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36 results about "Dye binding" patented technology

Dye binding, being a dissociation-association equilibrium phenomenon, is influenced by changes in temperature; a rise in temperature increases the dye-albumin dissociation and thus gives lower results. Therefore, standards and sera should be run under identical conditions.

Nir materials and nanomaterials for theranostic applications

Novel fluorescent dye comprising metal oxide nanoparticles are prepared where the nanoparticles are as small as 3 nm or up to 7000 nm in diameter and where the dye is bound within the metal oxide matrix. In some embodiments the invention, novel dyes are covalently attached to the matrix and in other embodiments of the invention a dye is coordinate or ionic bound within the metal oxide matrix. A method for preparing the novel covalently bondable modified fluorescent dyes is presented. A method to prepare silica comprising nanoparticles that are 3 to 8 nm in diameter is presented. In some embodiments, the fluorescent dye comprising metal oxide nanoparticles are further decorated with functionality for use as multimodal in vitro or in vivo imaging agents. In other embodiments of the invention, the fluorescent dye comprising metal oxide nanoparticles provide therapeutic activity and incorporated therapeutic temperature monitoring.
Owner:UNIV OF FLORIDA RES FOUNDATION INC

Genetic engineering of cotton to increase fiber strength, water absorption and dye binding

An expression cassette having a fiber specific promoter driving expression of a gene encoding an elastic and plastic protein based polymer having the repetitive amino acid sequence Gly-Val-Gly-Val-Pro (SEQ. ID. NO. 2), a terminator, and selectable marker genes for transforming plant cells and a transgenic cotton plant having fiber cells stably transformed with the gene encoding the protein based polymer wherein the cotton fiber cells exhibit improved water absorption, temperature transition properties, fiber strength, elasticity, and dye binding capacity.
Owner:AUBURN UNIV

Metamaterial Optical Elements Self-Assembled on Protein Scaffolds

Protein scaffolds from tobacco mosaic virus coat protein modified to incorporate polyhistidine can bind to a metal or a dye while having improved self-assembly characteristics. The scaffold can take the form of tubes or disks, and can further be formed into dual plasmonic ring resonators. Such self-assembled structures provide useful optical properties.
Owner:UNITED STATES OF AMERICA

Method for determining microprotein in near infrared wavelength zone based on dye binding principle

A method for detecting microprotein in near-infrared region based on dye binding principle includes adding the aqueous solution of protein in bottle with incremental concentrations, adding acidic buffer solution, diluting with water, adding cyanine solution, standing and scanning absorption spectrum or measuring the increment of light absorption degree. Its advantages are high sensitivity and less interference.
Owner:XIAMEN UNIV

Hydroxamate substituted azaindoline-cyanine dyes and bioconjugates of the same

Hydroxamate substituted azaindoline cyanine dyes, conjugates thereof and methods of using the same are provided. The subject cyanine dyes include an azaindoline ring having a hydroxamate substituent. The dyes may further include a reactive group moieties (RGM) and / or a water soluble group. Also provided are conjugates of the subject dyes. Also provided are tandem conjugates including a fluorescent protein capable of energy transfer to the dye. Methods of detecting an analyte in a sample by contacting the sample with a detection reagent are provided. The detection agent may be a dye-conjugate that specifically binds the analyte, or may be a reactive dye which conjugates to the analyte. Also provided are compositions, e.g., kits, etc., incorporating such dyes which facilitate use in such methods.
Owner:BECTON DICKINSON & CO

Composite particle, contrast agent for photoacoustic imaging, and photoacoustic imaging method

Regarding a composite particle in the related art, an antibody and a dye are bound to the surface of the particle. Therefore, it is not possible to bind both the antibody and the dye high in number. Accordingly, the present invention provides a composite particle, wherein a large acoustic wave can be emitted and both the antibody and the dye can be bound high in number. A composite particle including a core particle, a dye bound to the above described core particle, and an antibody, wherein the above described antibody is bound to the above described dye, is suitable for use.
Owner:CANON KK

