Biological 3d printed active biological membrane for improving AMIC technology cartilage repair and preparation method thereof

Abstract

The invention discloses a biological 3d printed active biological membrane of improved AMIC for cartilage repair. The method is characterized in that: sodium alginate, gelatin and hyaluronic acid areused as raw materials to prepare mixed hydrogel, cartilage precursor cells and fibronectin are mixed into the hydrogel, a deposition type biological 3D printing technology is used for constructing theporous hydrogel biological membrane, and chemical crosslinking is achieved through calcium chloride soaking to enhance the mechanical property. The materials used in the invention are all natural materials, are low in immunogenicity, good in biocompatibility and wide in source, have certain mechanical properties and can provide good support for cartilage regeneration. In terms of the preparationmethod, gaps of 300-500 microns can promote cartilage regeneration, meanwhile, an excellent porosity structure and the like can also promote intercellular substance exchange and communication, facilitate adhesion of cytokines or cells is, and facilitate growth and proliferation of the cells in the structure.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a bio-3D-printed modified AMIC active biofilm for cartilage repair and a preparation method thereof. Background technique [0002] Because the hyaline cartilage of the joint lacks the control of blood vessels, nerves, and lymph, it is difficult to achieve self-healing and easily develop into degenerative diseases once damaged. The existing clinical treatment techniques such as osteochondral transplantation, autologous chondrocyte transplantation, microfracture technology and Restorative effects such as AMIC technology are limited and the long-term curative effect is not good. In recent years, tissue engineering technology and bio-3D printing technology are gradually becoming a new generation of cartilage repair technology. 3D printing technology can precisely control the internal structure of the scaffold, construct a structure similar to cartilage morphology, and c...

AI Technical Summary

Problems solved by technology

[0006] For large-scale talus osteochondral injuries, the most common method at present is to use allogeneic bone transplantation after the lesion is removed. When the allogeneic bone is taken, the three-dimensional geometry of the amputated recipient's talus lesion is artificially observed and the corresponding part is cut from the allogeneic talus. For repair and reconstruction, this method has strong subjectivity and inaccuracy, and the shape of the talus is irregular, and the size, shape, and radian of each person's talus are not the same. The obtained allogeneic bone cannot be accurately matched and reconstructed with the recipient's talus , the size, shape, and radian of the cut allograft bone and the recipient talus are likely to be inconsistent, and the articular surface is uneven, resulting in abnormal stress in the joint after surgery, osteoarthritis, etc., resulting in poor curative effect or surgical failure. Therefore, a Cartilage 3D printer capable of precise repairs

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