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Automatic sperm floating apparatus and use method thereof

A sperm upstream device and automatic technology, applied in the field of upstream pipes, can solve the problems such as no method for sucking out motile sperm, no avoidance of culture medium, no solution, etc., and achieves the effects of convenient management and operation, avoiding contamination of culture medium, and convenient operation.

Inactive Publication Date: 2013-12-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But it also does not avoid the risk of contamination of the culture medium with semen, nor does it propose a method for aspirating motile sperm, and does not address the above-mentioned deficiencies

Method used

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  • Automatic sperm floating apparatus and use method thereof
  • Automatic sperm floating apparatus and use method thereof
  • Automatic sperm floating apparatus and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] see Figure 1 ~ Figure 3 , this embodiment includes a liquid inlet pipe 1, a liquid outlet pipe 2 and a pipe body 3, the upper end of the pipe body 3 is tapered, the top of the tapered pipe body 3 is connected to the liquid outlet pipe 2, and the liquid outlet pipe 2 is connected to the pipe body 3 Internal communication; the liquid inlet pipe 1 is fixed to the side wall of the pipe body 3, the bottom end of the liquid inlet pipe 1 communicates with the bottom of the pipe body 3, and the upper end of the liquid inlet pipe 1 is provided with a cap 5. The culture fluid and semen 7 enter the bottom of the tube body 3 successively from the liquid inlet pipe 1. Because the density of the semen 7 is relatively high, the seminal fluid 7 that enters later is located at the lower part of the culture fluid, and the semen 7 will not pollute the culture fluid during the liquid feeding process; After cultivating upstream and entering the culture solution, squeeze the tube body 3 by ...

Embodiment 2

[0042] see Figure 4 , In this embodiment, the liquid inlet pipe 1 is fixed to the outer wall of the pipe body 3, and a through hole 9 is opened between the bottom end of the liquid inlet pipe 1 and the side wall of the pipe body 3 to communicate with each other. The liquid inlet pipe 1 is located outside the pipe body 3, and there is no interface between the liquid inlet pipe 1 and the top of the pipe body 3, so as to ensure that the inside of the pipe body 3 is sealed. All the other structures and functions are the same as in Embodiment 1.

[0043] In this embodiment, the upper end of the liquid inlet pipe 1 does not need to pass through the tube body 3, and there is no hole at the upper end of the tube body 3. When the tube body 3 is squeezed, the culture solution 6 will not overflow from the upper end of the tube body 3.

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Abstract

The invention relates to an automatic sperm floating apparatus. The apparatus comprises a liquid inlet tube, a liquid outlet tube and a tube body, the upper end of the tube body has a conical shape, the top of the conical tube body is connected with the liquid outlet tube, and the liquid outlet tube is communicated with the internal of the tube body; the liquid inlet tube is fixed to a sidewall of the tube body, the bottom of the liquid inlet tube is communicated with the bottom of the tube body, and the upper end of the liquid inlet tube is provided with a tube cover; and the materials of the liquid inlet tube, the liquid outlet tube and the tube body are a deformable nontoxic transparent plastic. The apparatus has the advantages of reasonable structure design, no need of extra suction tubes for the addition of a culture liquid and a semen and the suction of a culture liquid containing motile sperms, material consumption saving, convenient and controllable operation, furthest reduction of the culture liquid pollution possibility, and guarantee of the cleanliness and purity of the motile sperms obtained through a floating method.

Description

technical field [0001] The invention relates to an automatic sperm upstream device and its application method, which is an upstream tube used for upstream sperm in an assisted reproductive technology laboratory. Background technique [0002] With the emergence and promotion of assisted reproductive technology, the treatment effect of infertility has been greatly improved. In assisted reproductive technologies including intrauterine insemination (IUI) and in vitro fertilization-embryo transfer (IVF-ET) and their derivatives, the isolation of motile sperm and the removal of immotile sperm and other components of seminal plasma The semen processing process is an important part. At present, the semen processing process mainly includes gradient centrifugation and upstream method. Due to the cumbersome operation of gradient centrifugation and greater damage to sperm, the upstream method is more popular. In the ordinary upstream semen processing process, it is necessary to add ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N5/076
Inventor 卢永超杨静黄荷凤
Owner ZHEJIANG UNIV
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