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Paper based sensor capable of detecting allergic reactions as well as preparation and application of paper based sensor

An allergic reaction and sensor technology, applied in instruments, measuring devices, scientific instruments, etc., can solve complex and expensive problems, achieve high-precision detection, convenient operation, and improved sensitivity

Inactive Publication Date: 2018-12-07
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The current methods for detecting allergic reactions are relatively complicated and expensive, so a simple, low-cost and rapid detection method is needed to judge allergic reactions

Method used

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  • Paper based sensor capable of detecting allergic reactions as well as preparation and application of paper based sensor
  • Paper based sensor capable of detecting allergic reactions as well as preparation and application of paper based sensor
  • Paper based sensor capable of detecting allergic reactions as well as preparation and application of paper based sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A paper-based sensor capable of detecting allergic reactions, the preparation method of which is as follows:

[0040] 1.1 Synthesis of Ni-NTA:

[0041] (1) NTA: At 0°C, add 1.05 g of bromoacetic acid into a round bottom flask, and dissolve it with 8 mL of sodium hydroxide. Dissolve 1g of N-carbobenzyloxylysine in 10mL of sodium hydroxide, then add it into the flask, mix well, stir at room temperature for 2h, and then heat to 50°C for 19h. At 0°C, 5 mL of concentrated hydrochloric acid was added to the reacted solution to form a precipitate, which was filtered under reduced pressure to form a white powder. Dissolve 2 g of the obtained white powder in 25 mL of methanol, add 100 mg of palladium carbon, fill the flask with hydrogen, stir the reaction mixture at room temperature for 12 h, filter the solution under reduced pressure to remove palladium carbon to obtain NTA, the reaction formula is as follows:

[0042]

[0043] (2) Ni-NTA: Take 1mg of NTA and 5mL of NiCl 2 ...

Embodiment 2

[0058] A paper-based sensor capable of detecting allergic reactions, the preparation method of which is as follows:

[0059] 1.1 Synthesis of Ni-NTA:

[0060] (1) NTA: At 0°C, add 2.1 g of bromoacetic acid into a round bottom flask, and dissolve it with 4 mL of sodium hydroxide. Dissolve 0.5g of N-carbobenzyloxylysine in 10mL of sodium hydroxide, then add it into the flask, mix well, stir at room temperature for 2h, and then heat to 50°C for 19h. At 0°C, 10 mL of concentrated hydrochloric acid was added to the reacted solution to form a precipitate, which was filtered under reduced pressure to form a white powder. Dissolve 0.5 g of the obtained white powder in 50 mL of methanol, add 50 mg of palladium carbon, fill the flask with hydrogen, stir the reaction mixture at room temperature for 12 h, filter the solution under reduced pressure to remove palladium carbon to obtain NTA, the reaction formula is as follows:

[0061]

[0062] (2) Ni-NTA: take 2mg of NTA and 2.5mL of N...

Embodiment 3

[0075] A paper-based sensor capable of detecting allergic reactions, the preparation method of which is as follows:

[0076] 1.1 Synthesis of Ni-NTA:

[0077] (1) NTA: At 0°C, add 1.6 g of bromoacetic acid into a round bottom flask, and dissolve it with 16 mL of sodium hydroxide. Dissolve 2g of N-carbobenzyloxylysine in 20mL of sodium hydroxide, then add it into the flask, mix well, stir at room temperature for 2h, and then heat to 50°C for 19h. At 0°C, 2.5 mL of concentrated hydrochloric acid was added to the reacted solution to form a precipitate, which was filtered under reduced pressure to form a white powder. Dissolve 2 g of the obtained white powder in 100 mL of methanol, add 200 mg of palladium carbon, fill the flask with hydrogen, stir the reaction mixture at room temperature for 12 h, filter the solution under reduced pressure to remove palladium carbon to obtain NTA, the reaction formula is as follows:

[0078]

[0079] (2) Ni-NTA: take 0.5mg of NTA and 1.25mL o...

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Abstract

The invention relates to a paper based sensor capable of detecting allergic reactions as well as preparation and application of the paper based sensor and belongs to the field of paper based sensors.The allergic reaction refers to abnormal and excessive immune response caused by the reasons that a specific immunoglobulin (IgE) antibody is produced when the body is stimulated by a certain antigenand the IgE can be bound to an IgE specific receptor on the surface of mast cells and basophilic granulocyte. Therefore, the IgE in serum is an important marker for detecting the allergic reaction. Ni-NTA can be specifically bound with a His label, thereby realizing affinity purification of proteins. The paper based material has the advantages of being cheap, portable, high in selectivity and biocompatibility and the like, so the paper based sensor containing the Ni-NTA is prepared and can be firmly bound to an allergen containing the His label, and qualitative detection of the immune globulinis realized. The paper based sensor has important significance for rapidly and sensitively detecting the allergic reactions.

Description

technical field [0001] The invention relates to a paper-based sensor capable of detecting allergic reactions and its preparation and application, belonging to the field of paper-based sensors. Background technique [0002] Paper-based sensors use filter paper as the base to replace silicon, glass, polymers and other materials. By building a hydrophobic isolation zone on the filter paper, the hydrophilic part forms a channel and a detection area to analyze and detect a micro-analysis of the sample. device. It has the advantages of simple preparation, easy portability, low cost, and fast analysis speed, so it has attracted extensive attention in the field of biomedicine in recent years. [0003] The development history of paper-based sensors can be traced back to the 17th century, but it was not until 2007 that paper-based materials developed rapidly. In 2007, the Whitesides research group used photolithography to prepare paper-based sensors for the first time, and combined ...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6854
Inventor 李林魏继福韩希思曹梦妲余昌敏黄维许晨晨张承武于海东刘金华吴琼
Owner NANJING UNIV OF TECH
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