Method for measuring lysine in corn through near infrared spectrum

The invention relates to a method for measuring lysine in corn through a near infrared spectrum. The method comprises the following steps of: testing a spectrum, rejecting an abnormal value, establishing an analysis model and the like. By the method, the lysine content can be rapidly and nondestructively measured without pretreatment, and the method is particularly suitable for measuring the lysine content of low-generation materials in the process of breeding high-quality protein corn; compared with lysine content measured results in a dye binding method (DBL), a ninhydrin development process and an amino acid analyzer method, the lysine content measured result in the method verifies the feasibility of a method for measuring the corn through the near infrared spectrum, particularly lysine of the low-generation materials in the process of breeding the high-quality protein corn; moreover, the method is rapid, reliable and practical, and has certain reference value for selecting a method for measuring the lysine content in the process of breeding the high-quality protein corn.
Owner:HENAN ACAD OF AGRI SCI

Preparation method of functional impact-resistant veneer

The invention discloses a preparation method of a functional impact-resistant veneer; the veneer is processed through dyeing and drying, resin preparation, resin impregnation and drying balance, so that the veneer has good and lasting impact resistance and attractiveness, and the service life of the veneer is prolonged. Specifically, the problems of cracking, warping deformation and the like are avoided by controlling the moisture content of the veneer in the technological process, the strength of the veneer is kept, on the basis, the veneer is coated with modified resin with modified PAE as amain raw material, a vacuum impregnation technology is combined, and thus the impact strength, the size stability and the waterproofness of the veneer are improved; a specific compound dye composed of a hindered amine light stabilizer and a bisazo acid dye is combined with a vertical dip dyeing process, so that the dyeing effect is attractive and lasting, meanwhile, the modified resin isolates the environment, and the color fastness of the veneer is further improved.
Owner:DEHUA TB NEW DECORATION MATERIAL CO LTD

Detergent compositions and copolymers for inhibiting dye transfer

A detergent composition comprising a copolymer and at least one component selected from surface active agents and fabric softening compounds, the copolymer comprising at least one dye binding monomer and at least one anionic or nonionic monomer, with the proviso that the copolymer is not poly(1-vinylpyrrolidone-co-acrylic acid). Also a method of mitigating dye transfer in a detergent composition in the presence of typically encountered soils is provided. A dye binding copolymer comprising at least one anionic monomer selected from acrylic acid, methacrylic acid, vinyl sulphonic acid, itaconic acid, octanoic acid, hexanoic, hydroxy acetic acid or mixtures thereof or at least one nonionic monomer selected from polyethylene glycols and at least one dye binding monomer selected from vinyl imidazole, methyl vinyl imidazole, vinyl pyridine-N-oxide, vinyl pyrrolidone and mixtures thereof is also disclosed.
Owner:UNILEVER NV

Materials and methods for rapid and specific detection of synthetic cathinones

ActiveUS10683507B2Facilitating interference-free detectionMethine/polymethine dyesOrganic chemistryAptamerAssay
The subject invention provides methods, assays, and products for detecting small molecules in a sample, in particular, in both clinical and field settings. The method for detecting a small-molecule target, preferably, a synthetic cathinone in a sample comprises contacting the sample with an aptamer-based sensor selective for the small-molecule target, and detecting the small-molecule target in the sample. Specifically, the method utilizes an aptamer-based sensor comprising a dye binding to a three-way junction binding domain of an aptamer. Binding of small-molecule target to the aptamer displaces the dye, generating a spectroscopic signal that can be used for detection of the small-molecule target and quantitative measurement of the target concentration.
Owner:FLORIDA INTERNATIONAL UNIVERSITY

Materials and methods for rapid and specific detection of synthetic cathinones

ActiveUS20190256852A1Facilitating interference-free detectionMinimal overlapOrganic chemistryMethine/polymethine dyesSpecific detectionBinding domain
The subject invention provides methods, assays, and products for detecting small molecules in a sample, in particular, in both clinical and field settings. The method for detecting a small-molecule target, preferably, a synthetic cathinone in a sample comprises contacting the sample with an aptamer-based sensor selective for the small-molecule target, and detecting the small-molecule target in the sample. Specifically, the method utilizes an aptamer-based sensor comprising a dye binding to a three-way junction binding domain of an aptamer. Binding of small-molecule target to the aptamer displaces the dye, generating a spectroscopic signal that can be used for detection of the small-molecule target and quantitative measurement of the target concentration.
Owner:FLORIDA INTERNATIONAL UNIVERSITY

