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Application of composite high-fat forage to construct non-alcoholic fatty liver disease rat model

A high-fat feed, non-alcoholic technology, applied in the field of medicine, can solve the problems of not being able to truly reflect the pathogenesis of the disease, evaluate prevention and control measures, etc., and achieve the effects of good application prospects, short modeling time and simple formula.

Inactive Publication Date: 2015-04-15
NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, when conventional models are used for research, they often cannot truly reflect the pathogenesis of the disease and objectively evaluate the prevention and control measures.

Method used

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  • Application of composite high-fat forage to construct non-alcoholic fatty liver disease rat model
  • Application of composite high-fat forage to construct non-alcoholic fatty liver disease rat model
  • Application of composite high-fat forage to construct non-alcoholic fatty liver disease rat model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Select healthy male SD rats and randomly divide them into a model group and a blank control group. After one week of adaptive feeding, the rats in the model group were given high-fat feed freely, and at the same time, they were given 500 mg / kg / d sodium valproate normal saline solution by intragastric administration once a day, and were continuously fed for 8 weeks. . Rats in the blank control group were supplied with basal feed, and the same amount of blank normal saline was administered orally every day, once a day, and fed continuously for 8 weeks.

Embodiment 2

[0024] Example 2 Select healthy male SD rats (6-8 weeks old, body weight 180-220 grams) and randomly divide them into a model group and a blank control group. After one week of adaptive feeding, the rats in the model group were given high-fat feed freely, and at the same time, they were given 500 mg / kg / d sodium valproate normal saline solution by intragastric administration once a day, and were continuously fed for 8 weeks. . Rats in the blank control group were supplied with basal feed, and the same amount of blank normal saline was administered orally every day, once a day, and fed continuously for 8 weeks. During the modeling period, the compound high-fat feed fed to the model group rats consisted of the following formula: by mass percentage, 77.5% rat basal feed, 10% eggs, 10% coconut oil, 2% cholesterol, 0.5% bile salt; Based on the weight of rats, sodium valproate was 500 mg / kg / d. After 4 weeks of modeling, the rats developed symptoms of fatty liver, which continued to...

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Abstract

The invention belongs to the field of animal experiment models, and discloses application of a composite high-fat forage to construct a non-alcoholic fatty liver disease rat model. The high-fat forage is composed of the following raw materials in percent by mass: 77.5% of a rat basic forage, 10% of egg, 10% of coconut oil, 2% of cholesterol, 0.5% of bile salt, and 500mg / kg / d of sodium valproate calculated according to the rat weight. After 8 weeks, rats all have typical non-alcoholic fatty live symptoms, and liver has a lot of fat accumulation along with inflammatory cell infiltration. The model establishing time is short, the success rate is high, and the composite high-fat forage is applicable to pathogenesis research induced by high-fat-diet combined medicines with liver-toxicity side effect, screening of related control measures and efficacy evaluation of treatment medicines.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a compound high-fat feed and its application in constructing an animal model of non-alcoholic fatty liver. The animal model can be used for the pathogenesis of non-alcoholic fatty liver induced by dietary compound drugs research and evaluation of preventive measures. Background technique [0002] With the improvement of people's living standards, changes in living habits and diet, and the wide application of imaging technologies such as ultrasound, the incidence of non-alcoholic fatty liver is increasing year by year, and it is showing a trend of younger age. It has become the three major liver diseases that endanger human health. one. The causes of non-alcoholic fatty liver are complex. High-sugar and high-fat diet-induced hyperlipidemia, obesity, and diabetes are considered to be the main risk factors for non-alcoholic fatty liver. [0003] With the advent of a large number ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18
Inventor 储小曼郝海平张丽芳王广基王洪谢昊
Owner NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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