Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting melamine

A technology of melamine and liquid to be tested, which is applied in the direction of material excitation analysis, fluorescence/phosphorescence, etc., and achieves the effect of simple method

Active Publication Date: 2013-06-26
内蒙古兰格格乳业有限公司
View PDF4 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Eosin Y-neutral red has been used in the determination of protein as an energy transfer system, but the use of this energy transfer system to determine melamine by fluorescence spectroscopy has not been reported yet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting melamine
  • Method for detecting melamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0012] (1) Add 0.0, 0.07, 1.0, 2.0, 3.0, 5.0 mL of 0.2g / L melamine solution to six 10mL colorimetric tubes in sequence, and then add 2.0 mL of 4.0×10 -6 mol / L eosin Y (EY) solution, 0.5mL 2.0×10 -4 mol / L neutral red (NR) solution and 0.5mL pH3.5 Britton-Robinson (Britton-Robinson, B-R) buffer solution, using the solution without melamine as the reagent blank, and then using ultrapure water Set the volume of 6 colorimetric tubes to the scale, shake them well, react in a constant temperature water bath at 30°C for 30 min, and use a 1cm quartz cuvette on a fluorescence photometer, with 520nm as the excitation wavelength, respectively at the wavelength of 538nm Determination of fluorescence value F of melamine-containing solution 538nm and the fluorescence value of the reagent blank F 0 , calculate the fluorescence value difference ΔF 538nm = F 538nm – F 0 value, the fluorescence value difference ΔF 538nm It has a linear relationship with the mass concentration ρ of melami...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting melamine. The method comprises the following steps of: sequentially adding 0.05-5.0mL of 0.1-0.5g / L melamine solution, 1.0-2.5ml of 4.0*10<-6>mol / L eosin Y-solution, 0.5-1.0ml of 2.0*10<-4>mol / L neutral red solution and 0.3-1.0ml of Britton-Robinson buffer solution of which the pH value is 3.5 into a 10ml colorimetric tube, using the solution without melamine as the reagent blank, fixing the volume to a specific scale by using super-pure water, reacting for 30min in a water bath of 30 DEG C, subsequently respectively measuring the fluorescence value F538nm of the melamine-containing solution and the fluorescence value F0 of the reagent blank and calculating the difference value that deltaF538nm is equal to F538nm subtracted by the F0 value at 538nm on a fluorophotometer by using a 1cm quartz cuvette in a mode that 520nm is used as the excitation wavelength; preparing 30ml of milk, adding 20.0ml of 1% trichloroacetic acid solution and 8.0ml of 2% lead acetate solution, and carrying out ultrasound treatment for 20min; centrifuging a part of the solution for 10min, taking 1ml of the supernate, and measuring the fluorescence value and calculating the content of the melamine in the milk according to the same way. The method is simple, convenient and rapid.

Description

technical field [0001] The invention relates to a method for determining melamine, in particular to a method for determining melamine by energy transfer fluorescence spectroscopy. Background technique [0002] Melamine is an important triazine nitrogen-containing heterocyclic organic compound, which is often used as a chemical raw material and cannot be used in food processing or food additives. Since the Sanlu milk powder incident occurred in China in 2008, the state has stipulated that the limit value of melamine in liquid milk (including raw milk), milk powder and other formula milk powder is 2.5mg / kg, and products higher than 2.5mg / kg are not allowed to be sold. . Therefore, it is of great significance to study and establish a fast, accurate and low-cost new method for the determination of melamine in milk. At present, the determination methods of melamine are mainly high performance liquid chromatography, gas chromatography-mass spectrometry, liquid chromatography-mas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/64
Inventor 唐宁莉李欣蒙华毅
Owner 内蒙古兰格格乳业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com