Metamaterial optical elements self-assembled on protein scaffolds

Protein scaffolds from tobacco mosaic virus coat protein modified to incorporate polyhistidine can bind to a metal or a dye while having improved self-assembly characteristics. The scaffold can take the form of tubes or disks, and can further be formed into dual plasmonic ring resonators. Such self-assembled structures provide useful optical properties.
Owner:THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE NAVY

Asymmetric metal complex black dye and preparation method thereof

The invention relates to an asymmetric metal complex black dye and a preparation method thereof. Specifically, the asymmetric metal complex black dye has a structure represented by the following formula (1). The dye combines the advantages of acid dyes and reactive dyes, and is suitable for printing dyeing of fiber materials containing hydroxyl or amino groups. The dyeing process is simple, stableand reliable, and the dyeing effect is excellent. The material dyed by the dye has good fastness to wet and fastness to daylight.
Owner:上海青石化学有限公司

Binary DNA probe for fluorescent analysis of nucleic acids

The invention is directed to binary oligonucleotide probes for nucleic analysis, which probes can be made of DNA or RNA that recognize nucleic acid analytes (both DNA and RNA) with unprecedented high selectivity under mild conditions and are highly sensitive to single nucleotide mismatches (SNP single nucleotide polymorphisms) without PCR amplification. In one group, the binary probes indicate that they have hybridized to a particular nucleic analyte by binding to a molecular beacon that gives off a fluorescent signal. A second group of binary probes bind to a dye such as malachite green, where upon hybridization to analyte the fluorescence of the dye increases dramatically and is easily detected and measured. The new binary probes require only about five minutes at room temperature to generate a detectable signal.
Owner:THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK

Method for rapidly detecting white spirit based on nanometer material-organic dye colorimetric sensing array

The invention discloses a method for quickly detecting white spirit based on a nano-material organic dye colorimetric sensor array. The method comprises the following steps of introducing six nano-materials and three dyes to construct a nano-material / dye composite colorimetric sensor array, and extracting change values (delta RGB) of array points RGB before and after reaction as detection signals; and processing the data by utilizing pattern recognition and a radial basis function neural network so as to explore the performance of the array detection white spirit. Compared with the prior art, the method has high recognition and detection capacity for different white spirits, combines a nano material with a traditional dye, improves recognition sensitivity of the array, reduces the number of array points, is simple in data processing, has the advantages of simple operation, short test time, high detection efficiency, good sensitivity and the like, and is suitable for popularization and application. The method has a good application prospect in the aspect of on-site real-time detection of white spirit.
Owner:LUZHOU PINCHUANG TECH CO LTD

Near-infrared fluorescent probe compound taking N-pyridine oxide derivative as recognition group as well as preparation and application of near-infrared fluorescent probe compound

The invention discloses a near-infrared fluorescent probe compound taking an N-pyridine oxide derivative as a recognition group as well as preparation and application of the near-infrared fluorescent probe compound. The preparation method specifically comprises the following steps: firstly, oxidizing a pyridine heterocyclic compound into an N-pyridine-2-formaldehyde oxide derivative, and taking the N-pyridine-2-formaldehyde oxide derivative as a recognition group; finally, enabling rhodamine B hydrazide to react with the N-pyridine oxide derivative in an organic solvent, so as to obtain the N-pyridine oxide-rhodamine B near-infrared fluorescent probe. The N-pyridine oxide-rhodamine B type near-infrared fluorescent probe can detect divalent palladium ions with high sensitivity and high selectivity, can realize colorimetric and fluorescent dual-mode detection of the divalent palladium ions in the environment, and can also be used for rapid detection of the divalent palladium ions in cells. Besides, the applied N-pyridine oxide derivative is a new recognition group which is easily available in initial raw material and easy to prepare, and has a wide application prospect in the aspect of detecting other heavy metal ions after being combined with other common dyes.
Owner:GUANGZHOU YUEWANG AGRI CO LTD

A method based on enzymatic modification to improve the dyeing performance of silk fibers

The invention discloses a method for improving dyeing performance of a real silk fabric based on enzymatic modification; serine hydroxymethyltransferase is used for catalyzing glycine in protein fibermacromolecules to be converted into serine, the number of the serine in real silk fiber is increased, phosphorylation of the serine and threonine residues in the real silk fiber is catalyzed by protein kinase A, the cationic dye binding effect is enhanced, and the dyeing performance of the real silk fabric is improved. The method specifically comprises the following steps: carrying out enzymaticconversion of amino acids in real silk; and (2) catalyzing the phosphorylation by the protein kinase A. Compared with the modification and improvement of the electronegativity of the surface of the real silk fiber by applying a chemical method, the method has the advantages of being high in enzyme catalysis efficiency, mild in reaction conditions and obvious in dyeing performance improvement.
Owner:JIANGNAN UNIV

