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A kind of SNP marker related to pig birth weight traits and its application

A marker and body weight technology, applied in the field of pig breeding, can solve the problems of long breeding cycle and slow genetic progress, and achieve the effects of reducing breeding costs, increasing birth weight, and improving survival rate.

Active Publication Date: 2022-05-31
SHENZHEN HUADA GENE INST +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional breeding methods mostly use traditional phenotypic breeding techniques, that is, to infer parental productivity based on offspring phenotype information, long breeding cycle, and slow genetic progress

Method used

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  • A kind of SNP marker related to pig birth weight traits and its application
  • A kind of SNP marker related to pig birth weight traits and its application
  • A kind of SNP marker related to pig birth weight traits and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0038] 1. Acquisition of SNP loci related to porcine birth weight

[0039] In this case, a total of 674 sows from 453 large whites and 221 landraces were selected, and the weight of their offspring piglets at 2 hours after birth was weighed by standard weight scales as the phenotypic value of the pig's primary weight trait. The research on the SNP loci related to the birth weight of pigs is as follows:

[0040] 1. Genomic DNA Extraction

[0041] Tissue samples the size of soybeans were collected and stored in 75% ethanol for future use. DNA extraction was carried out using the blood / tissue DNA magnetic bead extraction kit from Changchun Zhiang Biotechnology Co., Ltd., the product number is GO-BTCD-400, and the extraction was carried out according to the standard procedure of the instructions. Extracted DNA was applied to INVITROGEN Qubit TM dsDNAHS Assay Kit was used for quantification and stored at -20°C.

[0042] 2. Porcine whole genome sequencing and typing

[0043] R...

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Abstract

The application discloses a SNP marker related to pig birth weight traits and its application. The SNP marker related to the birth weight trait of pigs in this application is located at the 112031589 nucleotide site on chromosome 4 of the pig international genome version 11.1 reference sequence, and has a G or A polymorphism. The SNP markers of the present application related to pig birth weight traits can be used for birth weight prediction of piglets and molecular marker-assisted selection breeding of pigs. Breeding using the SNP markers of the present application can not only shorten the breeding time and improve the accuracy of breeding; it is not easily affected by other external environment and other factors; it can more effectively increase the birth weight of piglets, thereby improving the survival rate, weaning weight and fattening The effect is of great significance to the breeding and production of pigs. Using the SNP marker of the present application for assisted breeding can carry out early selection and timely elimination, reduce breeding costs, quickly obtain homozygous parents, and improve breeding efficiency and quality.

Description

technical field [0001] The present application relates to the technical field of pig breeding, in particular to a SNP marker and application related to the pig's birth weight trait. Background technique [0002] Pork is rich in protein, vitamins and minerals, and is the main non-staple food in the daily life of Chinese residents. Although my country's population growth rate is slowing down, the population is still in a state of continuous growth, and the demand for pork will also maintain a growing state. Therefore, the breeding industry needs to improve the supply capacity of high-quality pork. [0003] The weight of piglets at birth, that is, the birth weight of pigs, is an important quantitative trait to measure pigs, which affects the growth and development, production performance and carcass grade of pigs throughout their lives. Pig birth weight was positively correlated with the slaughter weight of pork, and negatively correlated with the slaughter age. According to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124Y02P60/87
Inventor 王然杨漫漫魏强
Owner SHENZHEN HUADA GENE INST
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