Molding method of rhesus monkey pulmonary fibrosis model, preparation, preparation method of preparation, rhesus monkey pulmonary fibrosis model and application thereof

Abstract

The invention discloses a molding method of a rhesus monkey pulmonary fibrosis model, a preparation, a preparation method of the preparation, the rhesus monkey pulmonary fibrosis model and the application thereof. The molding method comprises the following steps: S1, performing subcutaneous injection of paraquat solution on rhesus monkey, and dripping bleomycin solution to trachea of rhesus monkey; S2, generating the rhesus monkey pulmonary fibrosis model. After molding is performed, the fact whether rhesus monkey molding is successful is diagnosed and the efficacy of a drug for treating pulmonary fibrosis is evaluated through the observation of the clinical symptoms, autonomic activities, feed intake, respiratory rate, haematological index, color dopplar ultrasound and lung tissue pathological results of rhesus monkey. An animal model built through the method, clinical symptoms, haematological indexes, color dopplar ultrasound and lung tissue pathological alteration are similar to lung fibrosis symptoms, moreover, compared with a model built by independently injecting paraquat or independently dripping bleomycin, a model of paraquat and bleomycin is higher in success rate and better in stability, and is applicable to the valuation of a new drug for treating lung fibrosis.

Description

technical field [0001] The invention relates to a modeling method of a rhesus monkey pulmonary fibrosis model, a preparation, a preparation method of the preparation, a rhesus monkey pulmonary fibrosis model and an application thereof, belonging to the field of biomedicine. Background technique [0002] Pulmonary fibrosis generally refers to the pulmonary inflammatory response caused by different factors such as poison, spontaneous immune response, drug side effect, infection, severe trauma, etc., with alveolar surface epithelial cell damage, inflammatory cell accumulation, fibroblast proliferation, extracellular Matrix deposition and scar formation are the main features, and eventually lead to structural changes and loss of function of normal lung tissue, which is the final outcome of interstitial lung disease. The pathogenesis of the disease is unknown, and there is no effective drug treatment at present, and its prognosis is poor, seriously affecting human health and qual...

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Problems solved by technology

[0003] (1) The above animals are not suitable for imaging examination (color Doppler ultrasound examination or X-ray examination) because of their small size, and the success of modeling can only be judged by dissecting the lungs for histopathological examination

[0004] (2) The genes, species, hematological parameters, and lung physiological structure of the above animals are quite different from those of humans, so the experimental data obtained after modeling cannot be directly compared with the data of clinical pulmonary fibrosis patients

Of course, the pharmacodynamic or toxicological results of new drugs for the treatment of pulmonary fibrosis evaluated using these animal models cannot be directly extrapolated to humans.

[0005] (3) The treatment of pulmonary fibrosis is a long process, and the lifespan of mice and rats is generally relatively short, so it may not be possible to see the efficacy of new drugs for the treatment of pulmonary fibrosis within the effective life span of mice and mice

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