32 results about "Ubiquitin-conjugating enzyme" patented technology

A method for producing a plant with increased yield as compared to a corresponding wild type plant whereby the method comprises at least the following step: increasing or generating in a plant or a part thereof one or more activities of a polypeptide selected from the group consisting of 2-oxoglutarate-dependent dioxygenase, 3-ketoacyl-CoA thiolase, 3′-phosphoadenosine 5′-phosphate phosphatase, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, 5OS chloroplast ribosomal protein L21, 57972199. R01.1-protein, 60952769. R01.1-protein, 60S ribosomal protein, ABC transporter family protein, AP2 domain-containing transcription factor, argonaute protein, AT1 G29250.1-protein, AT1 G53885-protein, AT2G35300-protein, AT3G04620-protein, AT4G01870-protein, AT5G42380-protein, AT5G47440-protein, CDS5394-protein, CDS5401_TRUNCATED-protein, cold response protein, cullin, Cytochrome P450, delta-8 sphingolipid desaturase, galactinol synthase, glutathione-S-transferase, GTPase, haspin-related protein, heat shock protein, heat shock transcription factor, histone H2B, jasmonate-zim-domain protein, mitochondrial asparaginyl-tRNA synthetase, Oligosaccharyltransferase, OS02G44730-protein, Oxygen-evolving enhancer protein, peptidyl-prolyl cis-trans isomerase, peptidyl-prolyl cis-trans isomerase family protein, plastid lipid-associated protein, Polypyrimidine tract binding protein, PRLI-interacting factor, protein kinase, protein kinase family protein, rubisco subunit binding-protein beta subunit, serine acetyltransferase, serine hydroxymethyltransferase, small heat shock protein, S-ribosylhomocysteinase, sugar transporter, Thioredoxin H-type, ubiquitin-conjugating enzyme, ubiquitin-protein ligase, universal stress protein family protein, and Vacuolar protein.

Ubiquitin ligase wwp-1 and ubiquitin conjugating enzyme ubc-18 are identified in nematodes as mediators of dietary restriction induced longevity and therefore as targets for modulation of lifespan in animals. Methods of screening for compounds that modulate longevity by assaying wwp-1 ubiquitination pathway parameters are provided, as are related systems. In addition, methods of using wwp-1 and / or ubc-18 to modulate longevity or delay onset of age-related diseases are described.

The present invention relates to novel uses for ubiquitin conjugating enzyme E2-EPF5. In particular, inhibition of E2-EPF5 activity is shown to reduce the production VEGF, as well as other proteins regulated the transcription factor HIF-1, in response to hypoxia. Based on these findings the present invention provides therapeutic methods and pharmaceutical compositions useful for the treatment of diseases related VEGF induced angiogenesis. In addition, E2-EPF5 is provided as target for the development of therapeutics. Accordingly, the invention provides screening methods for identifying candidate compounds that inhibit E2-EPF5 activity and may therefore be used to treat VEGF induced angiogenesis such as tumor related angiogenesis. Finally, the invention also provides methods for the inhibition of VEGF and other HIF-1 regulated proteins.

An isolated nucleic acid molecule encoding human skeletal muscle-specific ubiquitin-conjugating enzyme and comprising a nucleotide sequence coding for the amino acid sequence shown in SEQ ID NO:22 is disclosed. The isolation of this molecule makes it possible to detect its expression in various tissues, analyze its structure and function, and produce the human proteins encoded by this molecule by the technology of genetic engineering. In this way, it is possible to analyze the corresponding expression products, elucidate the pathology of diseases associated with the molecule, for example hereditary diseases and cancer, and diagnose and treat such diseases.

A polynucleotide isolated from soybean plants capable of initiating transcription and with sequence identity to SEQ ID No. 1 is provided. In some aspects, the polynucleotide has sequence identity to SEQ ID No. 1 of at least 40%, is the reverse complement or the reverse of such sequences. In some aspects, the polynucleotide is linked to expression enhancers or sequences of interest. In some embodiments, a recombinant vector comprises the polynucleotide. In some aspects, the recombinant vector comprises enhancers, termination sequences, or sequences of interest. In some embodiments a transformed cell, plant, plant part, or propagulum comprise the polynucleotide.

