Tumor tissue transport fluid and application thereof
A technology of tumor tissue and transport fluid, applied in the biological field, can solve the problems of lack of nutrients in tumor cells, reduced success rate of PDX membrane construction, and reduced tissue activity, etc., to achieve high commercial application value, protect cell morphology, and improve the lack of nutrients Effect
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Embodiment 1
[0016] Example 1: Configuration of tumor tissue transport solution
[0017] In the present invention, DMEM high-glucose medium is used as the basic medium for maintaining tissue activity; insulin (Insulin) is used for maintaining the uptake and utilization of glucose by tissue cells; thyrotropin-releasing factor (TRF) is also known as stimulating Thyroid hormone-releasing hormone is used to maintain the activity of tissue cells; cortisol is a type of glucocorticoid used to maintain the metabolism of sugar, lipid and protein in tissue cells, and transferrin is added to carry iron released by red blood cell degradation to maintain cell Metabolism; adding glutamine and non-essential amino acids is mainly to supplement the amino acids needed for tissue cell metabolism; adding sodium selenite to supplement trace elements; adding penicillin, streptomycin, gentamicin, and amphotericin antibiotics are In order to prevent the tissue from being bio-contaminated by bacteria during the tr...
Embodiment 2
[0019] Example 2: Preparation of tumor tissue transport solution
[0020] Take a 50ml sterile centrifuge tube, add 0.5 mg / ml insulin, 1mM TRF, 100unit / ml penicillin, 100μg / ml streptomycin, 50 mg / ml gentamicin, 0.25 mg / ml The tumor tissue transfer solution was prepared by adding ml amphotericin, 0.5% non-essential amino acids, 0.5% L-glutamine, 18 ng / ml cortisol, 0.1 mg / ml transferrin and 0.1 μg / ml sodium selenite.
Embodiment 3
[0021] Example 3: Configuration of tumor tissue transport solution
[0022] Take a 50ml sterile centrifuge tube, add 1 mg / ml insulin, 6 mM TRF, 100unit / ml penicillin, 100μg / ml streptomycin, 50 mg / ml gentamicin, 0.25 mg / ml amphotericin, 2% non-essential amino acids, 2% L-glutamine, 30 ng / ml cortisol, 0.4 mg / ml transferrin and 0.6 μg / ml sodium selenite to prepare tumor tissue transfer solution .
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