190 results about "High oleic" patented technology

This invention relates to corn (Zea mays L.) seed and grain having a significantly higher oleic acid content than conventional corn by virtue of heritable genes for increased oil and oleic acid content and / or lowered levels of linoleic acid. The present invention also relates to the production of high oil, high oleic grain, its oil, its progeny and its use.

The present invention provides methods of marker assisted selection for high oleic / low linolenic traits in canola and in other oil seed crop species, as well as isolated nucleic acids for use as molecular markers in such methods. In particular, molecular markers and Brassica nucleic acid corresponding to fad2 and fad3 gene mutations are disclosed. The markers of the present invention are highly useful for the direct selection of desirable fad2 and fad3 alleles during marker-assisted trait introgression and breeding. In a one aspect of the embodiment, two single nucleotide polymorphism (SNP) markers are provided which correspond to the alleles. Thus, the present invention advantageously permits one of skill in the art to breed for the molecular markers described herein, or derivatives thereof, rather than breeding for a high oleic / low linolenic phenotype.

In various aspects, the invention provides Brassica juncea plants, seeds, cells, nucleic acid sequences and oils. Edible oil derived from plants of the invention may have significantly higher oleic acid content than other B. juncea plants. In one embodiment, the B. juncea line MJ02-086-3 contains a mutant allele MJ02-086-3 / BjFAD2-a at the BjFAD2-a gene locus, having a premature stop codon, so that there are no functional copies of the FAD2 gene in MJ02-086-3 plants. Seeds from MJ02-086-3 plants may for example yield an oil having oleic acid content of greater than about 70% by weight.

A method of treating an earth formation that includes injecting at least one lipophilic monomer into the earthen formation; wherein the at least one lipophilic monomer is selected from epoxide-functionalized derivative of soybean oil linseed oil, rapeseed oil, cashew nut shell oil; perilla oil, tung oil, oiticia oil, safflower oil, poppy oil, hemp oil, cottonseed oil, sunflower oil, high-oleic triglycerides, triglycerides of euphorbia plants, peanut oil, olive oil, olive kernel oil, almond oil, kapok oil, hazelnut oil, apricot kernel oil, beechnut oil, lupine oil, maize oil, sesame oil, grapeseed oil, lallemantia oil, castor oil, herring oil, sardine oil, menhaden oil, whale oil, and tall oil, injecting at least one crosslinking agent into the earthen formation; wherein the at least one crosslinking agent comprises at least one primary amine; and allowing the lipophilic monomer and the crosslinking agent to react in the earth formation is disclosed.

[Problem]Providing soybean including oleic acid that will exceed the heretofore developed high-oleic soybean without using gene engineering technology.[Means to Solve the Problem]The present invention provides a GmFAD2-1b gene loss of function mutation and a type of soybean that includes a GmGAD2-1b gene loss of function mutation.

Butters are made from high oleic vegetable oils during a procedure by which a large percentage of the oleic acid is transformed into trans-configured elaidic acid, without significantly increasing the saturated fat present in the high oleic vegetable oil. The vegetable oils have an initial oleic acid content of at least about 75 weight percent, with the hard butter made from it being a high elaidic hard fat having at least about 65% trans-configured elaidic acid. The preferred process is a single-step procedure of hydrogenation in the presence of a deadened catalyst such as a sulfur-poisoned nickel catalyst.

In various aspects, the invention provides Brassica juncea plants, seeds, cells, nucleic acid sequences and oils. Edible oil derived from plants of the invention may have significantly higher oleic acid content than other B. juncea plants. In one embodiment, the B. juncea line MJ02-357-3 contains a mutant allele MJ02-313-1 / BjFAD2-a at the BjFAD2-a gene locus, having a single base-pair change (a G to A substitution in the ORF at position 281 in reference to the first ATG start codon) relative to the wild type sequence. The change is predicted to encode a Glycine-94 Aspartic acid mutation in the sequence of the predicted BjFAD2-a protein. In another embodiment, the B. juncea line MJ02-357-3 contains a mutant allele MJ02-357-3 / BjFAD2-a at the BjFAD2-a gene locus, having a single base-pair change (a C to T substitution in the ORF at position 647 in reference to the first ATG start codon) relative to the wild type sequence. The change is predicted to encode a Proline-216 Leucine mutation in the sequence of the predicted BjFAD2-a protein. As a result of these mutations, it can be predicted that the function of the BjFAD2-a proteins are negatively affected in Brassica juncea lines MJ02-313-1 and MJ357-3 as reflected in the increased levels of oleic acid in seed oil in comparison with the wild-type line J96D-4830. Seeds from MJ02-313-1 and MJ02-357-3 plants may for example yield an oil having oleic acid content of greater than 70% by weight.

