Preparation method of ginkgo leaf extract

A technology of ginkgo biloba and extract, which is applied in the field of medicine, can solve problems such as waste of resources, and achieve the effects of saving time, ensuring continuity, and improving product quality

Inactive Publication Date: 2016-01-27
HEILONGJIANG ZBD PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the technical scheme provided by this invention can produce the ginkgo leaf extract / thing that meets the requirements of the quality standard, there are still ginkgolic acids in the obtained ginkgo leaf extract / thing, and high-porous resin column purification process is used. Concentrated ethanol (60-80%), resulting in a waste of resources

Method used

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  • Preparation method of ginkgo leaf extract
  • Preparation method of ginkgo leaf extract
  • Preparation method of ginkgo leaf extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Take 1 kg of ginkgo leaf slices, crush them, pass through an 8-mesh sieve, add 70% ethanol that is 10 times the weight of ginkgo leaves (v / w), soak for 2 hours, heat and reflux at 70°C for 2 hours, and filter with 3 layers of medium-speed filter paper , to obtain medicinal residues and liquid extraction;

[0029] (2) Add 70% ethanol with 8 times the weight of ginkgo leaves (v / w) to the dregs obtained in step (1), heat and reflux at 70°C for extraction for 2 hours, and filter with 3 layers of medium-speed filter paper to obtain dregs and extract ;

[0030] (3) Combine the extracts obtained in steps (1) and (2). When the temperature drops to 30°C, adjust the pH to 7.9 with 20% NaOH solution, filter with 3 layers of medium-speed filter paper, and concentrate the filtrate at 70°C under vacuum rotation Recover the ethanol and concentrate it to 1 g ginkgo leaf / mL, add purified water to 10 times the weight of the ginkgo leaf (v / w), adjust the pH to 2.0 with HCl at 35°C, a...

Embodiment 2

[0034] (1) Take 1 kg of ginkgo leaf slices, crush them, pass through an 8-mesh sieve, add 75% ethanol that is 10 times the weight of ginkgo leaves (v / w), soak for 3 hours, heat and reflux at 60°C for 3 hours, and filter with 3 layers of medium-speed filter paper , to obtain medicinal residues and liquid extraction;

[0035] (2) Add 75% ethanol with 8 times the weight of ginkgo leaves (v / w) to the dregs obtained in step (1), heat and reflux at 60°C for extraction for 2 hours, and filter with 3 layers of medium-speed filter paper to obtain dregs and extract ;

[0036] (3) Combine the extracts obtained in steps (1) and (2). When the temperature drops to 40°C, adjust the pH to 8.1 with 20% NaOH solution, and place it in refrigeration for 12 hours. After refrigeration, precipitation occurs. Filtrate with fast filter paper, concentrate the filtrate at 60°C under vacuum rotation to recover ethanol, and concentrate to 2 g ginkgo leaves / mL, add purified water to 5 times the weight of ...

Embodiment 3

[0040] (1) Take 1 kg of ginkgo leaf slices, crush them, pass through an 8-mesh sieve, add 60% ethanol that is 10 times the weight of ginkgo leaves (v / w), soak for 2 hours, heat and reflux at 68°C for 2 hours, and filter with 3 layers of medium-speed filter paper , to obtain medicinal residues and liquid extraction;

[0041] (2) Add 60% ethanol with 8 times the weight of ginkgo leaves (v / w) to the dregs obtained in step (1), heat and reflux at 70°C for extraction for 1 hour, filter with 3 layers of medium-speed filter paper, and obtain dregs and extract ;

[0042] (3) Combine the extracts obtained in steps (1) and (2). When the temperature drops to 35°C, adjust the pH to 8.0 with 20% NaOH solution, and place it in refrigeration for 24 hours. After refrigeration, precipitation occurs. Filtrate with fast filter paper, concentrate the filtrate at 68°C with a vacuum scraper to recover ethanol, and concentrate to 3g ginkgo leaves / mL, add purified water to 10 times the weight of gin...

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Abstract

The invention discloses a preparation method of a ginkgo leaf extract. The preparation method comprises the following steps: (1) heating for reflux extraction by using ethanol; (2) adjusting the pH to be alkaline, and placing for cold storage; (3) passing through a macroporous resin column; and (4) filtering through a microporous filtering film, and sterilizing to obtain the ginkgo leaf extract, wherein 25% ethanol and a 10% sodium acetate solution are used for eluting impurity peaks in the step (3), and 30-50% ethanol is used for eluting target component peaks. The preparation method disclosed by the invention has the beneficial effects that a harmful component namely total ginkgolic acid is effectively removed, anaphylactoid reaction is reduced, the safety is improved, the purity of a target component is improved, the product quality is improved, and automatic online detection is realized.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method of ginkgo leaf extract. Background technique [0002] Ginkgo biloba is the leaf of Ginkgo biloba (Ginkgobiloba), which is one of the oldest tree species on the earth today. Ginkgo biloba is a Ginkgo biloba plant (Ginkgobiloba.L.), also known as Gongsun tree. It is a special plant in my country. Ginkgo biloba extract (GRE) mainly contains two types of active ingredients, flavonoids and lactones. The flavonoids are kaempferal (kaempferal), Quercetin (quercetin) and isorhamnetin (isorhamnetin) and other glycosides are the main compounds, and the lactones are mainly bilobalide and ginkgolides A, B and C (gimkgolideA.B.C). Due to the definite curative effect of Ginkgo biloba extract in the treatment of coronary heart disease, cerebral thrombosis, cerebral ischemia, brain dysfunction, sequelae of brain trauma, and oxygen free radical scavenging in nervous system diseases,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16
Inventor 方同华许照芹项彦华席桂才李学东岳大彪周广红贾文娟
Owner HEILONGJIANG ZBD PHARMA
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