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Microelectromechanical devices useful for manipulating cells or embryos, kits thereof, methods of making same, and methods of use thereof

a micro-electromechanical and embryonic technology, applied in the field of micro-electromechanical systems (mems) devices for the manipulation of cells or embryos, can solve the problems of insufficient optimization, poor viability of cultured oocytes and embryos, and the enormity of the demand placed on technical staff by these procedures, so as to facilitate the identification of an individual oocyte, facilitate the identification of a single oocyte, and facilitate the effect of cell manipulation speed and

Inactive Publication Date: 2009-01-29
MATHEWS SHEPHERD MCKAY & BRUNEAU P A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The MEMS devices facilitate rapid and precise manipulation of cells, increasing success rates in assisted reproduction and animal husbandry procedures by enabling automated handling, labeling, and tracking of oocytes and embryos, thereby reducing the workload on technical staff and improving procedural efficiency.

Problems solved by technology

At present the outcome of these procedures, being technically demanding and relatively novel and as such, not optimized, is very poor.
The rigors of the physical manipulation of these cells during the generation of transgenic animals, intracytoplasmic sperm injection, assisted hatching, enucleation, nuclear transfers and cytoplasmic transfer as well as the sheer enormity of the demand that these procedures place on technical staff represents two of the main reasons for failure.

Method used

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  • Microelectromechanical devices useful for manipulating cells or embryos, kits thereof, methods of making same, and methods of use thereof
  • Microelectromechanical devices useful for manipulating cells or embryos, kits thereof, methods of making same, and methods of use thereof
  • Microelectromechanical devices useful for manipulating cells or embryos, kits thereof, methods of making same, and methods of use thereof

Examples

Experimental program
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Effect test

examples

[0698]The following protocols and experimental methods and materials are employed in the Examples that follow.

Superovulation

[0699]Mice (i.e., RB Swiss, (CBA*C57BL6 / J)fl) are given 5 i.u. / ml Pregnant Mares Serum Gonadotrophin (PMSG) interperitoneally (i.p.). At 46 to 48 hours post injection a second injection is i.p. administered providing 5 i.u. / ml Human Chorionic Gonadotrophin (hCG) in Phosphate Buffered Saline (PBS). If mating is desired, females are placed with males immediately.

Blastocyst Flush

[0700]The uterus is removed from 3.5 day pregnant mice and placed into sterile PBS. Using a sterile fine forceps the mesometrium is trimmed and the ovaries, oviducts, the utero-tubal junction, and the cervical bifurcation are dissected from both of the uterine horns. The uterine horn is flushed using a syringe of DMEM (Dulbecco's Modification of Eagle's Medium (Mod.) 1× (DMEM) with L-Glutamine, 4.5 g / L Glucose and Sodium Pyruvate; Fisher Scientific cat. # MT10013CM) with HEPES with a blunt...

example

The In Vitro Culture Device Operation of In Vitro Culture Device

[0745]After having been placed in an environmental controlling instrument of the present invention, the single layer or multi-layer in vitro culture device is loaded with fluids (i.e., culture media), any bubbles being purged by gentle pressure being applied to the system through the loading / removal compartments or through the input and output enclosed channels. Oocytes or embryos, having been attached to labelable zona anchor MEMS devices that are attractive to the movement tracks of the in vitro culture device, are added to the loading compartment (i.e., by mouth pipette, by robotic means, by other cell handling means). The environmental controlling instrument CPU is provided with the desired culture conditions, time, and amendment parameters. The environmental controlling instrument CPU actuates the travel of the cells on the movement tracks through out the single or multi-layer in vitro culture device, provides cult...

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Abstract

The present invention relates generally to microelectromechanical systems (MEMS) devices for the manipulation of cells or groups of cells, such as oocytes, embryos, and sperm. In particular, the present invention relates to Cell Labeling MEMS devices (2F), Microinjection MEMS devices, IntraCytoplasmic Sperm Injection (“ICSI”) MEMS devices, Zona Coring MEMS devices, Enucleation MEMS devices, Enucleation / Nuclear Transfer MEMS devices, and Cytoplasmic Transfer MEMS devices. The present invention also relates to kits containing the MEMS devices of the present invention.

Description

[0001]The present application is a continuation-in-part application of U.S. Provisional Application No. 60 / 130,802 filed Apr. 23, 1999; U.S. Provisional Application No. 60 / 147,802 filed Aug. 9, 1999, and U.S. Provisional Application No. 60 / 149,269 filed Aug. 17, 1999. The disclosures of the above-identified applications are incorporated herein by reference in their entirety.FIELD OF THE INVENTION[0002]The present invention relates generally to microelectromechanical systems (MEMS) devices for the manipulation of cells or groups of cells, such as oocytes, embryos, and sperm. In particular, the present invention relates to Cell Labeling MEMS devices, Labelable Zona Anchor MEMS devices, Microinjection MEMS devices, IntraCytoplasmic Sperm Injection (“ICSI”) MEMS devices, Zona Coring MEMS devices, Enucleation MEMS devices, Enucleation / Nuclear Transfer MEMS devices, and cytoplasmic transfer MEMS devices. The present invention also relates to kits containing the MEMS devices of the present...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/00C23F1/00C12M1/00H01L21/02B23P15/00B44C1/22C12MC12M1/22C12M1/34C12M3/00C12N5/00C12N5/08C12N11/08C12N11/14D04B9/00D04B9/20
CPCB01L3/5027C12M35/04C12M21/06
Inventor PALACIOS-BOYCE, MONICA
Owner MATHEWS SHEPHERD MCKAY & BRUNEAU P A
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