367 results about "Linear amplification" patented technology

The present invention provides for amplification of one or more polynucleotides by multi-staged linear amplifications using one or more RNA polymerases. At each stage RNA transcripts are accumulated at a linear rate, so that multiple stages provide for faster than linear transcript accumulation. In one aspect, the invention provides for polynucleotide amplification by ligating hairpin adaptors to an end of polynucleotides wherein the hairpin adaptors each contain a promoter sequence oriented so that transcription proceeds in the direction of the loop of the hairpin adaptor. Upon transcription through such loop region and to the complementary strand a replicate is made of the promoter sequence as well as the polynucleotide, thereby permitting exponential amplification upon reverse transcription, second strand synthesis, and repetition of the above cycle. Preferably such amplification is carried out under isothermal reaction conditions.

A digital predistortion amplifier design compensates for non-linear amplification of an input signal using predistortion techniques. The design provides a reduced noise floor by using separate digital to analog converters (DAC) to separately convert the input signal and an error correction signal. Furthermore, the input signal can be separated into two or more subbands of narrower bandwidth. Each of the subbands are converted to analog using a separate DAC. By reducing the power and / or bandwidth to be handled by any one DAC, the available levels of quantization of the DAC are applied to a lower power signal and therefore the signal to noise ratio resulting from the conversion process is improved. In addition, each digital subband is passed through a correction filter, which is driven by an adaptive control processing and compensation estimator to compensate for relative gain, phase, and delay inconsistencies between the different subbands.

The invention relates to a method of detecting a target nucleic acid by linear amplification. The invention also relates to a method of detecting a target nucleic acid by amplification. The method of the invention includes the steps of forming at least a first cleavage structure comprising at least one flap, cleaving the cleavage structure with a cleavage means to release the at least one flap and detecting the at least one flap. In one embodiment of the invention, the method includes the additional steps of forming at least a second cleavage structure comprising the at least one released flap from the first cleavage structure cleaving the second cleavage structure with a cleavage means to release at least one flap from the second cleavage structure and detecting released flaps from the first or second cleavage structure.

A novel limited primer extension reaction improves detection sensitivity and specificity in a variety of hybridization platforms. In the invention, a sequence of target DNA that lacks one of the four types of nucleic acid bases for a span of eight or more adjacent nucleotide positions is selected for use. This sequence is referred to as the extension complement sequence, or ECS. A primer with a sequence that is complementary to the target sequence that is immediately downstream (to the 3′ side) of this ECS is used to initiate an extension reaction. Extension occurs using a DNA polymerase and standard deoxynucleoside triphosphates for three of the four types of nucleic acid bases. The fourth base, which is complementary to the base missing in the ECS, is either absent or present only in the form of a dideoxynucleoside triphosphate, which does not support further extension. In either case, the extension reaction does not proceed past the first occurrence in the template of the base that is missing in the ECS. This results in a primer extension product with fixed length determined by the length of the ECS. The process can be repeated using a thermal-stable polymerase in a thermal-cycled reaction that results in a linear amplification of the targeted sequence. The resulting limited primer extension products serve as ideal hybridization analytes for determination of sample sequence content using microarrays.

Described herein are novel polynucleotides associated with prostate cancer. The polynucleotides are miRNAs, miRNA precursors, and associated nucleic acids. Methods and compositions are described that can be used for diagnosis, prognosis, and treatment of prostate cancer. Also described herein are methods that can be used to identify modulators of the disease-associated polynucleotides. Also described herein are methods and compositions for linear amplification and labeling of a targeted nucleic acid. The amplified targeted molecules may be used in hybridization techniques like Luminex and Microarray analysis.

