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Animal semen preservative with biological activity and preparation method thereof

A technology of biological activity and preservative, which is applied in the field of animal semen preservative and its preparation to achieve the effects of improving sperm survival rate, maintaining animal sperm motility and prolonging storage time.

Inactive Publication Date: 2014-03-26
LINYI SIKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current preservatives lack antioxidant components that prevent semen from being oxidized by free radicals and reducing its activity.

Method used

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  • Animal semen preservative with biological activity and preparation method thereof
  • Animal semen preservative with biological activity and preparation method thereof
  • Animal semen preservative with biological activity and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0024] A preparation method of a biologically active animal semen preservative, comprising the following steps:

[0025] S1. Prepare two powdery media, at least one of the two powdery media contains at least one oligomeric proanthocyanidin;

[0026] S2. mixing the two powdery media together;

[0027] S3. Dilute the mixture of the two powdery media into a solution with pure water;

[0028] S4. Add the pig semen sample to the solution, preferably immediately after collection;

[0029] S5. Balance the osmotic pressure of the semen sample with the solution, and then divide it into a certain volume for storage. OPCs can slow down the degradation of semen and have no negative impact on sperm motility. In addition, the pH value of the preservation solution is lower than 7.5, and the osmotic pressure is about Below 350m0sm.

[0030] Further, the above powder medium may contain at least two, three or four oligomeric proanthocyanidins.

[0031] The preparation process of the present...

Embodiment 2

[0041] Mix Batesville thawing solution (BTS) with grape seed extract to form a biologically active animal semen preservative, and make the concentration of grape seed extract in the preservative 0mg / L (control), 60mg / L, 130mg / L L or 250mg / L. Sperm motility was detected at 0, 7, 15, 19, 28 and 37 hours of storage, and the storage temperature was 37°C. The average number of sperm motility was obtained by combining the sperm motility at different times, and the results are summarized in Table 2. Under the conditions of high metabolic rate and high free radical production, sperm motility was higher after treatment with OPCs.

[0042] Table 2

[0043]

example 3

[0045] Use Westendorf medium (11% lactose, 25% egg yolk) as cryopreservation protection agent, and choose the optimum concentration of grape seed extract 16mg / L. Aliquot the collected fresh semen into the protectant and centrifuge. The semen was then cryopreserved with or without the addition of OPCs. After cryopreservation in liquid nitrogen, thaw at 50°C for 15s, and then assess sperm motility. The results obtained are listed in Table 3, and the results show that the survival rate and the integrity of the cell membrane of spermatozoa after cryopreservation were significantly improved after being treated with OPCs.

[0046] table 3

[0047] deal with

[0048] The biologically active animal semen preservative of the present invention is also suitable for preserving egg cells and embryos of various animals, including pigs, cattle, sheep and the like.

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Abstract

The invention aims to provide an animal semen preservative with biological activity and a preparation method of the animal semen preservative. The animal semen preservative with the biological activity contains oligoprocyanidine so that the animal semen preservative has an anti-oxidation effect, can prolong the preservation time of semen and keeps the activity of animal sperms. The animal semen preservative is prepared by mixing 1000mL of a common medium and 16mg of oligoprocyanidine; the common medium is prepared by mixing 6 parts of glucose, 0.37 part of EDTA (Ethylene Diamine Tetraacetic Acid), 0.375 part of sodium citrate dihydrate, 0-0.12 part of sodium bicarbonate, 0.1 part of neomycin and 100 parts of distilled water; the neomycin is independently added before the semen is diluted. The formula contains the oligoprocyanidine so that the animal semen preservative has the anti-oxidation effect and can prolong the preservation time of the semen; the degradation of a semen sample can be relieved by the oligoprocyanidine and the oligoprocyanidine has no negative effects on the activity of the sperms, so that the activity of the animal sperms is kept; particularly, the survival rate of the sperms preserved at a low temperature is obviously improved.

Description

technical field [0001] The invention relates to a biologically active animal semen preservative and a preparation method thereof. Background technique [0002] Artificial insemination (AI) technology, together with in vitro fertilization and embryo transfer technology, has greatly improved the reproductive efficiency of animals. The above technique has many advantages over natural mating. As far as livestock breeding is concerned, the above techniques can spread the desired genetic characteristics faster and wider. The semen of one male animal can inseminate multiple female animals, and the number of male animals required for artificial insemination is only a quarter of the number of male animals required for natural mating, thus saving feeding costs. Moreover, artificial insemination can better control the entire population, improve reproductive efficiency, and facilitate large-scale production. [0003] The key to artificial insemination and in vitro fertilization is to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 刘洋
Owner LINYI SIKE BIOTECH
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