The invention discloses a peanut high-oleic-acid character gene selection method. The method comprises the steps of extracting leaf gene groups of different peanut breeds, and amplifying AhFAD2A and AhFAD2B genes from the gene groups by using a specificity primer; cutting and recycling specificity amplification stripes and sequencing to obtain the sequence of the gene; translating the obtained sequence, comparing and analyzing, and searching the point mutation or inserting site to judge the oleic acid content and specific value of oleic acid / linoleic acid (O / L) of the breed. The method is simple to operate, variety of peanuts germ plasm can be analyzed, the oleic acid content of single plant of peanut can be determined in the seedling stage, the breeding process can be shortened and the breeding effect of oleic acid can be improved by combining comprehensive agronomic character selection of the single plant.

The invention discloses a method for breeding high-yield high-oleic-acid high-oil peanuts, which comprises the following steps: crossing a high-yield high-oleic-acid peanut variety and a high-yield high-oil peanut variety; backcrossing the hybrid generations with the parent generations respectively; continuing to backcross high-oleic-acid single plants from AC1F1 generation and high-oil single plants from BC1F1 respectively to obtain AC2F1 and BC2F1 respectively; performing selfing of the AC2F1 and selfing of BC2F1 respectively; after selfing, performing crossing to obtain secondary crossing generation; performing continuous multi-generation selfing by starting from the secondary crossing generation, selecting high-yield high-oleic-acid high-oil single plants for selfing propagation from each generation after the F2 generation and while selecting high-yield single plants, performing high-oleic-acid and high-oil nondestructive selection by using nondestructive selection technology, andperforming the selfing of the selected high-yield high-oleic-acid high-oil single plants; and performing comparative selection of lines from F6 generation and obtaining new high-yield high-oleic-acidhigh-oil peanut variety.

The invention relates to a high-oleic-acid-content molecular marker, a back cross breeding method for carrying out assistant selection by utilizing the molecular marker, and an application of the back cross breeding method. Sequences of variant specific primers of the molecular marker are as follows: a forward primer MITE-INS-F, and a reverse primer MITE-INS-R; the sequences of non-variant specific primers are as follows: a forward primer WILD-F, and a reverse primer WILD-R. The back cross breeding method utilizing the molecular maker comprises the following steps: carrying out hybridization on a peanut parent of a gene type AABB and a parent of a gene type aabb, then carrying out cross breeding; amplifying an FAD2A gene segment by utilizing one pair of primers in a genome, and selecting a single plant containing a genotype Aa; carrying out PCR (polymerase chain reaction) detection by utilizing a primer, at a site ahFAD2B, of a peanut as a marker, and selecting a single plant containing a genotype Bb; carrying out back crossing on the single plant which is dominant in two markers, namely the single plant containing the genotype AaBb, and the parent AABB until a high-oleic-acid-content single plant which is of a genotype aabb and is consistent with a recurrent parent in other economical characters is selected from later generations, and then carrying out self-crossing and amplifying propagation. The high-oleic-acid-content molecular marker has the advantages of being simple in method, low in cost and stable in result; when the high-oleic-acid-content molecular marker is used for carrying out the back cross breeding, the accuracy of character selection can be obviously improved, the breeding cost is reduced, and the breeding efficiency is improved.

The invention provides ketogenic nutrient meal replacement powder. The ketogenic nutrient meal replacement powder is prepared from the following components: medium chain triglyceride microcapsule powder, gamma-linolenic acid oil microcapsule powder, high oleic sunflower oil microcapsule powder, rice bran fatty alkanol microcapsule powder, oat fiber, soy protein isolate, rice protein, yeast protein, psyllium seed husk powder, a potato extract, an agaricus bisporus extract, a white kidney bean extract, erythritol, grape fruit powder, compound vitamins, compound minerals and xanthan gum. The formula contains high-content fat, contains dietary fiber, various vitamins and minerals, can increase satiety, control the decomposition and absorption of carbohydrates, avoid fat accumulation, and can effectively improve the unwell symptoms of a user when glucose energy supply is converted into ketone body energy to achieve nutrient balance, and can be used as a ketogenic diet for weight loss peopleand epilepsy patients.

The present invention relates to extracted lipid with high levels, for example 90% to 95% by weight, oleic acid. The present invention also provides genetically modified plants, particularly oilseeds such as safflower, which can used to produce the lipid. Furthermore, provided are methods for genotyping and selecting plants which can be used to produce the lipid.

A novel method for improving the meat, milk, and egg quality of livestock is provided. In one embodiment, the method comprises feeding the animal a diet supplemented with oleic acid and distillers grains. The source of the oleic acid may be distillers grain from high-oleic corn. The method improves the quality of meat from both non-ruminants and ruminants.

