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Cell detection kit and application thereof

A technology for detecting kits and cells, applied in measuring devices, instruments, fluorescence/phosphorescence, etc., can solve problems such as waste of dyes, high cost of reagents, inaccurate detection and counting of nucleated red blood cells, etc.

Active Publication Date: 2019-08-16
SHENZHEN DYMIND BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a cell detection kit and its application, which solves the problems of inaccurate detection and counting of nucleated red blood cells, waste of dyes, inability to choose flexibly, and high cost of reagents in the prior art

Method used

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  • Cell detection kit and application thereof
  • Cell detection kit and application thereof
  • Cell detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Prepare a solution with the following components:

[0083] Staining solution:

[0084] Nucleic acid dye 0.12mmol of the compound shown in formula V;

[0085] Ethylene glycol 1000mL;

[0086] Hemolytic agent:

[0087] Dodecyl dimethylol betaine 2.0g;

[0088] Decyl polyoxyethylene ether 3.0g;

[0089] Octyltrimethylammonium bromide 1.0g;

[0090] Formic acid 3.0g;

[0091] Sodium formate 3.5g

[0092] Proclin300 0.2g;

[0093] 1L of water;

[0094] The pH was adjusted to 2.2.

[0095] where X - for Cl - .

[0096] Mix the nucleic acid dye of formula V with ethylene glycol according to the above ratio, stir at room temperature until completely dissolved, filter with a 0.2 μm filter membrane, and store in a sealed and dark place.

[0097] Mix lauryl dimethylol betaine, lauryl polyoxyethylene ether, octaalkyltrimethylammonium bromide, formic acid, sodium formate, and Proclin300 according to the above ratio, add deionized water to 1L, and stir at room temperat...

Embodiment 2

[0099] Prepare a solution with the following components:

[0100] Staining solution:

[0101]Formula VI nucleic acid dye 0.095mmol;

[0102] Methanol 1000mL;

[0103] Hemolytic agent:

[0104] Tetradecyl dimethyl betaine 0.3g;

[0105] Fatty alcohol polyoxyethylene ether (AEO-9) 4.0g;

[0106] Decyltrimethylammonium bromide 5.0g;

[0107] Citric acid 2.5g;

[0108] Sodium citrate 3.0g;

[0109] Phenoxyethanol 0.5g;

[0110] 1L of water;

[0111] The pH was adjusted to 2.5.

[0112] where X - for Br - .

[0113] Mix the above nucleic acid dye with methanol according to the above ratio, stir at room temperature until completely dissolved, filter with a 0.2 μm filter membrane, and store in a sealed and dark place.

[0114] Mix myristyl dimethyl betaine, fatty alcohol polyoxyethylene ether (AEO-9), dodecyltrimethylammonium bromide, citric acid, sodium citrate, and phenoxyethanol in the above ratio, and add Make up to 1L of ionized water, stir at room temperature un...

Embodiment 3

[0116] Prepare a solution with the following components:

[0117] Staining solution:

[0118] Formula VII nucleic acid dye 0.250mmol;

[0119] Ethylene glycol 900mL;

[0120] Propanol 100mL.

[0121] Hemolytic agent:

[0122] Sodium hydroxymethylglycinate 3.5g;

[0123] Lauryl polyoxyethylene ether 2.8g;

[0124] Sodium lauryl sulfate 1.3g;

[0125] Formic acid 2.5g;

[0126] Sodium formate 2.0g;

[0127] Sorbic acid 0.5g;

[0128] 1L of water;

[0129] The pH was adjusted to 2.8.

[0130] where X - for Br - .

[0131] Mix the nucleic acid dye of formula VII with ethylene glycol and propanol according to the above ratio, stir at room temperature until completely dissolved, filter with a 0.2 μm filter membrane, seal and store away from light.

[0132] Mix sodium hydroxymethylglycinate, lauryl polyoxyethylene ether, sodium lauryl sulfate, formic acid, sodium formate, and sorbic acid in the above ratio, add deionized water to 1L, stir at room temperature until com...

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Abstract

The invention relates to a cell detection kit and application thereof. The cell detection kit includes a staining solution and a hemolytic agent. Nucleated red blood cells, basophilic granulocyte andother four kinds of white blood cells are distinguished through simultaneous usage of reagents in the cell detection kit, the nucleated red blood cells and the basophilic granulocyte can be accuratelydetermined, the basophilic granulocyte and the other four kinds of white blood cells can be distinguished through individual usage of the hemolytic agent, and the basophilic granulocyte can be accurately determined. Therefore, according to the cell detection kit, the basophilic granulocyte and / or the nucleated red blood cells and other white blood cells can be classified and counted through autonomous selection of modes.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, more specifically, to a cell detection kit and its application. Background technique [0002] Nucleated erythrocytes are immature erythrocytes, which are a type of red blood cells. Normally, a small amount of nucleated erythrocytes can be seen in blood smears of infants and young children within 1 week, while adult nucleated erythrocytes are all present in bone marrow, as seen in peripheral blood smears It is a pathological phenomenon, including: primitive red blood cells, early young red blood cells, middle young red blood cells and late young red blood cells. There is also a nucleus in nucleated red blood cells, and the larger the nucleus, the more immature the cell. Determination of nucleated red blood cells is of clinical importance. [0003] The traditional identification and counting method of nucleated red blood cells is to count the number of nucleated red blood cells in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6439Y02A50/30
Inventor 余晓尘舒家发李春晖伍耀婵崔晓磊辛佩轩其他发明人请求不公开姓名
Owner SHENZHEN DYMIND BIOTECH
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