Method for establishing muscle tissue damage repairing animal model

Abstract

The invention belongs to the field of animal model construction, and specifically discloses a method for establishing an animal model for repairing muscle tissue damage, comprising the following steps: first anesthetizing the model animal, fixing it in a prone position, and disinfecting the hind limbs of the anesthetized experimental rat; Then, the skin and subcutaneous tissue of the sterilized hindlimb were incised sequentially through surgery to expose the gastrocnemius muscle and separate the lower part of the gastrocnemius muscle; finally, an electrode was used to clamp the gastrocnemius muscle on the exposed side, and a pulsed electric field was applied to the gastrocnemius muscle to establish an animal model of muscle tissue damage repair. . The present invention uses irreversible electroporation to establish an animal model of muscle tissue damage repair, which can provide a technical method that is simple, easy to implement, low in cost, short in modeling time, stable in model, accurate in damage, and accurate in scope and controllable, and can meet the requirements of destroying muscle cells at the same time. Requirement to preserve extracellular matrix structure.

Description

technical field [0001] The invention relates to the field of animal model construction, in particular to a method for establishing an animal model for muscle tissue damage repair. Background technique [0002] Stem cells in adult individual tissues are mostly in a dormant state under normal conditions. When a series of injuries such as trauma, inflammation, degenerative diseases and other consumptive diseases occur in the body, the tissue can maintain a certain degree of regeneration under pathological conditions or induced by external factors. ability. Muscle tissue has its own precursor cells, that is, muscle satellite cells. The muscle satellite cells attached to the surface of muscle cells have the nature of stem cells. When muscle cells are damaged, muscle satellite cells can proliferate and differentiate, and participate in the repair of muscle cell structure and function. However, the specific molecular mechanism is still unclear. As an important research tool for m...

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Problems solved by technology

[0003] At present, animal models commonly used in muscle tissue engineering include hereditary myopathy models, denervation models, and frozen models, but they have different degrees of shortcomings, such as unstable models, high costs, and inaccurate damage (hereditary myopathy) , Denervation affects regeneration research, long modeling period (denervation model) or difficult to control the extent of damage (freezing model)

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