367 results about "Linear amplification" patented technology

A novel limited primer extension reaction improves detection sensitivity and specificity in a variety of hybridization platforms. In the invention, a sequence of target DNA that lacks one of the four types of nucleic acid bases for a span of eight or more adjacent nucleotide positions is selected for use. This sequence is referred to as the extension complement sequence, or ECS. A primer with a sequence that is complementary to the target sequence that is immediately downstream (to the 3′ side) of this ECS is used to initiate an extension reaction. Extension occurs using a DNA polymerase and standard deoxynucleoside triphosphates for three of the four types of nucleic acid bases. The fourth base, which is complementary to the base missing in the ECS, is either absent or present only in the form of a dideoxynucleoside triphosphate, which does not support further extension. In either case, the extension reaction does not proceed past the first occurrence in the template of the base that is missing in the ECS. This results in a primer extension product with fixed length determined by the length of the ECS. The process can be repeated using a thermal-stable polymerase in a thermal-cycled reaction that results in a linear amplification of the targeted sequence. The resulting limited primer extension products serve as ideal hybridization analytes for determination of sample sequence content using microarrays.

Described herein are novel polynucleotides associated with prostate cancer. The polynucleotides are miRNAs, miRNA precursors, and associated nucleic acids. Methods and compositions are described that can be used for diagnosis, prognosis, and treatment of prostate cancer. Also described herein are methods that can be used to identify modulators of the disease-associated polynucleotides. Also described herein are methods and compositions for linear amplification and labeling of a targeted nucleic acid. The amplified targeted molecules may be used in hybridization techniques like Luminex and Microarray analysis.

A power combining and outphasing system and related techniques for simultaneously providing both wide-bandwidth linear amplification and high average efficiency is described. Providing linear amplification encompasses the ability to dynamically control an RF output power level over a wide range while still operating over a wide frequency bandwidth. The system and techniques described herein also operate to maintain high efficiency across a wide range of output power levels, such that a high average efficiency can be achieved for highly modulated output waveforms.

The invention relates to a method of detecting a target nucleic acid by linear amplification. The invention also relates to a method of detecting a target nucleic acid by amplification.

Methods for isothermal exponential amplification of a target polynucleotide are disclosed. The methods employ two transcription modules, the first module providing linear amplification resulting in RNA transcripts, and a second module providing for further (generally cyclical) amplification resulting in more RNA transcripts. In one aspect, the amplification of the first module is composite primer based. In a second aspect, the amplification of the first module is based on target switching to generate a primer extension product comprising a promoter sequence. In all aspects, the RNA transcripts of the first transcription module are subjected to further amplification by creating an intermediate product comprising a double stranded promoter region from which transcription can occur. The invention further provides compositions and kits for practicing said methods, as well as methods which use the amplification results.

The present invention relates to a high linear enhancement-mode heterostructure field-effect transistor. More, the present invention uses an InGaAs channel structure with a linear change, and integrates an adjusting effect of working region corresponding to the threshold voltage of the element. It not only directly provides a complementary structure for the conventional depletion-mode element to select, but also increases the range of the gate voltage swing. More, some important characteristics, such as current driving capacity, transconductance gain, linear amplification, and high speed operation can be largely improved. More particularly, E-mode working element has a low static power. Further, the present invention also has a high stop frequency characteristic of the high speed element from the composite semiconductor, and it can be applied to the microwave push-pull amplification circuit.

A motor control apparatus of the present invention comprises a velocity detector for detecting a rotation velocity of a mover of a motor, a position detector for detecting a position of the mover, a differentiator for performing time differentiation with respect to the position detected by the position detector to obtain a velocity calculation value, a feedback signal generating circuit for generating a feedback signal including the velocity detection value obtained by the velocity detector as a high frequency component and the velocity calculation value obtained by the differentiator as a low frequency component, and a linear amplifier and an integrating amplifier for performing PI control, the linear amplifier and the integrating amplifier performing linear amplification and integrating amplification of a difference between the velocity instruction and the feedback signal, respectively.