The invention belongs to the field of peanut oil, and discloses a high-oleic-acid peanut oil which is processed with high-oleic-acid peanuts as the raw material through the process of stone screening, impurity removal achieved through winnowing, color sorting and seeking, rotary baking, appropriate-temperature squeezing, low-temperature filtering and the like. By means of the process, the safety quality and nutritional quality of the high-oleic-acid peanut oil are ensured, the oleic acid content in the produced high-oleic-acid peanut oil product ranges from 50% to 82%, and the high-oleic-acid peanut oil is comprehensive in nutrient, has the inherent smell and flavor of peanut oil, and is free of peculiar smell, suitable for frying, not prone to oxidization and long in shelf life.

The invention discloses environment-friendly type transformer oil with a high ignition point and a preparation method of the transformer oil and belongs to the technical field of lubricating oil. The environment-friendly type transformer oil with the high ignition point comprises components in percentage by weight as follows: 99.5%-99.8% of high-oleic rapeseed oil, 0.1%-0.4% of an antioxidant and 0.1%-0.4% of a metal deactivator. The invention further provides a preparation method of the transformer oil. The preparation method comprises steps as follows: the high-oleic rapeseed oil is heated to 50-80 DEG C, the antioxidant and the metal deactivator are added sequentially, the materials are stirred and mixed for 1-5 h, and the transformer oil is obtained after cooling and refined filtration. The transformer oil adopts a simple process, does not contain harmful substances, is biodegradable and cannot damage the environment and the human body; the performance is stable, good stability is kept between different batches, the ignition point is high, and risks of fire disasters and explosions are effectively reduced; the addition quantity of the antioxidant is low, the service period is long, and the transformer oil has excellent physicochemical and dielectric properties and meets the requirement of national standard.

A novel method for improving the meat quality of an animal is provided. In one embodiment, the method comprises feeding the animal a diet supplemented with oleic acid and tocols and subsequently formulating the meat with a non-tocol antioxidant such as rosemary extract. The source of the oleic acid and / or tocols may be transgenic corn that employs the FAD-2 gene as a silencing agent for a high-oleic phenotype and / or expresses the HGGT gene for a high-tocotrienol phenotype. The method improves the quality of meat from both non-ruminants and ruminants.

A wellbore fluid that includes an oleaginous continuous phase; a non-oleaginous phase; and a polymeric additive formed by mixing at least one lipophilic monomer and at least one crosslinking agent, wherein the at least one lipophilic monomer is at least one of an epoxide-functionalized derivative of at least one selected from soybean oil, linseed oil, rapeseed oil, cashew nut shell oil; perilla oil, tung oil, oiticia oil, safflower oil, poppy oil, hemp oil, cottonseed oil, sunflower oil, high-oleic triglycerides, triglycerides of euphorbia plants, peanut oil, olive oil, olive kernel oil, almond oil, kapok oil, hazelnut oil, apricot kernel oil, beechnut oil, lupine oil, maize oil, sesame oil, grapeseed oil, lallemantia oil, castor oil, herring oil, sardine oil, menhaden oil, whale oil, tall oil, and synthetic aliphatic or aromatic ethers, and the at least one crosslinking agent includes at least one selected from amines, alcohols, phenols, thiols, carbanions, carboxylates, and mixtures thereof is disclosed.

The invention discloses a method for breeding high-oleic-acid disease-resistance peanuts, which comprises the following steps: obtaining first F1 generation by crossing bacterial wilt resistant peanuts and high-oleic-acid peanuts; backcrossing a crossing F1 generation with high-oleic-acid peanuts to obtain backcrossing first generation BC1F1, backcrossing bacterial wilt resistant plants and high-oleic-acid peanuts from BC1F1 to obtain BC2F1, backcrossing bacterial wilt resistant plants and high-oleic-acid peanuts from BC2F1 to obtain BC3F1, performing selfing of bacterial wilt resistant plants from BC3F1 to obtain BC3F2, crossing plants selected from BC3F2 with a leaf spot resistant variety to obtain a second crossing F1; and performing continuous selfing starting from the second F1 generation to obtain F2, F3, F4 and F5 generations, entering an identification nursery from F6 generation, performing quality identification and obtaining high-oleic-acid disease-resistance peanuts. The peanut variety bred by the method has high oleic acid content and high disease resistance, and therefore is suitable to be planted in areas where peanut diseases occur frequently. The method has remarkable economic and ecological benefit.

