The invention discloses total nutrient formula powder as well as a preparation method and application thereof, and belongs to the field of foods. The total nutrient formula powder specifically comprises the following components in parts by weight: 0.5-3.5 parts of protein, 0.4-2.0 parts of fat, 1.8-6.0 parts of carbohydrate, 0.05-0.5 part of compound vitamins, 0.05-0.5 part of compound minerals, 0.1-1.0 part of dietary fibers and 0.05-1.0 part of probiotics. Through the design, screening and scientific proportioning of raw materials and auxiliary materials, nutrient substances are promoted to be comprehensive and balanced, human body absorption is facilitated, and the total nutrient formula powder is scientific in formula, good in liquidity and reconstituability, convenient to eat, good in taste and the like, can serve as a single nutrient source to meet the nutritional requirements of people with limited feeding, digestion and absorption disorders, metabolic disorders or specific disease states, besides, can promote the digestive system to be healthier, can also be used as a nutritional supplement, and has wide popularization and application market prospects.