30 results about "Anaphylactoid reactions" patented technology

The invention belongs to the field of medicament detection, and relates to a method for detecting materials causing anaphylactoid reaction. The method comprises the following steps of: culturing cells and medicaments together; scanning images of acquired culture by a microscopic; and determining the existence of allergy substances according to change of cell morphology in images as well as the change of calcium ions in the cells.

The invention belongs to the field of medicines, and particularly relates to a method for analyzing quality safety of a traditional Chinese medicine injection and a medicine preparation. According to the invention, the traditional Chinese medicine injection serves as an object of study, a Qingkailing injection, a Chuanhuning injection and a Shenmai injection serve as representatives, a mast cell membrane stabilizer and other anti-allergic medicines are added into the traditional Chinese medicine injection to inhibit various immune toxicity reactions induced by the traditional Chinese medicine injection, and the capability of inducing various hypersensitivities and anaphylactoid reactions is detected, so that the result proves the feasibility of the method for quality control of immunity safety of the traditional Chinese medicine injection. The method provides a novel solution for the main adverse reaction, namely immunotoxicity, of the traditional Chinese medicine injection applied to clinic. A method for detecting the immunity safety control of the traditional Chinese medicine injection is established to control the clinical adverse reactions of the traditional Chinese medicine injection; the mechanism of action is studied, so that the selection and basis are provided for the method for detecting the immunity safety control of the traditional Chinese medicine injection in the new version of pharmacopeia, and the reference is provided for establishing novel guiding principles of immunity safety evaluation for new drugs in China.

The invention belongs to the field of pharmaceutical preparations, and in particular relates to an allergy-like testing method for Ciwujia injection. The test method provided by the invention comprises the following steps: (1), sample pretreatment; (2), preparation of detection cells; (3), detection of release rate of β-hexosaminidase. Compared with the prior art, the anaphylactoid test method of Acanthopanax injection provided by the present invention is simple to operate, and the test time is short; it makes the evaluation and judgment of the test results more convenient, intuitive and reliable; using the Acanthopanax provided by the present invention The anaphylactoid test method for injection can obtain relatively accurate test and judgment results that are consistent with the actual mouse experiment; it is more stable and reliable, there is no obvious difference in multiple test results for the same sample, and the repeatability is high.

The invention relates to a ginkgo leaf composition, in particular to a ginkgo leaf composition and application thereof for preparing a ginkgo biloba leaf extract injection. The ginkgo leaf composition is prepared from 25-43% of total flavonol glycosides, 6.5-17% of ginkgolides, 3-5% of bilobalide and smaller than 3 ppm of ginkgoic acid. According to the preparation method of the ginkgo leaf composition, two kinds of resin of DM130 and AB-8 are adopted for adsorption, the harmful ingredient total ginkgolic acid is effectively removed, and anaphylactoid reaction occurrences are reduced; meanwhile, various extracting methods and composite extracting agent repeated extraction are adopted, the target component content is high, the purity is good, and the product quality is improved. The obtained ginkgo biloba leaf extract injection is clear in compositional ratio, good in stability, good in solubility and stable in curative effect and contains few impurities.

The invention discloses a polypeptide with immunogenicity for anaphylactoid specific receptor MRGPRX2 protein and application thereof, belonging to the technical field of biomedicine. The polypeptide consists of 15 amino acids, and its polypeptide sequence is: WKDSDVLFCHIHPVS. The epitope of the polypeptide is the 250-264th amino acid sequence of MRGPRX2 protein. It is found through research that it has strong immunogenicity. Therefore, it can be used for the preparation of mouse monoclonal antibody and rabbit polyclonal antibody, and successfully prepared a double-antibody sandwich ELISA method, in which the monoclonal antibody is the coating antibody and the polyclonal antibody is the detection antibody, which verifies that the established method can be used in clinical testing. This will help the research work on the specific receptor MRGPRX2 of the anaphylactoid response, and is of great significance for guiding the safety of drug use in clinical practice.

The invention provides application of furocoumarins compound in preparing anti-allergic medicine and belongs to the technical field of biological medicine. The application disclosed by the invention proves that the furocoumarins compound can effectively antagonize beta-hexosaminidase release caused by C48 / 80 of human mast cell KU812 for the first time and further can inhibit anaphylactoid reaction; furthermore, the application also proves that an action receptor of the furocoumarins compound is an MrgprX2 receptor for the first time; when the furocoumarins compound in preparing anti-allergic medicine is prepared into anti-allergic preparation, anti-allergic medicine types can be enriched, and a brand-new choice and a brand-new strategy are provided for anti-allergic treatment.

The invention relates to the technical field of drug testing, in particular to a method for detecting a substance inducing anaphylactoid reaction and a kit thereof. The method comprises the following steps: inoculating P815 cells into a culture medium; adding a to-be-detected object and a biological molecule specifically bonded with IP1 and / or cAMP to the culture medium, and detecting concentration of IP1 and / or cAMP; and according to the concentration of IP1 and / or cAMP, determining whether the to-be-detected object is the substance inducing the anaphylactoid reaction or not. The detection method provided by the invention has the advantages that the result is accurate and reliable and is basically consistent with the detection result of the traditional beta-hexosaminidase release rate experiment, but the sensitivity is higher than that of a beta-hexosaminidase release rate detection method; and the detection method provided by the invention is simple and quick, overcomes the defects of the traditional method, can be applied to early stage rapid screening and identification of the substance inducing the anaphylactoid reaction and has extensive value in use.

The invention relates to a ginkgo leaf composition, and in particular relates to a Shuxuening injection and a preparation method thereof. The Shuxuening injection is made from ginkgo leaf extract which contains 25-40% of total flavonol glycosides, 6.5-16% of ginkgolide, 3-5% of bilobalide and less than 5ppm of ginkgolic acid. The preparation method has the advantages that a harmful ingredient, namely total ginkgolic acid, is effectively removed, and anaphylactoid reactions are reduced. The prepared Shuxuening injection is definite in compositional ratio, good in stability, low in irritability, good in solubility and stable in curative effect.

The invention discloses a polypeptide for preparing rabbit polyclonal antibody and its application, belonging to the technical field of biomedicine. The polypeptide is an immunogenic polypeptide similar to the allergic response specific receptor MRGPRX2 protein, and can be used to prepare rabbit polyclonal antibodies. The enzyme-linked immunosorbent method (direct method) has been successfully prepared, wherein the polyclonal antibody is a detection antibody. The established method has verified its clinical application, which will help the research work on the specific receptor for anaphylactoid MRGPRX2, and is of great significance for clinically guiding the safety of drug use.

The invention discloses a method for detecting anaphylactoid reaction caused by chlorogenic acids. The method comprises the following steps of (1) respectively preparing RBL-2H3 cell suspension and chlorogenic acid solution which are different in concentration; (2) determining the optimal cell concentration of the RBL-2H3 cell suspension and the optimal time point of addition of the chlorogenic acid solution when a real-time cell analyzer detects the anaphylactoid reaction caused by chlorogenic acids; and (3) detecting the RBL-2H3 cell growth index values before and after addition of the chlorogenic acid solution by utilizing a real-time cell analyzer. The method has the advantages that growth, extension, morphologic change and death physiological states of the RBL-2H3 cell before and after the chlorogenic acid solution is added can be reflected in real time; and the concentration range of the solution of the chlorogenic acids causing the anaphylactoid reaction can be obtained.