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Injectable hydrogel with antibacterial, antioxidant and hemostatic effects as well as preparation method and application of injectable hydrogel

An anti-oxidation and water injection technology, used in pharmaceutical formulations, pharmaceutical sciences, prostheses, etc., can solve problems such as poor mechanical properties and rapid degradation, and achieve poor mechanical strength, good biocompatibility, and no cytotoxicity. Effect

Pending Publication Date: 2022-03-08
SURGSCI SHENZHEN MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its own shortcomings such as too fast degradation and poor mechanical properties, it cannot be used simply as a hemostatic material.

Method used

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  • Injectable hydrogel with antibacterial, antioxidant and hemostatic effects as well as preparation method and application of injectable hydrogel
  • Injectable hydrogel with antibacterial, antioxidant and hemostatic effects as well as preparation method and application of injectable hydrogel
  • Injectable hydrogel with antibacterial, antioxidant and hemostatic effects as well as preparation method and application of injectable hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] The present embodiment provides a kind of quaternized chitosan, and preparation method is as follows:

[0079] Dissolve 0.5g of chitosan (Mw=200000-1500000, degree of deacetylation=90%) into deionized water, and then add 20mL of glacial acetic acid aqueous solution (concentration: 0.05mol / L) dropwise while stirring. React for 180 minutes, then add 0.8g 2,3-epoxypropyltrimethylammonium chloride, react at 55°C for 18 hours, centrifuge the reaction solution at a speed of 4000r / min for 10 minutes to obtain a supernatant, add 5 times the volume ethanol, the precipitation is complete, and finally suction-filtered and dried to obtain a quaternized chitosan polymer. Its reaction route is as follows figure 1 shown.

Embodiment 2

[0081] This embodiment provides a kind of oxidized hyaluronic acid, the preparation method is as follows:

[0082] Dissolve 2g of hyaluronic acid (Mw=100000-2000000) in 100mL of deionized water. After it is completely dissolved, add 25mL of 0.04mol / L sodium periodate aqueous solution dropwise while stirring, and then react for 4 hours at 25°C in the dark. After the reaction, 5 mL of ethylene glycol was added to terminate the reaction; the reaction solution was put into a dialysis bag, put into ultrapure water for dialysis for 4 days, and the ultrapure water was changed every other time, and the oxidized hyaluronic acid was obtained after freeze-drying. things. Its reaction route is as follows figure 2 shown.

Embodiment 3

[0084] This embodiment provides an injectable hydrogel, the preparation method of which is as follows:

[0085] The quaternized chitosan polymer that embodiment 1 is prepared is mixed with the quaternized chitosan solution that mass concentration is 30mg / mL with PBS damping fluid; The oxidized hyaluronic acid that embodiment 2 is made is buffered with PBS The solution was prepared into a solution with a mass concentration of 50 mg / mL; the volume ratio of the quaternized chitosan solution and the oxidized hyaluronic acid solution was 100:35. Mix the oxidized hyaluronic acid solution and the quaternized chitosan solution evenly.

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Abstract

The invention relates to injectable hydrogel with antibacterial, antioxidant and hemostatic effects and a preparation method and application thereof, the preparation method comprises the following steps: mixing an oxidized hyaluronic acid solution and a tannic acid solution, then mixing with a quaternized chitosan solution, and carrying out Schiff base reaction polymerization to obtain the injectable hydrogel. The hydrogel overcomes the defects that hyaluronic acid chitosan hydrogel in the prior art is poor in mechanical strength, rapid in degradation and the like; gel is formed in situ through Schiff base reaction, a cross-linking agent is not needed, and the method is green and safe; the device can be used for injection at a wound part, and has strong adaptability to wounds with irregular shapes; and the gel is degradable, so that a secondary operation is avoided. The polyphenol free radical scavenging activity of the tannic acid can effectively inhibit wound secondary oxidative stress injury caused by ROS (reactive oxygen species) and is beneficial to wound antibiosis and inflammation diminishing, meanwhile, the tannic acid can coagulate damaged collagen and provide an effective in-vivo hemostatic effect, and more importantly, after the tannic acid is introduced, the mechanical strength of the gel can be further enhanced.

Description

technical field [0001] The invention belongs to the technical field of biological tissue engineering materials, and relates to an injectable hydrogel and its preparation method and application, in particular to an injectable hydrogel with antibacterial, anti-oxidation and hemostatic effects, and its preparation method and application. Background technique [0002] Medical hemostatic material is a material that can quickly stop bleeding after a large amount of blood occurs in the patient's wound. Traditional hemostatic materials such as inorganic minerals (zeolite, montmorillonite, etc.), surface wound bandages, gauze, sponges, etc. have low hemostatic ability and cannot maintain a moist environment for a long time, resulting in slow wound healing. In modern medical technology research, various biomaterials have been used for rapid wound healing, including electrospun nanofibers, porous foams, biocompatible films, and functional hydrogels. [0003] Among these materials, hyd...

Claims

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Application Information

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IPC IPC(8): A61L26/00A61L27/58A61L27/54A61L27/52A61L27/26C08J3/24C08J3/075C08L5/08C08B37/08
CPCC08J3/246C08J3/075A61L26/008A61L26/0066A61L26/0052A61L27/52A61L27/54A61L27/26A61L27/58A61L26/009C08J2305/08C08J2405/08A61L2400/06A61L2300/236A61L2300/404A61L2300/418C08L5/08
Inventor 陈晨冯耿超
Owner SURGSCI SHENZHEN MEDICAL TECH CO LTD
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