The invention relates to plant seeds that contain an oil having an oleic acid content of more than 40 wt % and a stearic acid content of more than 12 wt % based on the total fatty acid content of said oil, and wherein a maximum of 10 wt % of the fatty acid groups in the sn-2 position of the TAG molecules constituting the oil are saturated fatty acid groups. The invention also relates to plants that can be grown from the seeds, oil that can be extracted from the seeds, and to methods for obtaining the seeds, plants and oil.

The present invention relates to a creamer composition comprising ultra-high oleic oils, with an oleic acid content from 85 to 97% by weight of the total fatty acids in the creamer composition. The creamer composition may comprise blend of high oleic sunflower and high oleic soybean oil in the range of 90:10 to 10:90 or blend of high oleic soybean and high oleic algal oil in the range of 90:10 to 10:90. The creamer composition may also comprise added oil soluble antioxidants. The invention also relates to a beverage composition comprising the liquid or powder creamer composition, and a method of making it.

Soy protein products obtained from high oleic soybeans, wherein such products, have improved whiteness, reduced viscosity and reduced gel-strength, are described. Use of such products in food, beverage and animal feed are also disclosed.

The invention relates to the technical field of peanut breeding and provides a rapid and efficient breeding method for a peanut variety with high oleic acid content. According to the method, the oleicacid content of single-grain peanuts is detected by near infrared on the basis of the law of the number of AhFAD2 gene mutations in single-grain peanut seeds and the oleic acid content, the oleic acid content can be selected in the early stage of hybridization by setting different selection conditions of the oleic acid content without dependence on molecular markers, and the peanut variety with the high oleic acid content is rapidly and efficiently bred; the method is rapid, efficient, simple and easy to operate, the breeding progress of the peanut variety with the high oleic acid content iseffectively accelerated, breeding efficiency is improved, and cost is reduced to a great degree.

The invention belongs to the field of rapeseed breeding, and particularly relates to a cultivating method for a high-oleic acid rapeseed variety. The cultivating method is characterized by comprising the following steps: performing hybridization by taking first Brassica napus L. A177 with the oleic acid content of 65 percent or below as a female parent and taking first high-oleic acid Brassica napus L. H003 which has the preservation number of CCTCC NO:P201604 and has the oleic acid content of at least 78 percent as a male parent, and obtaining F1 through artificial emasculation; planting the F1, obtaining a separated DH line through microspores culture; performing high-oleic acid molecular marker identification on strain seedling stage of a DH line group, and determining the genotype of each strain; measuring the content of oleic acid of each single plant by utilizing a gas chromatograph, and determining the content of the oleic acid of the each primarily selected line; selecting the parent strains of which the characters, such as the whole plant pod number, the seed number per pod, the thousand seed weight and the yield per plant, are superior to the parent strain with the oleic acid content lower than 69 percent from the DH strain with the oleic acid content of greater than 75 percent to obtain the high-oleic acid rapeseed selected line and variety of which the oleic acid is greater than 78 percent.

The invention relates to a high-oleic-acid, drought-resistant and barren-resistant peanut variety breeding method, which comprises the following steps of: 1) screening high-oleic-acid drought-resistant individual plants to obtain high-oleic-acid drought-resistant individual plants; 2) screening high-oleic-acid barren-resistant individual plants to obtain high-oleic-acid barren-resistant individual plants; 3) screening high-oleic-acid, drought-resistant and barren-resistant individual plants to obtain high-oleic-acid, drought-resistant and barren-resistant individual plants, and 4) rapidly breeding the high-oleic-acid, drought-resistant and barren-resistant individual plants obtained at the step 3 to obtain a new high-oleic-acid, drought-resistant and barren-resistant peanut variety. Through the steps, the new high-oleic-acid, drought-resistant and barren-resistant peanut variety can be obtained. The breeding method disclosed by the invention has the advantages of simple process, shortbreeding cycle, small blindness and high efficiency.

This invention relates to corn (Zea mays L.) seed and grain having a significantly higher oleic acid content than conventional corn by virtue of heritable genes for increased oil and oleic acid content and / or lowered levels of linoleic acid. The present invention also relates to the production of high oil, high oleic grain, its oil, its progeny and its uses.

A mayonnaise product in which the oil phase includes a combination of medium chain triglyceride (MCT) oil and a saturated fat such as butter oil, coconut oil, lard or palm oil. The MCT oil tends to reduce crystallization of the saturated fat when cooled, and consequently this improves emulsion stability. In another embodiment, the mayonnaise contains ascorbyl palmitate in an amount sufficient to reduce the size of fat crystals when cooled. In another embodiment, the oil phase also contains up to about 40% vegetable oil such as olive oil or high oleic sunflower oil. In another embodiment, the mayonnaise contains whole butter. In this embodiment, the aqueous portion of the whole butter improves emulsion stability.