Method for determining microprotein in near infrared wavelength zone based on dye binding principle

A method for detecting microprotein in near-infrared region based on dye binding principle includes adding the aqueous solution of protein in bottle with incremental concentrations, adding acidic buffer solution, diluting with water, adding cyanine solution, standing and scanning absorption spectrum or measuring the increment of light absorption degree. Its advantages are high sensitivity and less interference.
Owner:XIAMEN UNIV

Fluorescent biosensor for 2', 3'-cgamp

A single stranded nucleic acid biosensor for 2′, 3′-cGAMP is provided. The single stranded nucleic acid may include a 2′, 3′-cGAMP-binding riboswitch domain comprising a transducer stem and a dye-binding aptamer domain that is operably connected to the transducer stem. A 2′, 3′-cGAMP-binding riboswitch domain. The dye-binding aptamer domain can be a Spinach2 aptamer. The 2′, 3′-cGAMP biosensor may further include a signaling chromophore specifically bound to the Spinach2 aptamer domain, where the sensor is configured to fluorescently activate the signaling chromophore upon specific binding of 2′, 3′-cGAMP to the 2′, 3′-cGAMP-binding riboswitch domain. Also provided are methods in which the subject 2′, 3′-cGAMP biosensors find use including methods for determining the level of cGAS activity in a sample or a cell. Nucleic acid constructs and host cells including the same are also provided.
Owner:RGT UNIV OF CALIFORNIA

Erythropoietin solution preparation

The present invention relates to an erythropoietin-containing solution preparation containing a poloxamer and having a pH of 6.5 to 7.5. The present invention also relates to a method for quantifying a protein in a trace amount, the method including the following steps: binding a protein sample to a high-intensity fluorescent dye; separating a desired analyte from the obtained sample by an appropriate separation means; and quantifying the desired analyte and converting the amount of the analyte into the amount of the protein.
Owner:CHUGAI PHARMA CO LTD

Quantification of RNA

The present invention relates to a method for real-time monitoring and / or quantification of newly-synthesized complementary deoxyribonucleic acid (cDNA) during a reverse transcription reaction of an ribonucleic acid (RNA) template in a sample, the method using a fluorogenic dye binding to RNA:cDNA hybrids. The present invention also relates to the use of this method as well as to kits employing the fluorogenic dye.
Owner:QIAGEN GMBH

An organic dye, its preparation method and a sensitized solar cell containing the organic dye

The invention relates to an organic dye, a preparation method thereof and a sensitized solar cell containing the organic dye, belonging to the technical field of dye-sensitized solar cells. The organic dye has the following structure, wherein R1, R2 are independently selected from any one of H, C1~C18 alkyl, and C1~C18 alkoxy; R3, R4 are independently selected from H, C1~C18 alkyl, C1 Any one of ~C18 alkylphenyl, C1~C18 alkoxyphenyl; R5, R6 are independently selected from H, F, C1~C18 alkyl, C1~C18 alkylphenyl, C1~C18 alkoxy Any one of phenyl groups; x, y are 0 or 1. The dye of the present invention has a twisted electron skeleton, and at the same time combines side modifying groups to make it have a better three-dimensional structure. The sensitized solar cell prepared by the dye has higher voltage and can be used as a co-sensitizer in combination with other high-efficiency dyes to prepare a device with excellent performance and good photothermal stability. The preparation method of the organic dye has rich sources of raw materials, low cost, good photothermal stability of the prepared device, and bright prospect of industrial production.
Owner:CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI

Organic dye and preparation method thereof, and sensitized solar cell containing organic dye