A digital predistortion amplifier design compensates for non-linear amplification of an input signal using predistortion techniques. The design provides a reduced noise floor by using separate digital to analog converters (DAC) to separately convert the input signal and an error correction signal. Furthermore, the input signal can be separated into two or more subbands of narrower bandwidth. Each of the subbands are converted to analog using a separate DAC. By reducing the power and / or bandwidth to be handled by any one DAC, the available levels of quantization of the DAC are applied to a lower power signal and therefore the signal to noise ratio resulting from the conversion process is improved. In addition, each digital subband is passed through a correction filter, which is driven by an adaptive control processing and compensation estimator to compensate for relative gain, phase, and delay inconsistencies between the different subbands.

Described herein are novel polynucleotides associated with prostate cancer. The polynucleotides are miRNAs, miRNA precursors, and associated nucleic acids. Methods and compositions are described that can be used for diagnosis, prognosis, and treatment of prostate cancer. Also described herein are methods that can be used to identify modulators of the disease-associated polynucleotides. Also described herein are methods and compositions for linear amplification and labeling of a targeted nucleic acid. The amplified targeted molecules may be used in hybridization techniques like Luminex and Microarray analysis.

Compositions and methods for amplifying selected polynucleotides, including DNA and RNA, particularly in multiplex amplification reactions using common primers amplification. Generally, methods of the invention employ multiple steps such as template-specific hybridization, a linear amplification, partial degradation of nucleic acid, and ligation. At the end of the process the sequences of selected polynucleotides are flanked by the common sequences which can be used for exponential amplification using common primers. In some aspects the polynucleotides are associated with a barcode and the presence of the barcode is detected to measure the amount of the polynucleotide.

Described herein are novel polynucleotides associated with liver cancer. The polynucleotides are miRNAs, miRNA precursors, and associated nucleic acids. Methods and compositions are described that can be used for diagnosis, prognosis, and treatment of liver cancer. Also described herein are methods that can be used to identify modulators of the disease-associated polynucleotides. Also described herein are methods and compositions for linear amplification and labeling of a targeted nucleic acid. The amplified targeted molecules may be used in hybridization techniques like Luminex and Microarray analysis.

A power combining and outphasing system and related techniques for simultaneously providing both wide-bandwidth linear amplification and high average efficiency is described. Providing linear amplification encompasses the ability to dynamically control an RF output power level over a wide range while still operating over a wide frequency bandwidth. The system and techniques described herein also operate to maintain high efficiency across a wide range of output power levels, such that a high average efficiency can be achieved for highly modulated output waveforms.

To mitigate the effects of non-linear amplification in a digital transmission system employing a multi-carrier multiplexing communications technique, such as orthogonal frequency division multiplexing or discrete multi-tone, predistortion of an input signal to a power amplifier with a non-linear transfer characteristic is performed in the frequency domain by modifying frequency-domain symbols used to modulate individual sub-carriers. The frequency-domain symbols are modified in a manner that compensates for the amplifier's non-linear transfer characteristic.

Compositions and methods for nucleic acid amplification are provided that minimize the formation and amplification of spurious products, particularly in multiplex amplification reactions. Linear amplification molecules are provided. A first embodiment comprises a first common primer sequence, a first template homology region, a cleavable site, a second common primer sequence and a second template homology region. Methods employing the linear amplification molecules for nucleic acid amplification are also provided. A first embodiment comprises a template-specific hybridization, a linear amplification, a template-specific intramolecular hybridization, a strand cleavage, a second linear amplification and an exponential amplification using common primers.

The present invention relates to an in-vessel or down-hole optical imaging sensor or system for operating in structures which may contain media with different spectral transmission characteristics. The imaging sensor of the present invention selectively emits and / or detects two or more independently controllable wavelengths or wavebands. The imaging sensor comprises an illuminator for emitting radiation of a specified wavelength or waveband through a medium to a target, at least one detector for detecting the radiation deflected by said target and at least one amplifier for providing non-linear amplification of the detector output.

