Method for producing transgenic buffalo embryos by applying intracytoplasmic sperm injection (ICSI) mediation
A transgenic, buffalo technology, applied in the field of genetically engineered transgenic animals, can solve the problems of low milk production and reproduction rate, restricting the development of buffalo milk industry, etc.
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Embodiment 1
[0022] From 2008 to 2009, among a batch of 102 injected eggs, 63 split (61.8%), 38 blastomere EGFP-expressed embryos (60.3%), and 17 blastocysts were obtained, of which 11 blastocysts EGFP was expressed, and the transgenic positive rate of blastocysts was as high as 64.7%. One of the transgenic embryos was cultured in vitro for a long time, and the expression of EGFP could still be seen at 27 days, which proved that the application of this method can effectively produce transgenic buffalo embryos .
Embodiment 2
[0024] Between 2008 and 2009, we injected 95 oocytes, and after activation, 86 of them split (90.5%), 36 of which expressed EGFP (41.9%), and 16 blastocysts were obtained after 7 days of in vitro culture. 10 of them expressed EGFP (62.5%). This example proves that the method of the present invention for producing transgenic buffalo embryos through ICST-mediated method is feasible.
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