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Temperature/pH double-response type chitin nano fiber hydrogel and preparation method thereof

A nanofiber, dual-response technology, which is applied in the field of nanofiber hydrogel preparation, can solve the problems of limiting the development of temperature/pH dual-response nanofiber hydrogel, complex reaction process, and many uncontrollable factors, and achieves excellent results. Effects of temperature sensitivity, simple preparation method, and strong operability

Active Publication Date: 2019-01-25
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Temperature / pH dual-responsive nanofiber hydrogels are the focus of research in the field of biomedicine. However, the current preparation process of temperature / pH dual-responsive nanofiber hydrogels mainly involves chemical grafting modification, and the reaction process is complex and uncontrollable. Many factors limit the development of temperature / pH dual-responsive nanofibrous hydrogels

Method used

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  • Temperature/pH double-response type chitin nano fiber hydrogel and preparation method thereof
  • Temperature/pH double-response type chitin nano fiber hydrogel and preparation method thereof
  • Temperature/pH double-response type chitin nano fiber hydrogel and preparation method thereof

Examples

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Effect test

Embodiment 1

[0029] (1) Preparation of Partial Chitosan

[0030] Crab shell cut into 1cm 2 Size, soak in 1mol / L NaOH for more than 12 hours to remove protein, wash with distilled water until neutral, then soak in 1mol / L HCl for more than 12 hours to remove minerals, and wash to neutral. The above steps were repeated three times. Configure 0.5% (w / w) NaClO 2 1. After adjusting the pH to 5 with acetic acid, bleach in a water bath at 70°C for 2 hours, and stir intermittently to wash the chitin until neutral. Crushed by a juicer, filtered to remove water, stored in a refrigerator at 4°C, and balanced for 1-2 days.

[0031] 1 g of purified chitin (dry weight) was weighed, soaked in 50 mL of 35% (w / w) NaOH solution, stirred in a water bath at 90° C. for 4 h for partial deacetylation treatment. After the reaction was over, it was washed to neutrality and the solid was collected. Store in the refrigerator at 4°C, with water balance.

[0032] (2) Preparation of chitin nano-dispersion

[0033...

Embodiment 2

[0042] (1) Preparation of Partial Chitosan

[0043] The squid parietal bone was soaked in 1mol / L NaOH for more than 12 hours to remove protein, washed with distilled water until neutral, then soaked in 1mol / L HCl for more than 12 hours to remove minerals, and washed to neutral. The above steps were repeated three times. Configure 0.5% (w / w) NaClO 2 1. After adjusting the pH to 5 with acetic acid, bleach in a water bath at 70° C. for 2 hours, stir intermittently, and wash the chitin until neutral. Crushed by a juicer, filtered to remove water, stored in a refrigerator at 4°C, and balanced for 1-2 days.

[0044] 1 g of purified chitin (dry weight) was weighed, soaked in 50 mL of 35% (w / w) NaOH solution, stirred in a water bath at 90° C. for 4 h for partial deacetylation treatment. After the reaction was over, it was washed to neutrality and the solid was collected. Store in the refrigerator at 4°C, with water balance.

[0045] (2) the preparation of chitin nano-dispersion i...

Embodiment 3

[0053] (1) Preparation of Partial Chitosan

[0054] Cicada molting shells were soaked in 1mol / L NaOH for more than 12 hours to remove protein, washed with distilled water until neutral, then soaked in 1mol / L HCl for more than 12 hours to remove minerals, and washed until neutral. The above steps were repeated three times. Configure 0.5% (w / w) NaClO 2 1. After adjusting the pH to 5 with acetic acid, bleach in a water bath at 70°C for 2 hours, and stir intermittently to wash the chitin until neutral. Crushed by a juicer, filtered to remove water, stored in a refrigerator at 4°C, and balanced for 1-2 days.

[0055] 1 g of purified chitin (dry weight) was weighed, soaked in 50 mL of 35% (w / w) NaOH solution, stirred in a water bath at 90° C. for 4 h for partial deacetylation treatment. After the reaction was over, it was washed to neutrality and the solid was collected. Store in the refrigerator at 4°C, with water balance.

[0056] (2) Preparation of chitin nano-dispersion

...

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Abstract

The invention discloses temperature / pH double-response type hydrogel and a preparation method thereof. The method comprises the following steps: preparing partial chitosan; preparing a chitin nano fiber dispersion; slowly dropwise adding and mixing a water phase, an oil phase and a surfactant according to a proportion, and continuously stirring for 2-72 hours to obtain a clear transparent oil-in-water microemulsion; uniformly mixing the chitin nano fiber dispersion, the microemulsion, beta-phosphoglycerol and a biological complex factor according to a proportion, and performing alkaline coagulating bath to obtain the temperature / pH double-response type hydrogel. In the invention, the temperature / pH double-response type hydrogel is prepared by compounding the beta-GP and the biological complex factor with the chitin nano fiber, and the preparation method is simple and has strong operability. The temperature / pH double-response type hydrogel has good mechanical property, biocompatibility,temperature responsiveness, pH responsiveness and slow release property, and can be applied to the medical fields such as slow release of drugs, and thus has a good application prospect.

Description

technical field [0001] The invention relates to the field of nanofiber hydrogel preparation, in particular to a temperature / pH double-responsive chitin nanofiber hydrogel and a preparation method thereof. Background technique [0002] Chitin is a natural high molecular polymer linked by β-(1-4) glycosidic bonds, which can be dispersed in weak acid solution after partial deacetylation treatment. There are enough free amino groups on the surface of chitin fiber crystals and high-density positive charges on the surface under acidic conditions, and nanofibers can be obtained after certain mechanical treatment. Chitin nanofibers have particularly excellent biological properties, and the sufficient number of amino groups on the surface make them have better biological and chemical reaction properties, and can be widely used in biomedical products, drug delivery systems, and so on. With the improvement of the utilization rate of some expensive and insoluble drugs and the increasin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L5/08C08L3/02C08L5/14C08K5/521C08K5/17C08J3/075A61K47/36A61K47/24A61K47/18
CPCA61K47/183A61K47/24A61K47/36C08J3/075C08J2305/08C08J2403/02C08J2405/08C08J2405/14C08K5/17C08K5/521
Inventor 范一民王蓉俞娟王志国刘亮
Owner NANJING FORESTRY UNIV
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