35results about How to "Avoid depolymerization" patented technology

The invention is concerned with a kind of rubber disintegrator. There are two roller canisters set in type of revolving on axis seat and they move inside of the roller. The axis of roller canister is fixed on frame of machine through the bearing seat, and the motor and reducer is droved by the revolving on axis seat and they move inside of the roller. The axis of roller canister is fixed on frame of mach axis of the two roller canisters. The said roller canister is made up of some slice type of drills on the axis with lengthways congruence and the drills of two roller canisters are interleaving. The roller canisters move to different directions and send the top of rubber block between the two roller canisters into the drills for cutting. The structure to exit of stuff is outer screen; with exit of stuff and the main part wrapped by the outer screen is the main body of the two roller canisters. The outer screen is a column and the side of it is the screen, the two bottom of it has circumferential rim and the bottom of each rim on the revolving axis seat is driven by transmission framework.

The invention discloses a preparation method of a hydrophobic charge induction chromatography medium with large capacity, and belongs to the protein chromatography separation technology in the field of biochemical engineering. The chromatography medium refers to the end of a space arm that is coupled with imidazol compounds as a chromatography matrix. The preparation method is that: the medium is activated after dimethyl sulfoxide, epichlorohydrin and sodium hydroxide solution are sequentially put into agarose gel medium; the activated medium is coupled with the imidazol compounds matrix in the alkaline dimethyl sulfoxide solution; finally, the epoxy left on the medium surface gets the needed chromatography medium after being reduced. The adsorption capacity of the chromatography medium to the protein achieves a moist medium of 80mg/ml and keeps relatively stable within a broader ionic strength scope from 0.1 mol/L to 1.0 mol/L; at the same time, the protein elutropic model which is finished by depending on the regulation of PH between 4.0 and 6.0 is more moderate, thereby having the wide application prospect in the separation and purification of biomacromolecule such as the protein, etc.

The invention discloses immobilized maleic acid cis-trans isomerase and a preparation method and application thereof, and belongs to the technical fields of enzymology and enzyme engineering and the field of biochemical industries. According to the immobilized maleic acid cis-trans isomerase and the preparation method and application thereof, R5 peptide fragments are connected to the N ends of the maleic acid cis-trans isomerase, R5-MaiA is crosslinked through glutaraldehyde, embedding is conducted on the crosslinked R5-MaiA by utilizing silicon reaction liquid, and immobilization of the maleic acid cis-trans isomerase is completed. The immobilized maleic acid cis-trans isomerase (R5-MI-CLEA-Si) with high stability is obtained through the method which is low in cost and easy to operate, the optimum reaction temperature of the immobilized isomerase is 55 DEG C, and the optimum reaction pH is 7.7; on the condition that the temperature is 55 DEG C, the half-life period of the immobilized isomerase is 4 h, the half-life period of the free isomerase is 0.5 h, the stability of the immobilized isomerase is eight times of that of the free isomerase, and after a catalytic reaction is repeated for eight times, the enzyme activity of the immobilized isomerase can be still retained at 75%.

The invention provides a preparation method of high-performance polysulfone resin. The preparation method includes adding 45-55 parts of 4,4-dichlorodiphenylsulfone, 35-40 parts of salt-forming agent, 80-100 parts of low boiling point organic solvent and 0.01-0.04 parts of 3-fluoro-4-chlorobenzophenone into the reactor, and then 20-60 parts of 4,4'- -dihydroxydiphenylsulfone or bisphenol, injecting nitrogen gas and heating until the temperature inside the reactor rises to 200-210 DEG C, maintaining reaction at constant temperature for 1 to 2 hours, and after the temperature rises to 240-260 DEG C for 4 to 7 hours, adding 0.09-0.16 parts of 3-fluoro-4-chloro benzophenone, reacting 10-20 minutes, then stopping reactor and treating the reactant to obtain high-performance polysulfone resin. The polysulfone resin prepared by the method has high molecular weight, good transparency and heat resistance.

Methods for use of a multi-arm polyethylene glycol (PEG) modifier in modification of asparaginase. The described multi-arm PEG modifier enhances the subunit interaction of a multimeric protein to maintain the multimeric protein in a polymerized form, thereby improving the stability of the multimeric protein, maintaining the bioactivity of the multimeric protein, and reducing the probability of exposure of the antigen binding site after depolymerization of the subunits, so as to reduce the immunogenicity.

The invention discloses a preparation method of 3-carboxymethyl-1,2,4-cyclopentanetricarboxylic acid-1,4:2,3-dianhydride and belongs to the field of preparation methods of fine chemicals. The main technical scheme is that dicyclopentadiene is taken as a starting material and subjected to oxidation reaction with ozone and hydrogen peroxide sequentially, intermediate product 2,3,5-tricarboxylic acidcyclopentane acetic acid is obtained, finally, acetic anhydride is taken as a dehydrating agent, and dehydration ring closing is performed for synthesizing the target product 3-carboxymethyl-1,2,4-cyclopentanetricarboxylic acid-1,4:2,3-dianhydride. The method in the scheme has the advantages of being mild in reaction condition, safe to operate, high in product purity and yield, pollution-free andthe like, and is suitable for large-scale industrial production.

The invention relates to the technical field of slow-release nitrogen fertilizer production. The invention further discloses a process method for preparing the agricultural slow-release nitrogen fertilizer by crushing and regenerating the waste amino resin. The preparation method comprises the following steps: preparing choline chloride and urea into eutectic ionic liquid, activating and degradingthe crushed powdery waste amino resin and processing the amino resin into amino resin slow-release nitrogen fertilizer liquid, and granulating and drying the nitrogen fertilizer liquid to finally obtain the amino resin slow-release nitrogen fertilizer. The initial nitrogen release rate of the slow-release nitrogen fertilizer is 1.5-2 times of the nitrogen release rate of common urea formaldehyde,the effective slow-release total amount is about 2-2.5 times of that of common urea formaldehyde, the effective release time is prolonged by 3-4 weeks, and the slow-release nitrogen fertilizer has agood slow-release effect.

The invention relates to the use of CXCL4L1 as a biomarker of pancreatic cancer in a patient. More particularly, the invention relates to a method for detecting a pancreatic cancer and/or pancreatic metastasis in a patient, said method comprising determining the expression level of the CXCL4L1 gene in a biological sample obtained from said patient.

The invention discloses a preparation method of a compound protein surimi product, and belongs to the technical field of meat food processing. The preparation method comprises the steps of fish cream preparation, surimi preparation, core material preparation, core-wrapped surimi preparation and shell layer preparation. The preparation method has the advantages that a trypsin agent is added into surimi, so that denaturation of myofibrillar protein under the freezing condition can be inhibited, and in the curing process, cross-linking with whey protein and myofibrillar protein can be realized, so that the elasticity of the surimi is improved; the core material is coated with the surimi, so that the edible pleasure of the surimi product can be greatly improved, and the cheese, the yolk liquid, the maltodextrin and the cured soybean paste are blended, so that the dissolution of fat-soluble substances can be effectively avoided; and the slurry is molded and cured into a film on the surface of the surimi, so that a good water retaining effect on the surimi is achieved, and the retention rate of fat-soluble substances in the core material can be well realized.