The present Invention relates to regulatory sequences of polynucleotides isolated from cotton plants capable of initiating and activating the transcription of polynucleotides, and the use of these regulatory sequences for modifying the transcription of endogenous and / or heterologous polynculeotides and the production of polypeptides.

The invention discloses a human ubiquitin-conjugating enzyme UbcH10 monoclonal antibody hybrid tumor DY03 and a monoclonal antibody. The human ubiquitin-conjugating enzyme UbcH10 monoclonal antibody hybrid tumor DY03 is collected in the China General Microbiological Culture Collection Center (CGMCC) with the collection number of CGMCC No.4336 on November 19, 2010. The produced monoclonal antibody can be specifically recombined with a natural human ubiquitin-conjugating enzyme UbcH10 protein and can be applied to ELISA (Enzyme-Linked Immuno Sorbent Assay), western-blot, cell immunofluorescence and immune tissue chemical staining research of clinical tissue samples. Meanwhile, the antibody can be prepared into an immunodetection reagent for laboratory and clinical detection of the expression situation of the human ubiquitin-conjugating enzyme UbcH10.

The invention discloses a system and a method for in vitro detection of activity of a Ub (ubiquitin)-conjugating enzyme. The system comprises a prokaryotic expression strain C1 competent cell capable of expressing E1 protein, a prokaryotic expression bacterium C2 competent cell capable of expressing the E1 protein and Ub protein simultaneously and a plasmid P3 having different replication origins from those of plasmids expressing the E1 protein and Ub protein and being capable of expressing to-be-detected E2 protein in vitro of protokaryon. P3 is transferred into the C1 competent cell and the C2 competent cell, named as C3CK (negative control) and C3 respectively, amplified and cultured respectively, the bacteria are collected after induction of protein expression, and protein western blot is performed; a migration stripe of the to-be-detected E2 protein is detected in a C3 sample, and the migration size conforms to the Ub molecular weight, therefore, the condition that the to-be-detected E2 protein has activity of the Ub-conjugating enzyme is proved. According to the method, neither lysis of cells for protein extraction and purification nor in vitro response is required, the method is quite convenient, and results are more stable.

Provided are methods of treating or preventing Ca2 + / calmodulin-dependent kinase II (CaMKII) mediated diseases, methods of reducing heart damage, methods of stimulating the activity of an ubiquitin-binding enzyme, methods of preventing degradation of an ubiquitin-binding enzyme, methods of preventing cardiac muscle cell death, methods of reducing DNA damage in a cell, methods for diagnosing CaMKII mediated diseases, and methods of treating or preventing CaMKII mediated diseases. The invention relates to CaMKII (CaMKII)-mediated diseases, a kit for diagnosing the CaMKII-mediated diseases, a biomarker for diagnosing the CaMKII-mediated diseases, and application of CaMKII delta 9 as the biomarker for diagnosing the CaMKII-mediated diseases. Also provided herein are methods for identifying molecules, isolated polypeptides, isolated nucleic acids, and antagonists thereof.

The invention provides application of diaphorina citri ubiquitin-binding enzyme E2J2 gene in prevention and control of citrus huanglongbing, and belongs to the technical field of animal gene engineering. The invention provides the application of the diaphorina citri ubiquitin-binding enzyme E2J2 gene with a nucleotide sequence as shown in SEQ ID NO.1 in prevention and control of the citrus huanglongbing. A specific primer is designed to synthesize dsRNA, the silent E2J2 gene is interfered by RNA, the gene silencing efficiency of the gene is detected, the gene is subsequently placed on a branch infected with Candidatus Liberibacter asiaticum for feeding, and the influence of the gene on Candidatus Liberibacter asiaticum (CLas) infection is studied. By blocking the transmission path of the liberobacter asiaticum in the diaphorina citri instead of controlling the diaphorina citri, a new strategy is provided for effective prevention and control of the liberobacter asiaticum, and a new prevention and control thought is provided for controlling transmission of bacterial pathogens by means of vector insects.

