45 results about "ICSI - Intracytoplasmic sperm injection" patented technology

The present invention relates generally to microelectromechanical systems (MEMS) devices for the manipulation of cells or groups of cells, such as oocytes, embryos, and sperm. In particular, the present invention relates to Cell Labeling MEMS devices (2F), Microinjection MEMS devices, IntraCytoplasmic Sperm Injection (“ICSI”) MEMS devices, Zona Coring MEMS devices, Enucleation MEMS devices, Enucleation / Nuclear Transfer MEMS devices, and Cytoplasmic Transfer MEMS devices. The present invention also relates to kits containing the MEMS devices of the present invention.

The invention provides a purpose for preparing supplementary reproduction medicine from the following raw material medicine in percentage by weight: 18 to 30 parts of radix rehmanniae preparata, 9 to 12 parts of dogwood, 8 to 16 parts of Chinese yam, 9 to 12 parts of fructus lycii, 8 to 16 parts of semen cuscutae and 8 to 16 parts of antler gum. The invention also provides a method for promoting ovarian ovum in vitro maturation. The method comprises the following steps that a, an immature ovarian ovum is taken; and b, the immature ovarian ovum is placed in M199 culture liquid, a microdrop method is adopted for culture for 24 hours, and a mature ovarian ovum is obtained, wherein 2 percent of medicine is contained in the culture liquid. The medicine can promote the growth and development of the immature ovarian ovum in vitro and mainly promotes the ovarian ovum core maturation, and in addition, the cytoskeleton abnormality probability of the mature ovarian ovum is not increased. The medicine can also generate the influence on the ovum passing capability of sperms, and the reliable basis is provided for clinically preventing and treating the male sperm dysfunction, improving the IVF (in vitro fertilization) success ratio and reducing the use rate of ICSI (intracytoplasmic sperm injection).

A method for producing a monkey-derived embryonic stem cell comprising the steps of carrying out fertilization by insemination by in vitro fertilization or intracytoplasmic sperm injection using a monkey ovum and monkey sperms, thereby giving a fertilized ovum, allowing the fertilized ovum to differentiate into a blastocyst by in vitro culture, and establishing an ES cell line using the blastocyst; the monkey ES cell obtained by the method, a method for screening a reagent for specific differentiation into cell or tissue by using the ES cell; and a differentiated cell or differentiated tissue each differentiated from the ES cell. According to the present invention, applications of the embryonic stem cells to embryological studies clinical applications, experimental models, and the like on primates, studies of diseases, are expected.

A container or dish used for the micro manipulation, micro injection, biopsy and fertilization of oocyte and embryo culture. The dish allows the user to more readily perform procedures used to fertilize oocyte, such as intracytoplasmic sperm injection (ICSI), biopsy embryos and perform additional procedures in used in assisted reproductive techniques (ART), human reproduction and in vitro fertilization (IVF) techniques. The invention will allow ease in use, the reduction in the number of micro tools used in the procedure, as opposed to conventional dishes and procedures, and will allow the user to more readily locate the oocytes and embryos to be handled and worked on. The invention will add repetitiveness, consistency and may result in better results and outcome of the procedures. The invention will also give the user a more ergonomically correct dish for these types of procedures and related protocols.

The present invention relates to systems and methods for automated, computer-controlled, tracking and manipulation of motile specimen. The systems and methods of the present invention are suited for tracking and manipulating sperm, particularly for Intracytoplasmic Sperm Injection (ICSI) procedures. The present invention also relates also to devices for holding motile cells, such as sperm, and non-motile cells, such as oocytes. The device of the present invention comprises a sealed chamber having an air outlet, one well for the motile cells, and another well for non-motile cells.

The invention provides a purpose for preparing supplementary reproduction medicine from the following raw material medicine in percentage by weight: 18 to 30 parts of radix rehmanniae preparata, 9 to 12 parts of dogwood, 8 to 16 parts of Chinese yam, 9 to 12 parts of fructus lycii, 8 to 16 parts of semen cuscutae and 8 to 16 parts of antler gum. The invention also provides a method for promoting ovarian ovum in vitro maturation. The method comprises the following steps that a, an immature ovarian ovum is taken; and b, the immature ovarian ovum is placed in M199 culture liquid, a microdrop method is adopted for culture for 24 hours, and a mature ovarian ovum is obtained, wherein 2 percent of medicine is contained in the culture liquid. The medicine can promote the growth and development of the immature ovarian ovum in vitro and mainly promotes the ovarian ovum core maturation, and in addition, the cytoskeleton abnormality probability of the mature ovarian ovum is not increased. The medicine can also generate the influence on the ovum passing capability of sperms, and the reliable basis is provided for clinically preventing and treating the male sperm dysfunction, improving the IVF (in vitro fertilization) success ratio and reducing the use rate of ICSI (intracytoplasmic sperm injection).

An integrated automated system comprising a microfluidic cassette, and methods of use thereof, for intracytoplasmic sperm injection assisted fertilization. The microfluidic cassette and the integrated automated system provides a complete set-up of human gametes for assisted in vitro fertilization, including proper cell stage recognition, gamete propulsion via microfluidic currents, microinjection of a spermatozoon into an oocyte, and subsequent embryo culture and monitoring, thus allowing widespread distribution of in vitro insemination by favoring affordability.