The invention discloses a thermostatic strand displacement amplification technology. According to the technology, after heating denaturation is conducted on a target
DNA sequence, a pair of primers containing
RNA sequences are used for hybridizing with a template
DNA single chain, the primers are extended under the action of strand displacement
DNA polymerase, a combined sequence of target DNA andRNA containing an identification site of endoribonuclease is generated, the identification site is
cut by the hydrolyzation of thermal resistant endoribonuclease to form viscous termini, the primersare connected with the viscous termini, a 3' terminus is extended under the action of the strand displacement
DNA polymerase and replaces another DNA strand, a displaced strand is sequentially turnedinto target sources of another amplification reaction after the displaced strand is hybridized with the primers, the steps are constantly repeated in a
reaction system, and the target DNA sequence isincreased in exponential rate. The technology is convenience, sensitive, rapid and good in specificity, the detecting
lower limit is DNA with a plurality of
gene copy numbers, the technical detect ofthermostatic amplification in the prior art is overcome, and the technology can be widely applied to the fields of clinical
medicine, medical jurisprudence, animal and
plant quarantine inspection andthe like.