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Kit and method for family division and paternity identification of domestic pigs

A paternity test and kit technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as division and few paternity test reports.

Active Publication Date: 2021-09-10
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few reports on family division and paternity identification of domestic pigs using SSR technology, especially Anqing Liubai family division and paternity identification for Anhui's unique germplasm resources.

Method used

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  • Kit and method for family division and paternity identification of domestic pigs
  • Kit and method for family division and paternity identification of domestic pigs
  • Kit and method for family division and paternity identification of domestic pigs

Examples

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Embodiment 1

[0025] 1 Material method:

[0026] 1.1 Sample collection

[0027] The present invention collected 98 purebred Anqing Liubai pig ear tissue samples in total, provided by Anhui Huating Lake Green Food Development Co., Ltd. (Chengling Village, Jinxi Town, Taihu County, Anqing City, Anhui Province). The ear samples were stored in a 1.5mL centrifuge tube filled with 500μL of 75% alcohol, and stored at -20°C after being brought back to the laboratory.

[0028] 1.2 DNA extraction

[0029] The tissue DNA of Anqing Liubai pig was extracted using Adler Biotechnology Company (Beijing) Tissue Genomic DNA Rapid Extraction Kit, and the extracted DNA samples were checked for concentration and purity using an ultraviolet spectrophotometer, and qualified DNA samples were stored at -80 ℃ environment for standby.

[0030] 1.3 Microsatellite site selection and primer design

[0031] The information of the 14 sites selected in the present invention is shown in Table 1, and the primer sequences...

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Abstract

The invention belongs to the technical field of livestock molecular biology, and provides a kit and method for family division and paternity identification of domestic pigs. The kit and method comprises the following steps: specifically selecting 14 SSR loci of domestic pigs, especially Anqing Liubai pigs, synthesizing corresponding primers for the loci, conducting capillary electrophoresis detection on ear tissue DNA of 98 Anqing Liubai pigs, counting and calculating the number of effective alleles, heterozygosity, polymorphic information content, genetic distance and exclusion rate of each locus, obtaining family division of the domestic pigs, especially the Anqing Liubai pigs, and performing paternity identification. The cumulative elimination probability of 14 microsatellite loci reaches 99%. The 14 microsatellite loci selected by the research have polymorphism in the Anqing Liubai pig population and can be used as effective genetic markers to be applied to production practices such as family division, paternity identification and the like of domestic pigs, especially the Anqing Liubai pig population.

Description

technical field [0001] The invention belongs to the technical field of livestock molecular biology, and relates to a pig family division and paternity identification kit and method. Background technique [0002] The protection of pig breed resources relies on local breed conservation farms. In the production process, there are multiple introductions, unclear records of pedigree and production information, etc., which lead to unclear kinship of pigs in stock and increased risk of inbreeding, which directly leads to high-quality pigs. The number of breeding pig families has decreased, and the purity and genetic diversity of breeds have decreased, seriously affecting the preservation of high-quality breeding pig germplasm resources and industrial development. Therefore, accurate kinship is of great significance for formulating pig breeding plans, avoiding inbreeding, saving seed selection, and speeding up the breeding process. [0003] Simple Sequence Repeats (Simple Sequence ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125C12Q2563/107C12Q2600/124
Inventor 丁月云殷宗俊张晓东吴旭东郑先瑞曹邦基陈琼夏静凌紫荆侯银辉孔诚诚
Owner ANHUI AGRICULTURAL UNIVERSITY
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