Preparation method for detection reagent box of sore mouth disease antibody
A technology for antibody detection and kits, which is applied in the field of preparation of aphthous ulcer antibody detection kits, can solve the problems that the prevalence of aphthous ulcers cannot be explained, cannot be widely promoted, and the operation is complicated, so as to prevent false positive results and achieve good results. Detection, high sensitivity effect
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[0014] The preparation of the oral ulcer antibody detection kit includes the following steps: 1) isolation and preliminary culture of oral ulcer virus; 2) cloning and prokaryotic expression of the early expression protein genes B2L, F1L, VIR, and CBP of oral ulcer virus; 3) prokaryotic expression Protein and oral ulcer virus cell culture fluid are respectively used as coating antigens to detect the serum samples to be tested, and the detection effect of which protein is determined by comparison, so as to determine the best coating antigen, as a detection kit, to determine its infection status and virus popular features. (4) The establishment of a mouth ulcer antibody detection kit and the specificity, sensitivity and repeatability tests.
[0015] Among them, the selected gene is the early expression protein gene of the aphthus virus, which is unique to the aphthus virus, and begins to be expressed in the early stage of infection, and the similarity between different strains is...
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