Cuttage method of graptopetabum paraguayense
A gem and stuffy shed technology, applied in gardening methods, botanical equipment and methods, gardening, etc., can solve the problems of a large number of rotten leaves and a low survival rate, and achieve the effect of improving the survival rate and shortening the root differentiation time.
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Embodiment 1
[0013] 1. Select a healthy and vigorous gemstone flower, cut off the single leaf, remove the lower leaf, rinse with clean water for 12 hours, disinfect with 70% alcohol for 30 seconds on an ultra-clean workbench, then rinse with sterile water for 3 times, and use 0.1% mercury liter Disinfect the water solution twice, each time for 4 minutes, then rinse with sterile water for 3 times, take it out and blot the water with sterile filter paper, the incision should be dry, and then apply 1000ppm of naphthaleneacetic acid to the incision;
[0014] 2. Cut the sterilized shoots into sections containing buds and place them horizontally in the basic medium, and cultivate them for 25 days to induce germination of young shoots or cluster buds; transfer the induced young shoots or cluster buds to the subculture medium medium, cultivated for 25 days;
[0015] 3. Open the bottle and harden the rooted seedlings in the greenhouse for 2 days, remove the subculture medium at the base, and transp...
Embodiment 2
[0017] 1. Select a healthy and vigorous gemstone flower, cut off the top branch, remove the lower leaves, rinse with water for 2 hours, disinfect with 70% alcohol for 30 seconds on an ultra-clean workbench, then rinse with sterile water for 3 times, and use 0.1% mercury chloride Disinfect the water solution twice, each time for 4 minutes, then rinse with sterile water for 3 to 5 times, take it out and blot the water with sterile filter paper, the incision should be dry, and then apply 1000ppm of naphthaleneacetic acid to the incision;
[0018] 2. Cut the sterilized shoots into sections containing buds and place them horizontally in the basic medium, and cultivate them for 30 days to induce germination of young shoots or cluster buds; transfer the induced young shoots or cluster buds to the subculture medium medium, cultivated for 30 days;
[0019] 3. Open the bottle and harden the rooted seedlings in the greenhouse for 3 days, remove the subculture medium at the base, and tran...
Embodiment 3
[0021] 1. Select a healthy and vigorous gemstone flower, cut off the single leaf, remove the lower leaf, rinse it with water for 1.5 hours, disinfect it with 70% alcohol on the ultra-clean workbench for 30 seconds, then rinse it with sterile water for 3 times, and rinse it with 0.1% L Sterilize with mercury aqueous solution for 2 times, each time for 4 minutes, then rinse with sterile water for 4 times, take it out and dry it with sterile filter paper, the cut should be dry, and then apply 1000ppm of naphthaleneacetic acid to the cut;
[0022] 2. Cut the sterilized shoots into sections containing buds and place them horizontally in the basic medium, and cultivate them for 28 days to induce germination of young shoots or cluster buds; transfer the induced young shoots or cluster buds to the subculture medium medium, cultivated for 28 days;
[0023] 3. Open the bottle and harden the rooted seedlings in the greenhouse for 2 days, remove the subculture medium at the base, and tran...
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