Ubiquitin ligase wwp-1 and ubiquitin conjugating enzyme ubc-18 are identified in nematodes as mediators of dietary restriction induced longevity and therefore as targets for modulation of lifespan in animals. Methods of screening for compounds that modulate longevity by assaying wwp-1 ubiquitination pathway parameters are provided, as are related systems. In addition, methods of using wwp-1 and / or ubc-18 to modulate longevity or delay onset of age-related diseases are described.

The invention discloses a wild grape ubiquitin conjugating enzyme gene sequence, which firstly obtains a key gene-ubiquitin conjugating enzyme gene of a protein ubiquitination path by adopting the technology of constructing a cDNA library for carry out the cloning of a wild grape powdery mildew resistance gene; the encoded amino acid sequence of the gene has an HOTLOOPS structural sequence in 1 to 30 amino acid residues, a conservative catalytic domain UBCc structural domain which is shared by all the ubiquitin conjugating proteins exists between the 32 and 174 amino acid residues; the total length of the protein amino acid sequence is 183 amino acids, molecular weight is 20.8KD, isoelectric point is 8.23, and the encoded protein of the gene pertains to an ubiquitin conjugating protease E2. The construction of the sequence provides a molecular basis for the further study of the wild grape ubiquitin conjugating enzyme gene expression mechanism.

The invention discloses for the first time that the ubiquitin-conjugating enzyme E2 inhibitor can be used as an anti-tumor synergist / drug resistance reversal agent of oncolytic virus. The inventors can significantly enhance the oncolytic effect of oncolytic viruses by inhibiting ubiquitin-conjugating enzyme E2. The inventors used Bay11-7082, a compound that inhibits the activity of ubiquitin-conjugating enzyme E2, to act on tumor cells in cooperation with oncolytic viruses, especially M1 viruses. The experimental results found that Bay11-7082 can cooperate with oncolytic viruses to enhance the anti-tumor effect.

The invention discloses a human ubiquitin-conjugating enzyme UbcH10 monoclonal antibody hybrid tumor DY02 and a monoclonal antibody. The human ubiquitin-conjugating enzyme UbcH10 monoclonal antibody hybrid tumor DY02 is collected in the China General Microbiological Culture Collection Center (CGMCC) with the collection number of CGMCC No.4335 on November 19, 2010. The produced monoclonal antibody can be specifically recombined with a natural human ubiquitin-conjugating enzyme UbcH10 protein and can be applied to ELISA (Enzyme-Linked Immuno Sorbent Assay), western-blot, cell immunofluorescence and immune tissue chemical staining research of clinical tissue samples. Meanwhile, the antibody can be prepared into an immunodetection reagent for laboratory and clinical detection of the expression situation of the human ubiquitin-conjugating enzyme UbcH10.

The present invention provides methods for identifying compounds that selectively bind one or more active sites within an ubiquitin conjugating enzyme. The compounds identified by the methods are useful in the treatment of disorders attributed to dysregulated ubiquitin conjugating enzyme function, specifically in hyperproliferative disorders.

The invention discloses a fenneropenaeus chinensis ubiquitin-conjugating enzyme gene with a nucleotide sequence expressed as SEQ ID No. 1, and provides a recombinant expression and purification method thereof. The invention also provides ubiquitin-conjugating enzyme coded by the fenneropenaeus chinensis ubiquitin-conjugating enzyme gene, wherein the amino acid sequence of the ubiquitin-conjugating enzyme is expressed as SEQ ID No. 2; and the invention provides application of the gene sequence and application of the ubiquitin-conjugating enzyme, namely the fenneropenaeus chinensis ubiquitin-conjugating enzyme gene can be used for preparing a prokaryotic expression vector, transforming escherichia coli and expressing recombinant protein with antiviral activity. The recombinant ubiquitin-conjugating enzyme protein obtained by using the invention can be used for antiviral feed additive, animal and plant gene transformation and medicament development.