37 results about "Erythroblast" patented technology

Mithramycin derivatives and their pharmaceutically acceptable salts are disclosed. The mithramycin derivatives can be used in the treatment of Ewing sarcoma or other cancer or neuro-disease associated with an aberrant erythroblast transformation-specific transcription factor.

Described herein is a method of differentiation of pluripotent stem cells into hematopoietic precursor cells, hematopoietic progenitor cells, erythroblast cells, and enucleated erythroblast cells, wherein the method is carried out under suspension agitation, and a GSK-3-inhibitor or a Wnt pathway activator is added during a stage of mesoderm induction. In the preferred embodiment, the pluripotentstem cells are expanded in agitated microcarrier culture and the GSK-3-inhibitior is CHIR99021. Also disclosed herein are cell culture media for use in the methods disclosed herein, as well as kits for performing the same.

There is provided a method for identifying at least one foetal erythroblast the method comprising: (a) detecting the expression of at least one foetal erythroblast specific marker selected from the group consisting of neutral amino acid transporter B (SLC1A5), solute carrier family 3 (activators of dibasic and neutral amino acid transport) member 2 isoform A (SLC3A2), Splice Isoform A of Chloride channel protein 6, Transferrin receptor protein 1, Splice Isoform 3 of Protein GPR107 precursor, Olfactory receptor 11H4, Splice Isoform 1 of Protein C9orf5, Cleft lip and palate transmembrane protein 1, BCG induced integral membrane protein BIGM103, Antibacterial protein FALL-39 precursor, CAAX prenyl protease 1 homolog, Splice Isoform 2 of Synaptophysin-like protein, Vitamin K epoxide reductase complex subunit 1-like protein 1, Splice Isoform 1 of Protein C20orf22 (ABHD12), Hypothetical protein DKFZp564K247 (Hypoxia induced gene 1 protein) (IPI Accession No. IPI00295621), Hypothetical protein DK-FZp586C1924 (IPI Accession No. IPI00031064), ALEX3 protein variant, Hypothetical protein MGC14288 (IPI Accession No. IPI00176708), protein with IPI Accession No. IPI00639803 and protein with IPI Accession No. IPI00646289, wherein detection of the marker indicates the presence of the foetal erythroblast.

The invention discloses a high-throughput rapid classification and analysis method for blood cells suitable for common fish such as crucian carp and carp. Aiming at the problem that nucleated red blood cells that cannot be removed interfere with white blood cell classification and counting in terms of quantity and shape, an automated measurement and analysis strategy combining fluorescent dyes and multi-channel images is used to separate the interference The red blood cell samples are separated from the white blood cells, and the range of different white blood cell groups that undertake important immune functions such as lymphocytes, monocytes, and granulocytes is divided, and automatic analysis is completed. The difficulty of effectively removing nucleated red blood cells has long plagued the automatic analysis of blood images of many types of animals. This invention combines fluorescent dyes with image analysis, and uses the high-throughput and high-sensitivity measurement modules of the multi-dimensional panoramic analyzer to accurately identify red blood cell groups. Eliminating its interference provides important technical support for focused analysis of leukocyte groups and has broad application prospects.

The invention discloses a method for separating and identifying nucleated erythrocytes in blood, which comprises the following steps: (1) preparing reagents; (2) preparing anti-degumming glass slides; Operation method to obtain nucleated red blood cells in the blood. The method for separating and identifying nucleated red blood cells in blood of the present invention can effectively identify nucleated red blood cells, all 40 samples can detect nucleated red blood cells, the detection rate is 100%, and 2×106 to 6×106 nucleated red blood cells were found in each case ml. The present invention is easy to identify nucleated red blood cells after benzidine staining, and can quickly obtain single nucleated red blood cells by skilled micromanipulation, laying a good preliminary foundation for applying single nucleated red blood cells to gene analysis.

The present invention provides a human cell population that can self-renew extensively and yet retain the capacity to differentiate into red blood cells (RBCs). These cells are referred to as extensively self-renewing erythroblasts (ESREs). The cells of the invention serve among other things as a renewable source of transfusable RBCs.