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Transcriptome expression profile of activated basophilic granulocyte and construction method and application thereof

A technology of basophils and construction methods, which is applied in the field of transcriptome expression profile and construction of basophils, and can solve the problems that there are no basophils

Pending Publication Date: 2020-06-19
AFFILIATED HOSPITAL OF GUANGDONG MEDICAL UNIV
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  • Claims
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AI Technical Summary

Problems solved by technology

At present, there is no report on the identification of transcriptomic expression profiles related to basophil activation

Method used

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  • Transcriptome expression profile of activated basophilic granulocyte and construction method and application thereof
  • Transcriptome expression profile of activated basophilic granulocyte and construction method and application thereof

Examples

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Embodiment 1

[0030] An embodiment of the method for constructing the transcriptome expression profile of activated basophils in the present invention, including the preparation, activation and culture of human basophils, extraction of total cellular RNA, whole transcriptome sequencing, whole Identification of transcriptome expression profiles.

[0031] The human peripheral blood basophils before activation provided in this example are grouped into Control 1, Control2, and Control 3, and the corresponding human peripheral blood basophils after activation are grouped into Anti-IgE 1, Anti- IgE2, Anti-IgE3.

[0032] in, Preparation, activation and culture of human basophils , including the following steps:

[0033] (1) Specimen collection: After obtaining informed consent from healthy volunteers, peripheral venous blood was collected in EDTA anticoagulant tubes for future use.

[0034] (2) Preparation of polymorphonuclear cells: place anticoagulated blood in a 15ml centrifuge tube, add Hetas...

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Abstract

The invention discloses a construction method of a transcriptome expression profile of activated basophilic granulocyte. The construction method comprises the following steps of: preparing, activatingand culturing basophilic granulocyte, extracting total RNA of cells, sequencing a whole transcriptome and identifying a whole transcriptome expression profile. The invention further discloses the transcriptome expression profile of the activated basophilic granulocyte; compared with a transcriptome expression profile of non-activated human basophilic granulocyte, 6 remarkable up-regulation effects exist in mRNA (messenger ribonucleic acid), 10 remarkable up-regulation effects exist in lncRNA (long non-coding ribonucleic acid), 6 remarkable up-regulation effects exist in circRNA (circular ribonucleic acid) and 8 remarkable up-regulation effects exist in microRNA. According to the construction method disclosed by the invention, the change of the transcriptome expression profile related to the activation of the human basophilic granulocyte is systematically researched and a plurality of RNA which are differentially expressed after being activated are identified; and the RNA provides a new target for inhibiting the activation of the human basophilic granulocyte, so that a new direction is provided for treating allergic diseases and autoimmune diseases.

Description

technical field [0001] The invention relates to the technical field of transcriptome, in particular to a transcriptome expression profile of activated basophils and its construction method and application. Background technique [0002] Basophils are a type of peripheral blood leukocytes, mainly composed of bone marrow CD34 + derived from hematopoietic progenitor cells. An increase in the number of basophils is common in certain allergic diseases, blood diseases, tumors, etc.; a decrease in the number of basophils is common in type I hypersensitivity reactions (immediate allergic reactions), such as urticaria, anaphylactic shock, etc. . At present, it is generally believed that basophils are one of the main effector cells of allergic diseases and play an important role in allergic diseases such as asthma. [0003] Activation is a key step for basophils to exert their biological functions. In different disease states, IgE antibodies in the body are elevated and bind to the...

Claims

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Application Information

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IPC IPC(8): C12Q1/6811A61K45/00A61P37/08A61P37/02
CPCA61K45/00A61P37/02A61P37/08C12Q1/6811C12Q2535/122C12Q2539/103
Inventor 潘庆军刘华锋陈小群廖淑珍陆兴招春飞杨陈
Owner AFFILIATED HOSPITAL OF GUANGDONG MEDICAL UNIV
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