Hemolytic agent and application thereof as well as classifying and counting method for white blood cells

Abstract

The invention relates to the technical field of medical examination and discloses a hemolytic agent and application thereof as well as a classifying and counting method for white blood cells. The hemolytic agent contains one or more than two kinds of cationic surfactants, one or more than two kinds of small molecule alcohols, one or more than two kinds of buffer solutions, one or more than two kinds of soluble inorganic salts and the balance of water. According to the invention, components of the hemolytic agent are simplified; the novel hemolytic agent suitable for classifying and counting basophilic granulocyte and other white blood cells is prepared by adopting the raw materials which are proper and easy to obtain; compared with the current hemolytic agent product with more complex components, the hemolytic agent disclosed by the invention is more convenient and quicker for use; the hemolysis time only needs 5-20s, the classifying and the counting for the basophilic granulocyte and the other white blood cells can be realized by combining the hemolytic agent with an impedance analysis measuring instrument without special requirement on the temperature, and the accuracy is higher.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a hemolyzing agent and its application and a white blood cell classification and counting method. Background technique [0002] White blood cells, also known as white blood cells, are one of the blood cell components. They are colorless round cells, slightly larger than red blood cells, and have nuclei. Can be divided into neutrophils, eosinophils, basophils, lymphocytes and monocytes five. When inflammation or disease occurs in the body, the number of white blood cells often changes, so white blood cell analysis is one of the most commonly used testing items in blood tests in medical laboratories. White blood cell count and classification are not only important for disease diagnosis, differential diagnosis, curative effect observation and prognosis analysis, but also have certain value for the evaluation of health status. [0003] Basophils in white blood cells are abo...

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Problems solved by technology

[0007] Although the technical solution described in the above patent can effectively distinguish various types of leukocytes, the hemolytic agent formula used is relatively complicated and cumbersome, has many components, and is difficult to use Need to follow certain operation steps, less convenient

[0008] US5196346 (Didier Lefevre) discloses a hemolytic agent for measuring leukocytes and basophils, this hemolytic agent uses nonionic surfactant Brij35 and anionic surfactant, but this hemolytic agent requires high temperature for use, The speed of hemolysis is slow, and the source of raw materials is relatively complicated, so it is still not easy to use

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