The invention relates to an organic dye and a preparation method thereof, and a sensitized solar cell containing the organic dye, and belongs to the technical field of dye-sensitized solar cell. The organic dye has the following structure described in the description, wherein R1 and R2 are independently selected from any one of H, C1-C18 alkyl and C1-C18 alkoxy, R3 and R4 are independently selected from any one of H, C1-C18 alkyl, C1-C18 alkyl phenyl and C1-C18 alkoxy phenyl, R5 and R6 are independently selected from any one of H, F, C1-C18 alkyl, C1-C18 alkyl phenyl, and C1-C18 alkoxy phenyl, and x and y are 0 or 1. The dye has a twisted electronic skeleton and is combined with a side modifying group to make the dye have a better three-dimensional structure at the same time. The sensitized solar cell prepared by the dye has a higher voltage, and the dye can be used as a co-sensitization agent for being combined with other high-efficiency dyes to prepare a device with excellent performance and good light and thermal stability. The preparation method of the organic dye has the advantages of abundant raw material sources, lower cost, good light and thermal stability of the prepared device, and grander industrialized production prospect.
Owner:CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI

Polypeptide probe pair for measuring transpeptidase alpha activity, method for measuring transpeptidase alpha activity and application of both

Relating to the technical field of biological detection, the invention provides a polypeptide probe pair for determination of transpeptidase A activity, a determination method for the transpeptidase Aactivity and application of the two. The polypeptide probe pair for determination of the transpeptidase A activity provided by the invention comprises a first polypeptide probe and a second polypeptide probe that include a reaction zone, a complementary zone and a dye combination zone, has the advantages of strong specificity, good accuracy and high sensitivity, and can achieve the purpose of determining the transpeptidase A activity without modification or labeling treatment. The determination method for the transpeptidase A activity provided by the invention includes: mixing the polypeptideprobe pair for determination of the transpeptidase A activity with a to-be-detected sample in a transpeptidation reaction buffer solution and performing incubation, then using a biarsenical dye to label the incubated reaction product, combining the incubated reaction product with the biarsenical dye to form a compound, and judging the transpeptidase A activity according to the fluorescence levelof the compound. The method is simple and convenient to operate.
Owner:SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI

Method for improving dyeing performance of real silk fiber based on enzymatic modification

The invention discloses a method for improving dyeing performance of a real silk fabric based on enzymatic modification; serine hydroxymethyltransferase is used for catalyzing glycine in protein fibermacromolecules to be converted into serine, the number of the serine in real silk fiber is increased, phosphorylation of the serine and threonine residues in the real silk fiber is catalyzed by protein kinase A, the cationic dye binding effect is enhanced, and the dyeing performance of the real silk fabric is improved. The method specifically comprises the following steps: carrying out enzymaticconversion of amino acids in real silk; and (2) catalyzing the phosphorylation by the protein kinase A. Compared with the modification and improvement of the electronegativity of the surface of the real silk fiber by applying a chemical method, the method has the advantages of being high in enzyme catalysis efficiency, mild in reaction conditions and obvious in dyeing performance improvement.
Owner:JIANGNAN UNIV

Ring dyed polymer treated materials

Products and methods for ring dyeing a yarn and / or surface dyeing a fabric are provided. The product and method discloses the application of a dye binding composition including a urethane based polymer to a textile yarn, fabric, or garment. The dye binding composition is engineered so that the composition is positioned on or near the surface of the yarn, producing a ring dyed material. The dye binding composition can be colored with selective dyes that bind to the composition but generally do not have affinity for textile substrate. Garments created using this fabric can be abraded to remove surface polymer and color in different locations of the garment, creating an antiqued appearing garment. Dyes of different classifications can be used to produce novel performance and coloration effects in textile materials and garments. This process has been demonstrated on yarn (for a denim-like appearance), knitted and woven fabrics, and garments.
Owner:TEXSTREAM TECH

Composition and method for determining enzyme activity of M-MLV reverse transcriptase

The invention discloses an enzyme activity determination composition of M-MLV reverse transcriptase and a determination method. A fluorescent quantitative method is adopted for determining the activity of the M-MLV reverse transcriptase, and isothermal extension reaction is carried out under the condition of 37-42 DEG C. The invention also discloses a method for determining the enzyme activity of the M-MLV reverse transcriptase. According to the method, the SYBR dye is combined with the cDNA double strand to emit fluorescence, the Oligreen dye is combined with the single strand to emit fluorescence, a reaction system for determining the enzyme activity is designed, the principle that the fluorescent dye is combined with the DNA to emit fluorescence is utilized, the enzyme activity determination method of the M-MLV reverse transcriptase is designed, and compared with a traditional determination method, the time consumption is short, the operation is simple, and the repeatability is good.
Owner:苏州译酶生物科技有限公司 +1
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