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Identification of cotton fiber specificity promoter GhFLA1

A specific, cotton fiber-based technology, applied in the field of cotton fiber-specific promoters, can solve the problems of poor cotton fiber quality and inability to use raw materials for production

Inactive Publication Date: 2010-09-01
HUAZHONG NORMAL UNIV
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Problems solved by technology

my country's cotton output ranks first in the world, but the quality of cotton fiber is poor and cannot be used as raw materials for some high-value-added high-quality textile products

Method used

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  • Identification of cotton fiber specificity promoter GhFLA1
  • Identification of cotton fiber specificity promoter GhFLA1
  • Identification of cotton fiber specificity promoter GhFLA1

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Experimental program
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Embodiment Construction

[0023] Isolation, identification and expression activity analysis of a novel cotton FLA1 promoter and its gene sequence.

[0024] 1. Isolation and identification of cotton FLA1 gene sequence

[0025] More than 10,000 cotton cDNA clones were isolated from the cotton fiber cDNA library. Through bioinformatics analysis, four GhFLA cDNA sequences were identified, one of which was named GhFLA1, and primers GhFLA1Fup and GhFLA1Fdn were designed based on the full-length sequence of GhFLA1 cDNA. The full length of the gene was obtained by PCR amplification.

[0026] 2. Real-time fluorescence quantitative RT-PCR analysis of GhFLA1 gene expression

[0027] (1) Extraction of total tissue RNA (according to the method of Li XB, Cai L, Cheng NH, Liu JW, Molecular characterization of the cotton GhTUB1 gene that preferentially expressed in fiber. Plant Physiol. 2002, 130: 666-674).

[0028] (2) Real-time fluorescent quantitative RT-PCR study of gene expression (according to Li XB, Fan XP, W...

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Abstract

The invention discloses a cotton fiber specificity promoter GhFLA1 and a gene complete sequence thereof, relating to an important regulatory element related to cotton fiber development. The FLA1 gene complete sequence has totally 1920bp and comprises a 5' end promoter and a gene coding sequence. The gene does not contain an intron and encodes an arabinogalactan protein (AGP). The cotton fiber contains a great amount of the AGP protein, and that AGP irreversible inhibitor can inhibit fiber elongation shows that the protein has an important role for cotton fiber development. That the FLA1 gene mRNA has a great amount of specificity accumulation in the process of cotton fiber cell development shows that the gene promoter plays an important role in the regulation of fiber specificity expression. Experiments also show that the promoter has strong expression activity in tobacco leaf epidermal hair, which further proves that the promoter is the specificity regulatory element of the cotton fiber. The promoter can be used as a gene cis-regulatory element to be applied to cotton molecular breeding research so as to improve the cotton fiber quality and to enhance the cotton yield.

Description

Technical field: [0001] The present invention relates to a cotton fiber specific promoter. Specifically, it is the cloning identification and activity analysis of the promoter sequence of a fiber-specifically expressed gene GhFLA1 encoding fasciclin-like arabinogalactan protein (fasciclin-like arabinogalactan protein). Background technique: [0002] Cotton fiber is an important raw material for my country's textile industry and related industries. With the rapid development of the textile industry and the continuous improvement of people's living standards, the textile industry and its related industries have higher and higher requirements for the quality of cotton fibers. my country's cotton output ranks first in the world, but the quality of cotton fiber is poor and cannot be used as raw materials for the production of some high-value-added high-quality textile products. Therefore, my country needs to import more than 1 million tons of high-quality cotton every year, res...

Claims

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Application Information

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IPC IPC(8): C12N15/113
Inventor 李学宝黄耿青许文亮龚思颖李鹏
Owner HUAZHONG NORMAL UNIV
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