A protection device for gas water heater flue blockage comprises a fan and a controller, and further comprises a rotation speed detecting circuit, a current detecting circuit, a voltage detecting circuit, and a linear amplification and filter circuit, wherein the fan is an alternating current fan, the voltage detecting circuit is respectively connected with the fan and the controller electrically, one end of the current detecting circuit is electrically connected with the controller through the linear amplification and filter circuit, the other end of the current detecting circuit is electrically connected with the fan, one end of the rotation speed detecting circuit is connected with the fan, and the other end of the rotation speed detecting circuit is electrically connected with the controller. A detecting method of the protection device comprises the steps of setting working parameters of the fan in all burning working stations and all blockage degrees and the like. Under the condition that an air pressure switch is not needed, the protection device can control the fan to work according to different blockage degrees of a flue, and when the flue is blocked to a certain degree, the water heater is switched off timely and accurately so that use safety of the water heater is guaranteed.

The present invention provides primers for use in cycle sequencing which are not subject to exponential amplification of undesired artifacts. Such primers cannot be replicated by the nucleic acid polymerases used in these reactions and, therefore, do not produce artifacts. Methods of linear amplification of a nucleic acid template using such primers are also provided.

The invention relates to a method of detecting a target nucleic acid by linear amplification. The invention also relates to a method of detecting a target nucleic acid by amplification.

The invention relates to a pulse electronic accelerator nano-pico ampere electron beam flow measuring system, relating to an electronic accelerator pulse electron beam flow measuring system, in particular to the nano-pico (10-10A / cm2) micro beam flow measurement of millisecond pulse, belonging to the electron radiation environment ground simulation and test field. The pulse electronic accelerator nano-pico ampere electron beam flow measuring system is composed of a novel inclined-bottom faraday cup, three coaxial cables and a nano-pico level current amplifier. The measurement result can be obtained by oscilloscope or data acquisition card. The inclined-bottom faraday cup electron collection system resolves the collection efficiency problem of high energy electrons (MeV), to improve collection efficiency. The adopted three coaxial cables can reduce the interference of electromagnetic field. The nano-pico ampere level current amplifier resolves the stable current linear amplification problem under electron radiation, and converts weak current signals to voltage signals, thus being convenient for data acquisition of oscilloscope or data acquisition card. The invention resolves the measurement problem of nano-pico ampere pulse electron beam flow, to confirm the accuracy, real-time property and easy use of measurement.

A system and method is provided to identify UDP attacks. A processor determines a spectral density of packet timing intervals, a natural distance between the spectral density and a uniform distribution, and a non-linear amplifier applying a non-linear amplification to the natural distance to detect a denial-of-service attack. It uses the concept of traffic statistics analysis, i.e., spectral densities of arrived-packet timing intervals, calculates the KL-distance measurement and makes decision based on the output of a non-linear Gaussian amplifier, with which one can easily adjust the amplifier via selecting different parameters of mean and variance to satisfy system requirements of false-positive and false-negative UDP attack detections.

An RF power amplifier has a final-stage amplifier stage which generates an RF transmit output signal, a signal detector which detects an RF transmit output level, a first detector, a second detector and a control circuit. The final-stage amplifier stage includes a transistor and a load element and performs saturation type nonlinear amplification and non-saturation type linear amplification. The first detector and the control circuit maintain the RF transmit output signal approximately constant with respect to a variation in load at an antenna at the saturation type nonlinear amplification. The second detector and the control circuit reduce an increase in the output voltage of the final stage transistor with respect to an overload state of the antenna at the non-saturation type linear amplification.

A power combining and outphasing system and related techniques for simultaneously providing both wide-bandwidth linear amplification and high average efficiency is described. Providing linear amplification encompasses the ability to dynamically control an RF output power level over a wide range while still operating over a wide frequency bandwidth. The system and techniques described herein also operate to maintain high efficiency across a wide range of output power levels, such that a high average efficiency can be achieved for highly modulated output waveforms.

A motor control apparatus of the present invention comprises a velocity detector for detecting a rotation velocity of a mover of a motor, a position detector for detecting a position of the mover, a differentiator for performing time differentiation with respect to the position detected by the position detector to obtain a velocity calculation value, a feedback signal generating circuit for generating a feedback signal including the velocity detection value obtained by the velocity detector as a high frequency component and the velocity calculation value obtained by the differentiator as a low frequency component, and a linear amplifier and an integrating amplifier for performing PI control, the linear amplifier and the integrating amplifier performing linear amplification and integrating amplification of a difference between the velocity instruction and the feedback signal, respectively.

