Primers, micro-fluidic chip and system for swine fever virus detection, and application of primers
A microfluidic chip, swine fever virus technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. To solve the problem of high needle cost, to achieve the effect of improved detection convenience, improved analytical sensitivity and high accuracy
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[0049] Find the target sequence through NCBI GenBank, and design primers for the target sequence. The designed primer sequences are shown in Table 1 below (the corresponding sequence list is provided with sequence order):
[0050] Table 1 Primer Sequence
[0051] P1 AGGGACTAGCCGTAGTGG P2 AGGTCGTACCCCCATCAC P3 GGTGAGCTTCTGCTCATGTCGA-AGCTCCCTGGGTGGTCTA P4 CGAGATGCTATGTGGACGAGGG-TGTGAGTTCACCCTAGCGA
[0052] And fix them on the corresponding positions of the microfluidic chip, then package the microfluidic chip, mix the reaction solution, reverse transcriptase, inhibitor with pork and its various organs, blood, excrement, environmental samples and After the nucleic acid template extracted from the cell culture is mixed and reacted, it is added to the packaged microfluidic chip, and then put into the microfluidic chip detector with centrifugal function, constant temperature function and real-time fluorescence detection, driven by centrifugal force ...
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