53 results about "Genotype Assay" patented technology

Genetic polymorphisms responsible or associated with altered expression of cytochrome P450 CYP3A5 enzyme are described. Single nucleotide polymorphisms are provided. Methods for identifying subjects having a low or high drug metabolizing phenotype associated with CYP3A5 expression are provided. Assays, kits and methods for determining and assaying the CYP3A5 genotype and phenotype of individual patients are disclosed. Oligonucleotide probes and primers for use in the assays, kits and methods are described. Assays and methods for determining and evaluating an individual's metabolism of drugs and therapeutic agents, the potential for drug interactions, and thereby toxicity and effectiveness of certain drugs and treatment modalities, are provided.

The invention provides a classification method, which can be used for quickly and conveniently classifying a genotype of a fad2 gene of cabbage type rape with oleic acid content which is more than 30 percent, and particularly discloses a method for classifying the genotype of the fad2 gene of the cabbage type rape by judging whether basic groups of nucleotides from a forty-first position to a fiftieth position of the fad2 gene of the cabbage type rape are AAAAGTCTGA or not and whether basic groups of nucleotides from a two hundred and thirty-first position to a two hundred and forty-fifth position are TCCCTCACCCTCTCT or not.

Methods for separating pericarp tissue from surrounding tissues in grain are provided. Included are methods for isolation of high-purity pericarp DNA from a grain plant that reflects the genotype of the maternal parent of the grain plant, such that the isolated DNA may be used in a PCR-based genotyping assay.

The invention provides a maize single panicle weight main effect QTL, as well as an acquisition method and application thereof. The QTL comprises qEW-1, qEW-2, qEW-3, qEW-4 and qEW-5. The method comprises the following steps: configuring cross combination by taking a high-yield early-maturity maize inbred line SG5 as a female parent and a maize inbred line SG7 with relatively poor yield trait as a male parent, and constructing an F2 genetic group of the cross combination; performing GBS sequencing-basing typing on the F2 genetic group, and performing genotyping by combining differential SNP validated based on SG5 and SG7 parents; investigating the yield trait of single F2 panicle, performing QTL analysis on the F2 single panicle yield trait by utilizing a winQTLcart2.5 software composite interval mapping method, and analyzing the chromosome region of the main effect QTL and the genetic effect. According to the method, a theoretic base is provided to excavating and controlling the maize single panicle weight main effect QTL and linked marker, a molecular marker closely linked with the target QTL is acquired, and foundation is laid to maize single panicle yield trait QTL candidate gene prediction, cloning and molecular mark assisted breeding.

The invention provides a double PCR method applied to identification of herring kinship, comprising the following steps: (1) extracting genomic DNA of a herring sample; (2) performing double PCR using 5 sets of microsatellite primers; (3) PCR amplification The products were separated by electrophoresis on polyacrylamide gel; (4) The genotypes of PCR amplification products were counted to analyze the genetic relationship between individuals. The five sets of microsatellite primers are all sites with high polymorphism and clear bands, which can realize the genetic diversity detection and individual identification of herring, and can also identify the distance of genetic relationship between individuals, so as to avoid the herring individual Between inbreeding, the time and cost are reduced by half compared with the traditional PCR reaction. It has been verified by experiments that this method can be used to identify the genetic relationship of 30 herrings, and the genetic relationship between each sample can be accurately identified, and the genetic distance between the samples can be calculated according to the identification results.

Methods and compositions are described for use in the rapid and simultaneous screening of one or more samples for one or more polymorphisms in the TIGR gene. The methods and compositions of the present invention can be used to rapidly determine if polymorphisms in a gene encoding the TIGR protein are present in the genome of a subject. Identifying which polymorphisms are present in an individual can permit the diagnosis or prediction of the risk of glaucoma in the subject.

The present invention provides, for the first time, the finding that the manganese superoxide dismutase Val16Ala polymorphism is significantly associated with prognosis for cancer patients treated with chemotherapeutic drug therapy. The alanine allele is a novel biomarker that predicts poor response and poor outcome to chemotherapeutic drug cancer therapy. Conversely, the valine allele predicts a good response and a good outcome to chemotherapeutic drug cancer therapy. Therefore, a genotype assay can be used to determine which alleles a subject is carrying, and subsequently this information can be used to determine if chemotherapeutic drug therapy is appropriate, and to customize therapy according to the patient's MnSOD genotype.