of cellulose, 2.91% of soybean oil, 20.68% of lard oil, 5.23% of compound mineral substances, 1.16% of compound vitamin, and 0.23% of Choline bitartrate. A fat emulsion formula includes 20% of Tween 80, 20% of lard oil, 10% of cholesterol, 2% of sodium cholate, 20% of propylene glycol, 20% of glucose, and 1% of propylthiouracil. The operation method includes: constinuously feeding rats with the high-fat diet for 21 days, and performing self-feeding; feeding the rats with the fat emulsion prepared according to the formula through the gavage method, wherein the dosage is 2 ml / kg. day. The method is short in establishment cycle of the rat hyperlipidaemia animal model, is low in cost, is good in reproducibility, can effectively simulate the human incidence state. Accordingly, the method can be used for research on functional foods capable of preventing hyperlipidaemia and screening of lipid-lowering drugs.

Description

technical field [0001] The invention belongs to the technical field of experimental animals, and in particular relates to a method for making rats have the pathological characteristics of hyperlipidemia in a short period of time; the model can be used for the research of functional foods for preventing hyperlipidemia, and can also be used for the screening of blood lipid-lowering drugs , drug efficacy evaluation and pharmacological research and other fields. Background technique [0002] With the change of people's diet structure, the incidence of hyperlipidemia is increasing year by year. Hyperlipidemia is a systemic disease with abnormal lipid metabolism or operation, that is, the increase of serum total cholesterol, triglyceride, low-density lipoprotein and the decrease of high-density lipoprotein. One of the main causes of atherosclerosis, heart disease, stroke and fatty liver, a serious threat to human health. Finding safe and effective blood lipid-lowering drugs has ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 韩菲菲韩剑众范寒雪刘芳
Owner ZHEJIANG GONGSHANG UNIVERSITY
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