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Culture medium capable of simultaneously enriching five kinds of food-borne pathogenic bacteria and preparation method for culture medium

A culture medium and enrichment technology, which is applied in the direction of biochemical equipment and methods, bacteria, microorganism measurement/inspection, etc., can solve the problems of decreased accuracy rate, decreased vitality, and no detection, so as to save costs, simplify operating procedures, The effect of improving detection efficiency

Active Publication Date: 2012-09-12
SOUTHWEST UNIVERSITY FOR NATIONALITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, when using these technologies, two important factors that affect detection must be taken into consideration: first, food is obtained through processing, deep processing, freezing, packaging, storage, etc. will be damaged, the vitality will be reduced
In this case, the accuracy rate drops when using molecular biological methods such as PCR and gene chips. In order to prevent missed detection of food with pathogenic bacteria, the food needs to be enriched before testing.
Second, many modern assays have detection limits in the 10 2 ~10 4 cfu / ml range
[0007] So far, there is no report on the co-enrichment culture technology for the simultaneous enrichment of Salmonella, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Shigella

Method used

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  • Culture medium capable of simultaneously enriching five kinds of food-borne pathogenic bacteria and preparation method for culture medium
  • Culture medium capable of simultaneously enriching five kinds of food-borne pathogenic bacteria and preparation method for culture medium
  • Culture medium capable of simultaneously enriching five kinds of food-borne pathogenic bacteria and preparation method for culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Peptone 10.0g, disodium hydrogen phosphate dodecahydrate 9.0g, potassium dihydrogen phosphate 1.5g, sodium chloride 10.0g, glucose 3.0g, aescin 1.0g, bile salt 0.1g, lithium chloride 1.0g, manna Alcohol 2.0g and sodium pyruvate 2.5g, add distilled water to 990mL, heat to dissolve, cool to room temperature, correct pH value to 7.1-7.3; mix well, sterilize at 121°C for 20min; weigh 0.10mg potassium tellurite and add Add it to 10mL of sterilized distilled water to obtain a potassium tellurite solution; add the prepared potassium tellurite solution under sterile conditions; aliquot and store at 4-6°C for later use.

[0028] Dilute the prepared culture medium for compound enrichment to 10 -4 Salmonella, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Shigella five target bacteria liquid 0.5mL, cultured at 37°C for 24h. The OD value at 540 nm was measured with a UV-Vis spectrophotometer. At the same time, the bacterial solution was inserted into the sele...

Embodiment 2

[0034]Peptone 10.0g, sodium hydrogen phosphate dodecahydrate 9.0g, potassium dihydrogen phosphate 1.5g, sodium chloride 15.0g, glucose 3.0g, aescin 1.0g, bile salt 0.1g, lithium chloride 1.5g, manna Alcohol 6.0g and sodium pyruvate 1.0g, add distilled water to 990mL, heat to dissolve, cool to room temperature, correct the pH value to 7.1-7.3; mix well, sterilize at 121°C for 20min; weigh 0.20mg of potassium tellurite Add it to 10mL of sterilized distilled water to obtain a potassium tellurite solution; add the prepared potassium tellurite solution under sterile conditions; aliquot and store at 4-6°C for later use.

[0035] Dilute the prepared culture medium for compound enrichment to 10 -4 Salmonella, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Shigella five target bacteria liquid 0.5mL, cultured at 37°C for 24h. The OD value at 540 nm was measured with a UV-Vis spectrophotometer. At the same time, the bacterial solution was inserted into the selectiv...

Embodiment 3

[0040] Peptone 10.0g, sodium hydrogen phosphate dodecahydrate 9.0g, potassium dihydrogen phosphate 1.5g, sodium chloride 15.0g, glucose 3.0g, aescin 1.0g, bile salt 0.1g, lithium chloride 1.5g, manna Alcohol 6.0g and sodium pyruvate 5.0g, add distilled water to 990mL, heat to dissolve, cool to room temperature, correct the pH value to 7.1-7.3; mix well, sterilize at 121°C for 20min; weigh 0.05mg of potassium tellurite Add it to 10mL of sterilized distilled water to obtain a potassium tellurite solution; add the prepared potassium tellurite solution under sterile conditions; aliquot and store at 4-6°C for later use.

[0041] Dilute the prepared culture medium for compound enrichment to 10 -4 Salmonella, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Shigella five target bacteria liquid 0.5mL, cultured at 37°C for 24h. The OD value at 540 nm was measured with a UV-Vis spectrophotometer. At the same time, the bacterial solution was inserted into the selecti...

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Abstract

The invention relates to a culture medium for simultaneous composite enrichment of five kinds of food-borne pathogenic bacteria, namely Salmonella, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Shigella, and a preparation method for the culture medium. Food-borne pathogenic bacteria are a significant reason to cause food positioning, so the rapid and accurate detection of the food-borne pathogenic bacteria has an important practical significance for preventing and controlling food safety incidents. The culture medium is characterized by comprising the following components: 10.0 g of peptone, 10.0 g of sodium chloride, 9.0 g of disodium hydrogen phosphate, 1.5 g of monopotassium phosphate, 0.1 g of cholate, 0.1 mg of potassium tellurite, 1.0 g of lithium chloride, 3.0 g of glucose, 2.0 g of mannitol, 2.5 g of sodium pyruvate, 1.0 g of aesculin and 1,000 mL of distilled water, wherein the pH value is 7.1 to 7.5. The culture medium can simultaneously enrich five kinds of target pathogenic bacteria, can be used for separation and identification of target bacteria, can also be used for the molecular detection of multiple pathogenic bacteria on the same detection platform, provides technical support for a method for rapidly detecting five kinds of pathogenic bacteria in food, and meets the requirement of simultaneous detection of five kinds of food-borne pathogenic bacteria.

Description

technical field [0001] The invention belongs to a pre-enrichment culture medium for food-borne pathogenic bacteria, in particular to a culture medium for compound enrichment of Salmonella, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Shigella at the same time and a preparation method thereof. Background technique [0002] Foodborne illness is the most prevalent public health problem worldwide. The World Health Assembly passed a resolution on food safety in 2006, which considered that foodborne diseases have seriously affected the health and well-being of people all over the world, and have caused serious economic losses to individuals, families, communities, businesses and countries[ 1]. Among them, the pathogenic bacteria contaminated in food is one of the main factors causing foodborne diseases, and it is very important to identify foodborne pathogenic bacteria during food poisoning outbreaks[2]. [0003] Salmonella Enteritidis, Escherichia coli O1...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/14C12Q1/10C12Q1/04
CPCY02A50/30
Inventor 唐俊妮陈娟赵燕英顾怀颖李海红王琼
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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