Methods for isothermal exponential amplification of a target polynucleotide are disclosed. The methods employ two transcription modules, the first module providing linear amplification resulting in RNA transcripts, and a second module providing for further (generally cyclical) amplification resulting in more RNA transcripts. In one aspect, the amplification of the first module is composite primer based. In a second aspect, the amplification of the first module is based on target switching to generate a primer extension product comprising a promoter sequence. In all aspects, the RNA transcripts of the first transcription module are subjected to further amplification by creating an intermediate product comprising a double stranded promoter region from which transcription can occur. The invention further provides compositions and kits for practicing said methods, as well as methods which use the amplification results.

The invention relates to a method and an instrument for measuring the shock ground resistance of a transmission line tower. The method comprises the following steps: generating incident current by a shock current generator and filling the incident current into the ground; collecting the grounding voltage and the current of the tower by a data collecting device; carrying out treatment and linear amplification on the collected data by an MCU control panel to solve the voltage response of a grounding body under the action of standard lightning current and obtain an equivalent voltage um and a current peak im; and finally, obtaining the shock ground resistance of the tower according to a formula of Rch=um / im. The instrument comprises a current pole lead connecting plug, a grounding device connecting plug, controlled silicon, a charging board, a voltage-multiplying generator, a voltage collecting board, a current collecting board, a battery and a main control board, wherein the current polelead connecting plug is connected with the charging board and the voltage-multiplying generator and then connected to the main control board; the controlled silicon is connected to a circuit in series; the voltage collecting board is connected with a voltage pole lead connecting plug, the grounding device connecting plug and the main control board; the current collecting board is connected with the current pole lead connecting plug, the grounding device connecting plug and the main control device; and the battery is directly connected to the main control board.

Methods for isothermal exponential amplification of a target polynucleotide are disclosed. The methods employ two transcription modules, the first module providing linear amplification resulting in RNA transcripts, and a second module providing for further (generally cyclical) amplification resulting in more RNA transcripts. In one aspect, the amplification of the first module is composite primer based. In a second aspect, the amplification of the first module is based on target switching to generate a primer extension product comprising a promoter sequence. In all aspects, the RNA transcripts of the first transcription module are subjected to further amplification by creating an intermediate product comprising a double stranded promoter region from which transcription can occur. The invention further provides compositions and kits for practicing said methods, as well as methods which use the amplification results.

The invention provides a combined pre-coding and power allocation method of the multi-cast network based on the network coding in the wireless communication field. In the invention, a multi-cast network formed by two-source, two-place and arbitrary relaying cluster is adopted. Two sinks need to obtain the information of each source and can not cross-transmit the signal due to the limit of the sending power, so the relaying node is responsible for forwarding information of each source. Through introducing simulation network coding into the relaying, namely, the received aliasing signal of the two sources is broadcast out after linear amplification, thus minimizing the transmission time to the maximum extent. The invention improves the network error frame performance and enhances the overall through put of the network by introducing the combined pre-coding and power allocation.

A transmitter capable of carrying out a highly efficient polar coordinate modulation while maintaining an excellent distortion characteristic. When a high power amplifier is operated in a saturation mode to carry out a polar coordinate modulation, a switching control signal is set to be a multiplication, and a quadrature modulating digital signal and distortion data of a compensation table are multiplied, thereby adding a nonlinear distortion equivalent to that of the amplifier in a linear mode and compressing a peak factor of a modulating signal to enhance an efficiency of the polar coordinate modulation. When the amplifier is operated in the linear mode to carry out a linear amplification, the switching control signal is set to be a division and the quadrature modulating digital signal and the distortion data are divided, thereby adding a reverse distortion characteristic of the amplifier to carry out a nonlinear distortion compensation of the amplifier.