Test method for basophilic granulocyte activation and degranulation

Abstract

The invention discloses a test method for basophilic granulocyte activation and degranulation. The method comprises: diluting a to-be-tested allergen to prepare a standard to-be-tested allergen, setting a positive control and a negative control, wherein all to-be-detected samples are heparinized peripheral blood of a patient and need to be sealed and incubated, then adding a buffer solution, centrifuging, adding a fluorescent dyeing mixed solution, lightly mixing until precipitate is suspended, incubating in a dark place, adding an erythrocyte lysate, uniformly mixing in a vortex manner, incubating in a dark place, diluting, centrifuging, re-suspending the precipitate, adding a buffer solution after filtering, and detecting by adopting a flow cytometer to obtain an activation and degranulation ratio. According to the method, the basophilic granulocyte is not required to be separated and purified, the new specific basophilic granulocyte marker combination is selected, the use amount ofthe detected sample is small, the repeatability is good, the error is small, the activation and degranulation degrees of the basophilic granulocyte are effectively distinguished, and the detection andthe technical support are well provided for the clinical differential diagnosis and the scientific research project of related diseases.

Description

technical field [0001] The invention belongs to the technical field of allergic disease diagnosis, and in particular relates to a test method for basophil activation and degranulation. Background technique [0002] With the increasingly serious global environmental pollution, chemical abuse, ionizing radiation, genetically modified food, and various food additives, a series of problems continue to appear, "allergies" have become another big "killer" of human health . According to reports, 30%-40% of the world's people are plagued by allergic problems, involving multiple organs and systems, such as skin allergic reactions, respiratory allergic reactions, gastrointestinal allergic reactions, and anaphylactic shock. According to epidemiological data in 2018, 300 million people worldwide suffer from asthma, 400 million people suffer from eczema and atopic dermatitis, and more than 500 million people suffer from allergic rhinitis. The natural process of allergic diseases is tha...

AI Technical Summary

Problems solved by technology

Unfortunately, the practical results show that the combination of commonly used biomarkers makes the accuracy, sensitivity and specificity of BAT detection vary greatly among different individuals. In addition, the level of peripheral blood basophils in some allergic patients themselves Therefore, BAT has not been included in routine clinical detection methods at present, but only used in some clinical research

Images